1. The uncoupling protein 2 modulates the cytokine balance in innate immunity
- Author
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Corinne Hurtaud, Yalin Emre, Sophie Rousset, Anne-Marie Cassard-Doulcier, Daniel Ricquier, Olivier Join-Lambert, Biologie des Transporteurs Mitochondriaux et Métabolisme (BIOTRAM), Centre National de la Recherche Scientifique (CNRS), Pathogénie des infections systémiques (UMR_S 570), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biologie des Transporteurs Mitochondriaux et Métabolisme ( BIOTRAM ), Centre National de la Recherche Scientifique ( CNRS ), Pathogénie des infections systémiques ( UMR_S 570 ), and Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS )
- Subjects
Chemokine ,Phagocyte ,MESH: Spleen ,MESH : Cytokines ,medicine.medical_treatment ,MESH : Reactive Oxygen Species ,MESH : Immunity, Natural ,MESH: Phagocytes ,MESH: Listeria monocytogenes ,Biochemistry ,MESH: Mice, Knockout ,Ion Channels ,Mice ,0302 clinical medicine ,Immunology and Allergy ,Macrophage ,Uncoupling Protein 2 ,MESH: Animals ,MESH : Macrophages ,Mice, Knockout ,Phagocytes ,0303 health sciences ,MESH: Cytokines ,biology ,MESH: Reactive Oxygen Species ,MESH: Mitochondrial Proteins ,Hematology ,3. Good health ,Interleukin 10 ,medicine.anatomical_structure ,Cytokine ,MESH : Phagocytes ,Cytokines ,MESH : Ion Channels ,MESH : Spleen ,Immunology ,MESH : Mice, Inbred C57BL ,In Vitro Techniques ,Mitochondrial Proteins ,03 medical and health sciences ,Immune system ,Immunity ,MESH: Mice, Inbred C57BL ,MESH : Mice ,medicine ,Animals ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Molecular Biology ,MESH: Mice ,MESH : Mitochondrial Proteins ,030304 developmental biology ,MESH: Immunity, Natural ,Innate immune system ,Macrophages ,MESH: Macrophages ,Listeria monocytogenes ,Immunity, Innate ,Mice, Inbred C57BL ,MESH: Ion Channels ,biology.protein ,MESH : Mice, Knockout ,MESH : Animals ,MESH : Listeria monocytogenes ,Reactive Oxygen Species ,Spleen ,030217 neurology & neurosurgery - Abstract
The uncoupling protein 2 (UCP2) is located in the inner mitochondrial membrane and downregulates the production of reactive oxygen species (ROS). Recent data suggested a role for UCP2 in the immune response. We analyzed further this hypothesis during acute Listeria monocytogenes infection in mice. Death of infected Ucp2(-/-) mice was delayed in comparison with Ucp2(+/+), suggesting a role of UCP2 in the early step of the immune response. In vitro, the higher resistance of Ucp2(-/-) mice was not associated with a better control of bacterial growth by macrophages. In vivo, a significant increase of recruited phagocytes was observed in the spleen of Ucp2(-/-) mice. This was associated with a higher level of ROS in the spleen. Upregulation of pro-inflammatory cytokines IFNgamma, IL6, and IL1beta and of the chemokine MCP1 was observed in Ucp2(-/-) mice 4 days after infection, preceded by a decrease of the anti-inflammatory cytokine IL10 production. Present data highlight that, in an acute model of infection, UCP2 modulates innate immunity, via the modulation of ROS production, cytokine and chemokine production and consequently phagocyte recruitment.
- Published
- 2006
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