Search

Your search keyword '"Guillaume Née"' showing total 7 results
Start Over Author "Guillaume Née" Topic 01 natural sciences
7 results on '"Guillaume Née"'

1. A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone Regulation

Author

Pascale Satour, Guillaume Née, Emmanuelle Issakidis-Bourguet, Gilles Châtel-Innocenti, Juliette Leymarie, Françoise Montrichard, Christophe Bailly, Patrice Meimoun, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biologie du Développement [IBPS] (LBD), Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Gestionnaire, HAL Sorbonne Université 5, Laboratoire Interdisciplinaire des Energies de Demain (LIED (UMR_8236)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Sorbonne Université (SU), Université Paris-Est Créteil Val-de-Marne - Faculté des sciences et technologie (UPEC FST), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-10LABX-0040-SPS, and ANR-17-EUR-0007.

Subjects
0106 biological sciences, F. Satour, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Physiology, 01 natural sciences, Thioredoxins, P. Bailly, Plant Growth Regulators, Gene Expression Regulation, Plant, P. Leymarie, Plastids, Biology (General), Spectroscopy, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Hormone inhibitor, 0303 health sciences, P, food and beverages, General Medicine, J. Montrichard, Phenotype, Computer Science Applications, organ, Chemistry, G. Châtel-Innocenti, redox, Seeds, C. Issakidis-Bourguet, Cell signaling, G. Meimoun, QH301-705.5, Satour, Germination, Leymarie, Biology, Catalysis, Article, Inorganic Chemistry, C, 03 medical and health sciences, G, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Montrichard, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Physical and Theoretical Chemistry, Plastid, Molecular Biology, Gene, QD1-999, 030304 developmental biology, E. A New Role for redox non-photosynthetic organ, non-photosynthetic organ, Arabidopsis Proteins, Née, Meimoun, Organic Chemistry, Bailly, J, biology.organism_classification, redoxnon-photosynthetic, Issakidis-Bourguet, F, E. A New Role for redox, Function (biology), Châtel-Innocenti, 010606 plant biology & botany
Abstract

International audience; In Arabidopsis seeds, ROS have been shown to be enabling actors of cellular signaling pathways promoting germination, but their accumulation under stress conditions or during aging leads to a decrease in the ability to germinate. Previous biochemical work revealed that a specific class of plastid thioredoxins (Trxs), the y-type Trxs, can fulfill antioxidant functions. Among the ten plastidial Trx isoforms identified in Arabidopsis, Trx y1 mRNA is the most abundant in dry seeds. We hypothesized that Trx y1 and Trx y2 would play an important role in seed physiology as antioxidants. Using reverse genetics, we found important changes in the corresponding Arabidopsis mutant seeds. They display remarkable traits such as increased longevity and higher and faster germination in conditions of reduced water availability or oxidative stress. These phenotypes suggest that Trxs y do not play an antioxidant role in seeds, as further evidenced by no changes in global ROS contents and protein redox status found in the corresponding mutant seeds. Instead, we provide evidence that marker genes of ABA and GAs pathways are perturbed in mutant seeds, together with their sensitivity to specific hormone inhibitors. Altogether, our results suggest that Trxs y function in Arabidopsis seeds is not linked to their previously identified antioxidant roles and reveal a new role for plastid Trxs linked to hormone regulation.

