1. Lactation and gestation controls on calcium isotopic compositions in a mammalian model
- Author
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Doryan Grivault, Samuel Le Goff, Vincent Balter, Jean-Alexis Hernandez, Auguste Hassler, Emmanuelle Albalat, Stéphane Ferchaud, Jeremy E. Martin, Théo Tacail, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Oslo (UiO), Center for Earth Evolution and Dynamics, University of Oslo, School of Earth Sciences [Bristol], University of Bristol [Bristol], Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Calcium Isotopes ,Swine ,media_common.quotation_subject ,[SDV]Life Sciences [q-bio] ,Biophysics ,chemistry.chemical_element ,[SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny ,Fractionation ,Calcium ,010502 geochemistry & geophysics ,01 natural sciences ,Biochemistry ,Models, Biological ,Bone and Bones ,Biomaterials ,03 medical and health sciences ,Animal science ,Pregnancy ,Lactation ,[SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology ,medicine ,Animals ,0105 earth and related environmental sciences ,media_common ,Isotope ,Metals and Alloys ,Animal Feed ,Body Fluids ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Animals, Newborn ,Chemistry (miscellaneous) ,Gestation ,Colostrum ,Composition (visual arts) ,Female ,Reproduction - Abstract
Lactation and gestation are among the physiological events that trigger the most intense changes in body calcium (Ca) fluxes. Along with the composition of the animal 2021 diet, these events are suspected to impact the Ca isotopic composition of Ca body reservoirs but their dynamics are poorly understood. In this study, we monitored a group of domestic sows across a full reproduction cycle. We collected tissues and fluids (blood, urine, milk, colostrum, umbilical blood, adult and piglet bones) at different steps of gestation and lactation, and analyzed their Ca isotopic compositions (i.e. δ44/42Ca) by means of multi-collector inductively coupled plasma mass spectrometry. Among other results, we report the first observations of Ca isotopic fractionation between maternal and umbilical blood (Δ44/42Caumbilical blood-sow blood = −0.18 ± 0.11‰, n = 3). Our data also highlight that gestation and lactation periods are characterized by small diet-bone Ca isotopic offsets (Δ44/42Cabone-diet = −0.28 ± 0.11‰, n = 3), with 44Ca-enriched blood compositions during nursing (Δ44/42Canursing blood-gestation blood = $+ 0.42{\rm{\,\,}}_{ - 0.12}^{ + 0.11}$‰, n = 3). Under the light of an up-to-date mammalian box model, we explored different scenarios of gestation and lactation Ca fluxes experienced by a sow-like animal. These simulations suggest that gestation changes on body δ44/42Ca values may result from the intensification of Ca absorption by the animal, whereas the production of 44Ca-depleted milk is the main driver for the 44Ca enrichment in blood during lactation. In addition, our results also support that bone mineralization could be associated with a more restricted Ca isotopic fractionation than previously envisioned. Together, these results refine the framework of Ca isotope applications, notably regarding the monitoring of human bone balance and the study of species and ecosystems from the present and the past.
- Published
- 2020
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