Search

Ｏｂｊｅｃｔｉｖｅ Ｔｏ ｓｔｕｄｙ ｔｈｅ ｃｈａｒａｃｔｅｒｉｓｔｉｃｓ ｏｆ ＴＣＭ ｓｙｎｄｒｏｍｅｓ ｉｎ ｅｌｄｅｒｌｙ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｏｓｔｅｏｐｏｒｏｔｉｃ ｆｒａｃｔｕｒｅｓ. Ｍｅｔｈｏｄｓ Ａ ｔｏｔａｌ ｏｆ ８０ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｏｓｔｅｏｐｏｒｏｔｉｃ ｆｒａｃｔｕｒｅｓꎬ ｗｈｏ ｗｅｒｅ ｉｎ ｔｈｅ ｏｒｔｈｏｐｅｄｉｃ ｗａｒｄ ｏｆ Ｌｏｎｇｈｕａ Ｈｏｓｐｉｔａｌ ｆｒｏｍ Ｏｃｔｏｂｅｒ ２０１５ ｔｏ Ｊａｎｕａｒｙ ２０１６ꎬ ｗｅｒｅ ｓｅｌｅｃｔｅｄ ａｎｄ ｉｎｖｅｓｔｉｇａｔｅｄ ｗｉｔｈ ｑｕｅｓｔｉｏｎｎａｉｒｅｓ. Ｂｏｎｅ ｍｉｎｅｒａｌ ｄｅｎｓｉｔｙ ｏｆ ｔｈｅ ｈｅｅｌ ｗａｓ ｍｅａｓｕｒｅｄ ｗｉｔｈ ｃａｌｃａｎｅａｌ ｂｏｎｅ ｄｅｎｓｉｔｏｍｅｔｅｒ. Ｔｈｅ ｄａｔａ ｒｅｌａｔｅｄ ｗｉｔｈ ＴＣＭ ｓｙｎｄｒｏｍｅｓ ｗｅｒｅ ａｎａｌｙｚｅｄ ａｎｄ ｅｘｃａｖａｔｅｄ. Ｒｅｓｕｌｔｓ Ｔｈｅｒｅ ｗｅｒｅ ３７ ｃａｓｅｓ ｏｆ ｓｐｌｅｅｎ￣ｋｉｄｎｅｙ ｙａｎｇ ｄｅｆｉｃｉｅｎｃｙ ａｃｃｏｕｎｔｉｎｇ ｆｏｒ ４６􀆰 ２５％ ｏｆ ｔｈｅ ｔｏｔａｌꎬ １３ ｃａｓｅｓ ｏｆ ｌｉｖｅｒ￣ｋｉｄｎｅｙ ｙｉｎ ｄｅｆｉｃｉｅｎｃｙ ａｃｃｏｕｎｔｉｎｇ ｆｏｒ １６􀆰 ２５％ꎬ ａｎｄ １４ ｃａｓｅｓ ｏｆ ｑｉ ｓｔａｇｎａｔｉｏｎ￣ｂｌｏｏｄ ｓｔａｓｉｓ ａｃｃｏｕｎｔｉｎｇ ｆｏｒ １７􀆰 ５０％. Ｔｈｅ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｓｐｌｅｅｎ ａｎｄ ｋｉｄｎｅｙ ｙａｎｇ ｄｅｆｉｃｉｅｎｃｙ ｗｅｒｅ ｐｒｏｎｅ ｔｏ ｆｒａｃｔｕｒｅｓ ａｔ ｌｅａｓｔ ｔｗｉｃｅ ｏｒ ｍｏｒｅ ｔｉｍｅｓꎬ ｅｓｐｅｃｉａｌｌｙ ｉｎ ｆｅｍａｌｅｓ ｗｉｔｈ ｌｏｎｇｅｒ ｐｅｒｉｏｄ ａｆｔｅｒ ｍｅｎｏｐａｕｓｅꎬ ｗｈｏ ｍａｉｎｌｙ ｓｕｆｆｅｒｅｄ ｆｒｏｍ ｈｉｐ ｏｒ ｓｐｉｎｅ ｆｒａｃｔｕｒｅｓ. Ｃｏｎｃｌｕｓｉｏｎ Ｅｌｄｅｒｌｙ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｏｓｔｅｏｐｏｒｏｓｉｓ ｏｆｔｅｎ ｓｕｆｆｅｒ ｆｒｏｍ ｆｒａｃｔｕｒｅｓ ｄｕｅ ｔｏ ｆａｌｌｉｎｇꎬ ｃａｕｓｉｎｇ ｂｙ ｄｅｃｒｅａｓｅ ｏｆ ｍｕｓｃｌｅ ｃｏｏｒｄｉｎａｔｉｏｎ. Ｓｐｌｅｅｎ ｇｏｖｅｒｎｓ ｍｕｓｃｌｅ ａｎｄ ｋｉｄｎｅｙ ｇｏｖｅｒｎｓ ｂｏｎｅ. Ｍｕｓｃｌｅ￣ｓｋｅｌｅｔａｌ ｓｙｓｔｅｍ ｂｅｃｏｍｅｓ ｗｅａｋ ｗｈｉｌｅ ｓｐｌｅｅｎ ａｎｄ ｋｉｄｎｅｙ ａｒｅ ｄｅｆｉｃｉｅｎｔ. Ｔｈｕｓ ｔｈｅ ｔｈｅｏｒｉｅｓ ｏｆ ｓｐｌｅｅｎ￣ｋｉｄｎｅｙ ｃｏｒｒｅｌａｔｉｏｎ ａｎｄ ｃｏｎｇｅｎｉｔａｌ ａｎｄ ａｃｑｕｉｒｅｄ ｏｒｉｇｉｎ ｐｌａｙ ｋｅｙ ｒｏｌｅｓ ｉｎ ｐｒｅｖｅｎｔｉｏｎ ａｎｄ ｔｒｅａｔｍｅｎｔ ｏｆ ｏｓｔｅｏｐｏｒｏｔｉｃ ｆｒａｃｔｕｒｅｓ. [ABSTRACT FROM AUTHOR]

Objective To study the curative mechanism of the invigorating spleen and nourishing kidney formula on promoting osteoporotic fracture healing，and to discuss the traditional Chinese medicine theories of the correlation between spleen and kidney and the origin of the innateness and acquirement in the application of the osteoporotic fracture