Published
2021
Full Text
View/download PDF

2. Functional Divergence of the Arabidopsis Florigen-Interacting bZIP Transcription Factors FD and FDP

Author

Seonghoe Jang, Guillaume Née, Hyonhwa Ohr, Coral Vincent, Maida Romera-Branchat, Pedro Madrigal, Fernando Andrés, Chloé Pocard, Edouard Severing, Rafael Martinez-Gallegos, George Coupland, Madrigal, Pedro [0000-0003-1959-8199], Apollo - University of Cambridge Repository, Max Planck Institute for Plant Breeding Research (MPIPZ), University of Münster, World Vegetable Center Korea Office, Développement Adaptatif du Riz [AGAP] (DAR), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Plant Genetics (IPG), Polish Academy of Sciences (PAN), University of Cambridge [UK] (CAM), ERC Advanced Grant HyLife, German Research Foundation (DFG) : EXC 2048/1, 390686111, Max Planck Society, Foundation CELLEX, European Project: 237909,EC:FP7:PEOPLE,FP7-PEOPLE-ITN-2008,SYSFLO(2009), Westfälische Wilhelms-Universität Münster = University of Münster (WWU), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
0301 basic medicine, 0106 biological sciences, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, 01 natural sciences, chemistry.chemical_compound, 0302 clinical medicine, Transcription (biology), Gene Expression Regulation, Plant, Abscisic acid, lcsh:QH301-705.5, Phylogeny, Gene Editing, 0303 health sciences, biology, food and beverages, Plants, Genetically Modified, Cell biology, ChIP-seq, Basic-Leucine Zipper Transcription Factors, ABA, Florigen, FD, Genotype, MADS Domain Proteins, Flowers, FDP, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, FT, florigen, Gene, Transcription factor, 030304 developmental biology, Homeodomain Proteins, flowering, Arabidopsis Proteins, CCAAT-Enhancer-Binding Protein-beta, fungi, 15. Life on land, biology.organism_classification, 030104 developmental biology, chemistry, lcsh:Biology (General), Mutagenesis, bZIPs, CRISPR-Cas Systems, RNA-seq, 030217 neurology & neurosurgery, Functional divergence, Abscisic Acid, Transcription Factors, 010606 plant biology & botany
Abstract

Summary Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genus Arabidopsis contain two of these bZIPs, FD and FDP, which we show have largely complementary expression patterns in shoot apices before and during flowering. CRISPR-Cas9-induced null mutants for FDP flower slightly earlier than wild-type, whereas fd mutants are late flowering. Identical G-box sequences are enriched at FD and FDP binding sites, but only FD binds to genes involved in flowering and only fd alters their transcription. However, both proteins bind to genes involved in responses to the phytohormone abscisic acid (ABA), which controls developmental and stress responses. Many of these genes are differentially expressed in both fd and fdp mutant seedlings, which also show reduced ABA sensitivity. Thus, florigen-interacting bZIPs have distinct functions in flowering dependent on their expression patterns and, at earlier stages in development, play common roles in phytohormone signaling., Graphical Abstract, Highlights • FDP is expressed at the shoot apex in a largely discrete pattern to FD • Flowering genes are bound only by FD, whereas other targets are bound by FD and FDP • FD and FDP bind to and regulate genes involved in ABA responses in seedlings • FT overexpressed in the phloem promotes flowering independently of FD and FDP, Florigen activating complex facilitates the response to day length in higher plants and contains a specific class of bZIP transcription factor. Romera-Branchat et al. analyze the two factors of this class in Arabidopsis and find they play distinct functions in flowering control and participate in ABA signaling in early development.

Published
2020
Full Text
View/download PDF

3. DELAY OF GERMINATION1 requires PP2C phosphatases of the ABA signalling pathway to control seed dormancy

Author

Katharina Kramer, Emma Miatton, Guillaume Née, Yong Xiang, Bingjian Yuan, Kazumi Nakabayashi, Wim J. J. Soppe, and Iris Finkemeier

Subjects
0106 biological sciences, 0301 basic medicine, Science, Phosphatase, Regulator, Arabidopsis, General Physics and Astronomy, Germination, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Botany, Phosphoprotein Phosphatases, Abscisic acid, Multidisciplinary, biology, Arabidopsis Proteins, fungi, Seed dormancy, food and beverages, General Chemistry, biology.organism_classification, Plant Dormancy, 030104 developmental biology, chemistry, Seeds, Dormancy, Plant hormone, 010606 plant biology & botany, Abscisic Acid, Signal Transduction
Abstract

The time of seed germination is a major decision point in the life of plants determining future growth and development. This timing is controlled by seed dormancy, which prevents germination under favourable conditions. The plant hormone abscisic acid (ABA) and the protein DELAY OF GERMINATION 1 (DOG1) are essential regulators of dormancy. The function of ABA in dormancy is rather well understood, but the role of DOG1 is still unknown. Here, we describe four phosphatases that interact with DOG1 in seeds. Two of them belong to clade A of type 2C protein phosphatases: ABA-HYPERSENSITIVE GERMINATION 1 (AHG1) and AHG3. These phosphatases have redundant but essential roles in the release of seed dormancy epistatic to DOG1. We propose that the ABA and DOG1 dormancy pathways converge at clade A of type 2C protein phosphatases., The DOG1 protein is a major regulator of seed dormancy in Arabidopsis. Here, Née et al. provide evidence that DOG1 can interact with the type 2C protein phosphatases AHG1 and AHG3 and that this represents the convergence point of the DOG1-regulated dormancy pathway and signalling by the plant hormone abscisic acid.

