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In order to explore the extraction technology and antioxidant function analysis of polysaccharides from walnut peel, in this study, ethanol/K2HPO4 was used to extract polysaccharides from walnut peel. The extraction rate of polysaccharide was used as the index. On this basis, response surface Box-Behnken coupled genetic algorithm was used to optimize the two-water phase extraction process of walnut peel polysaccharide. The structure was characterized by Fourier Transform Infrared Spectroscopy and its antioxidant activity was studied in vitro. The results showed that the optimal extraction process of polysaccharides from walnut peel by dual-phase technology was as follows: in the dual-phase system, the lower phase K2HPO4 was 16% mass fraction, the dual-phase extraction time was 34 min, and the dual-phase extraction temperature was 28 ℃, so the WPPs yield of 111 mg/g could be achieved. The polysaccharides from walnut peel had characteristic absorption peaks of polysaccharide compounds. The polysaccharides of walnut peel prepared by aqueous two-phase method has significant scavenging effect on ABTS free radicals, the two-phase aqueous system can extract polysaccharides from walnut peel security, inexpensive and effectively, and retain the biological activity of polysaccharides from walnut peel. [ABSTRACT FROM AUTHOR]

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