1. Spatial turnover of fungi and partner choice shape mycorrhizal networks in epiphytic orchids
- Author
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Rémi Petrolli, Lucie Zinger, Benoît Perez‐Lamarque, Géromine Collobert, Chantal Griveau, Thierry Pailler, Marc‐André Selosse, Florent Martos, Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Naturalis Biodiversity Center [Leiden], Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Gdańsk (UG), Department of Plant Taxonomy and Nature Conservation, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), This work was supported by the Agence Nationale de la Recherche (ANR-19-CE02-0002). Sampling in the protected area was authorized by the Reunion National Park (DIR-I-2020-013). Logistic support was provided by the field station of Marelongue, funded by the P.O.E., Reunion National Park and OSU Reunion. We also thank Claudine Ah-Peng, Audrey Valery and Jocelyne Glaudert for logistic support during the fieldwork, Soraya Chaouch from the ‘plateau technique de Cytométrie en flux et QPCR, Plateforme analytique du MNHN (UMR7245)’ and Céline Bonillo for laboratory assistance., ANR-19-CE02-0002,EPIFUN,L'épiphytisme en tant qu'écosystème fongique colonisé par les plantes vasculaires(2019), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Petrolli, Rémi, and L'épiphytisme en tant qu'écosystème fongique colonisé par les plantes vasculaires - - EPIFUN2019 - ANR-19-CE02-0002 - AAPG2019 - VALID
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Ecology ,[SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis ,Plant Science ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,Ecology, Evolution, Behavior and Systematics ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,[SDV.BV.BOT] Life Sciences [q-bio]/Vegetal Biology/Botanics ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis - Abstract
The peer review history for this article is available at https://publons.com/publon/10.1111/1365-2745.13986.Raw data have been deposited in the Sequence Read Archive (SRA) under the BioProject accession no. PRJNA692353.; International audience; In soils, plants and fungi can form complex mycorrhizal networks allowing nutrient transfers between plant individuals and species. It is less clear, however, whether such networks exist on the bark of trees where epiphytic plant communities thrive in rainforests. Previous work showed that tropical epiphytic orchids especially, harbour symbiotic fungi in their roots, but the structure and determinants of the resulting networks remain unknown at the tree scale.We tested the hypothesis that epiphytic orchids rooted in the same area on the bark share mycorrhizal fungi, regardless of their species (i.e. spatial determinant). For this purpose, we selected the trunk of six trees of two common species in a rainforest and sampled orchid roots, protocorms and surrounding bark. We identified mycorrhizal fungi including Tulasnellaceae using high-throughput sequencing of the ITS2 marker, and reconstructed orchid-fungus bipartite networks for each tree to analyse their structure and the spatial turnover of this symbiosis.We found that epiphytic orchid communities form antinested and highly modular networks with mycorrhizal fungi spread on the bark. As expected, modules of interactions are explained by their spatial structure, with nearby roots sharing fungi, but also by the orchid species involved. These results reveal the presence of shared mycelial networks in epiphytic habitats, whose roles in the resilience and facilitation of epiphytic plant communities need to be assessed.Synthesis. Tropical tree barks are densely colonized by certain mycorrhizal fungi that can form symbioses in nearby adult and young orchids simultaneously. These mycorrhizal networks may allow water and nutrient transfers to alleviate the stressful conditions of the epiphytic habitats.
- Published
- 2022
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