Published
2017

4. Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination

Author

Thomas Nietzel, Stephan Wagner, Gernot Poschet, Michael Büttner, Abdelilah Benamar, Anna Moseler, Ian M. Møller, Falko Hochgräfe, Rüdiger Hell, Iris Finkemeier, Stefanie J Müller-Schüssele, Markus Schwarzländer, Cristina Ruberti, Andreas J. Meyer, Guillaume Née, Markus Wirtz, Jörg Mostertz, David Macherel, Christopher Horst Lillig, Philippe Fuchs, Rheinische Friedrich-Wilhelms-Universität Bonn, General Electric Medical Systems [Buc] (GE Healthcare), General Electric Medical Systems, équipe RV&RA, Centre de Robotique (CAOR), MINES ParisTech - École nationale supérieure des mines de Paris-PSL Research University (PSL)-MINES ParisTech - École nationale supérieure des mines de Paris-PSL Research University (PSL), Department of Mathematical Sciences [Matieland, Stellenbosch Uni.] (DMS), Stellenbosch University, Institute of Crop Science and Resource Conservation (INRES), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Molekulkar Pflanzenphysiologie, Universität Erlangen-Nürnberg, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Centre for Organismal Studies [Heidelberg] (COS), Heidelberg University, Plant Proteomics Group, Max Planck Institute for Plant Breeding Research (MPIPZ), Institut für Nutzpflanzenwissenschaften und Ressourcenschutz (INRES), Institut für Biologie und Biotechnologie der Pflanzen, University of Münster, Institute of Crop Science and Resource Conservation [Bonn] (INRES), Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), German Research Foundation (DFG) : SCHW1719/1-1, SPP1710 SCHW1719/7-1, ME1567/9-1/2, LI 984/3-1/2, INST 211/744-1, FUGG SCHW1719/5-1, and FI1655/3-1.

Subjects
Proteomics, 0301 basic medicine, 0106 biological sciences, Thioredoxin-Disulfide Reductase, [SDV]Life Sciences [q-bio], Citric Acid Cycle, Thioredoxin h, Glutathione reductase, Arabidopsis, Respiratory chain, seed germination, Germination, Reductase, Mitochondrion, 01 natural sciences, Redox, redox regulation, 03 medical and health sciences, Adenosine Triphosphate, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, Multidisciplinary, Arabidopsis Proteins, in vivo biosensing, Chemistry, Metabolism, Plants, Genetically Modified, redox proteomics, Cell biology, Oxygen, mitochondria, Citric acid cycle, Glutathione Reductase, 030104 developmental biology, Enzyme, PNAS Plus, Biochemistry, Mitochondrial matrix, Seeds, Oxidation-Reduction, 010606 plant biology & botany
Abstract

Seeds preserve a far developed plant embryo in a quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since the switchover from quiescence to reactivation provides a remarkable case of a cell physiological transition we investigated the earliest events in energy and redox metabolism of Arabidopsis seeds at imbibition. By developing fluorescent protein biosensing in intact seeds, we observed ATP accumulation and oxygen uptake within minutes, indicating rapid activation of mitochondrial respiration, which coincided with a sharp transition from an oxidizing to a more reducing thiol redox environment in the mitochondrial matrix. To identify individual operational protein thiol switches, we captured the fast release of metabolic quiescence in organello and devised quantitative iodoacetyl tandem mass tag (iodoTMT)-based thiol redox proteomics. The redox state across all Cys peptides was shifted toward reduction from 27.1% down to 13.0% oxidized thiol. A large number of Cys peptides (412) were redox switched, representing central pathways of mitochondrial energy metabolism, including the respiratory chain and each enzymatic step of the tricarboxylic acid (TCA) cycle. Active site Cys peptides of glutathione reductase 2, NADPH-thioredoxin reductase a/b, and thioredoxin-o1 showed the strongest responses. Germination of seeds lacking those redox proteins was associated with markedly enhanced respiration and deregulated TCA cycle dynamics suggesting decreased resource efficiency of energy metabolism. Germination in aged seeds was strongly impaired. We identify a global operation of thiol redox switches that is required for optimal usage of energy stores by the mitochondria to drive efficient germination.

