1. Analytical Quality by Design-based development and validation of ultra pressure liquid chromatography/MS/MS method for glycopeptide antibiotics determination in human plasma
- Author
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Jelena Maksić, Guro Forsdahl, Ana Stajić, Biljana Jančić-Stojanović, Đoko Maksić, and Mirjana Medenica
- Subjects
0301 basic medicine ,medicine.drug_class ,030106 microbiology ,Clinical Biochemistry ,Antibiotics ,UPLC/MS/MS ,01 natural sciences ,High-performance liquid chromatography ,Quality by Design ,Mass Spectrometry ,Analytical Chemistry ,03 medical and health sciences ,Vancomycin ,medicine ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Chromatography, High Pressure Liquid ,plasma ,Chromatography ,Chemistry ,Teicoplanin ,010401 analytical chemistry ,General Medicine ,AQbD ,Glycopeptide ,0104 chemical sciences ,Anti-Bacterial Agents ,Medical Laboratory Technology ,Human plasma ,Uplc ms ms ,glycopeptide antibiotics ,medicine.drug - Abstract
Aim: An ultra pressure liquid chromatography (UPLC)/MS/MS method for vancomycin and teicoplanin determination in human plasma was developed in accordance with analytical quality by design (AQbD) concept and fully validated. Materials & methods: Chromatographic separation was performed on ACQUITY UPLC C18 charge surface hybrid (CSH) column (2.1 mm × 50 mm, 1.7 μm particle size) in gradient mode and the mobile phase consisted of 0.1% formic acid in water and pure acetonitrile. The experimental design methodology was used for the definition of optimal chromatographic and protein precipitation conditions. Results: The linearity ranges were 0.05–10 μg ml-1 for vancomycin and 0.5–200 μg ml-1 for total teicoplanin. The relative standard deviations for precision estimation were below 15% and the accuracy was within 85–115% for all quality control levels. Conclusion: The method was utilized for glycopeptide antibiotics bioanalysis.
- Published
- 2018