prevention． Methods The osteoporotic fracture model was established in ovariectomized mice． The mice were randomly divided into normal saline-treated group and invigorating spleen and nourishing kidney formula-treated group at three time points ( 7，14，and 28 days) ． The mice were administered with corresponding drugs by gastric lavage on the second day after the fracture surgery． They were executed at the three time points after the treatment． Samples were collected for image observation and histopathological staining． Ｒesults The result of X-ray and micro-CT showed that the formation of callus and fracture healing were promoted in the invigorating spleen and nourishing kidney formula group at 7，14，and 28 days． Micro-CT analysis showed that BV /TV，Conn． D，mean /density of TV， and mean /density of BV in the invigorating spleen and nourishing kidney formula group were significantly higher than those in normal saline group ( P ＜ 0. 05) at 7 and 14 days． The finite element analysis suggested that the stiffness of fracture position and the elasticity modulus in invigorating spleen and nourishing kidney formula group were significantly higher than those in normal saline group at 7 days ( P ＜ 0. 05) ． The results of HE and ABH/OG staining showed that the invigorating spleen and nourishing kidney formula promoted intramembranous and endochondral ossification to accelerate fracture healing． The results of immunohistochemistry showed that the invigorating spleen and nourishing kidney formula increased the expression of β-catenin and Ｒunx2 to promote osteoporotic fracture healing． Conclusion The invigorating spleen and nourishing kidney formula effectively promotes osteoporotic fracture healing and the mechanism may be related to the increase of β-catenin and Ｒunx2 expression． [ABSTRACT FROM AUTHOR]