Published
2020
Full Text
View/download PDF

5. A Versatile Workflow for the Identification of Protein–Protein Interactions Using GFP-Trap Beads and Mass Spectrometry-Based Label-Free Quantification

Author

Priyadarshini Tilak, Guillaume Née, and Iris Finkemeier

Subjects
0106 biological sciences, 0303 health sciences, Chemistry, fungi, Intact protein, Mass spectrometry, 01 natural sciences, Green fluorescent protein, Protein–protein interaction, 03 medical and health sciences, Label-free quantification, Biochemistry, Interaction network, In vivo, Protein Interaction Networks, 030304 developmental biology, 010606 plant biology & botany
Abstract

Protein functions often rely on protein-protein interactions. Hence, knowledge about the protein interaction network is essential for an understanding of protein functions and plant physiology. A major challenge of the postgenomic era is the mapping of protein-protein interaction networks. This chapter describes a mass spectrometry-based label-free quantification approach to identify in vivo protein interaction networks. The procedure starts with the extraction of intact protein complexes from transgenic plants expressing the protein of interest fused to a GFP-Tag (bait-GFP), as well as plants expressing a free GFP as background control. Enrichment of the GFP-tagged protein together with its interaction partners, as well as the free GFP, is performed by immunoaffinity purification. The pull-down quality can be evaluated by simple gel-based techniques. In parallel, the captured proteins are trypsin-digested and relatively quantified by label-free mass spectrometry-based quantification. The relative quantification approach largely relies on the normalization of protein abundances of background-binding proteins, which occur in both bait-GFP and free GFP pull-downs. Therefore, relative quantification of the protein pull-down is superior over methods that solely rely on protein identifications and removal of often copurified high-abundance proteins from the bait-GFP pull-downs, which might remove real interaction partners. A further strength of this method is that it can be applied to any soluble GFP-tagged protein.

Published
2020
Full Text
View/download PDF

6. Significance tests and statistical inequalities for region matching

Author

Guillaume Née, Marinette Revenu, Luc Brun, Stéphanie Jehan-Besson, General Electric Medical Systems [Buc] (GE Healthcare), General Electric Medical Systems, Equipe Image - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), and Normandie Université (NU)

Subjects
Matching (statistics), Similarity (geometry), business.industry, Pattern recognition, 02 engineering and technology, Decision rule, 01 natural sciences, 010104 statistics & probability, Motion estimation, Video tracking, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], Outlier, 0202 electrical engineering, electronic engineering, information engineering, 020201 artificial intelligence & image processing, Artificial intelligence, Noise (video), 0101 mathematics, business, Mathematics, Statistical hypothesis testing
Abstract

International audience; Region matching - finding conjugate regions on a pair of images - plays a fundamental role in computer vision. Indeed, such methods have numerous applications such as indexation, motion estimation or tracking. In the vast literature on the subject, several dissimilarity measures have been proposed in order to determine the true match for each region. In this paper, under statistical hypothesis of similarity, we provide an improved decision rule for patch matching based on significance tests and the statistical inequality of McDiarmid. The proposed decision rule allows to validate or not the similarity hypothesis and so to automatically detect matching outliers. The approach is applied to motion estimation and object tracking on noisy video sequences. Note that the proposed framework is robust against noise, avoids the use of statistical tests and may be related to the a contrario approach.

Published
2008
Full Text
View/download PDF

7. The release of dormancy, a wake-up call for seeds to germinate

Author

Wim J. J. Soppe, Yong Xiang, and Guillaume Née

Subjects
2. Zero hunger, 0106 biological sciences, 0301 basic medicine, biology, Seed dormancy, food and beverages, Germination, Plant Science, biology.organism_classification, Plant Dormancy, 01 natural sciences, 03 medical and health sciences, 030104 developmental biology, Seedling, Seedlings, Botany, Seeds, Dormancy, Imbibition, 010606 plant biology & botany
Abstract

Seed dormancy determines the timing of germination, thereby contributing to successful seedling establishment and plant fitness. The induction and release of dormancy are controlled by various regulators like plant hormones and dormancy proteins. The relative strengths of these regulators are influenced by environmental factors during seed maturation and storage. In the last few years additional processes have been identified to be involved in the release of dormancy during seed storage with an important role for non-enzymatic oxidative reactions. However, the relations between the different dormancy regulators are not fully understood yet. Finally, all accumulated information will be processed in the seed during early seed imbibition and lead to the decision to germinate or not.

Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results