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In this work, an exploratory study was conducted to examine Gram staining based on the capillary tube. Each Gram staining step for all bacterial strains tested was completed in capillary tubes. The results showed that different Gram staining morphologies were clearly visible in the capillary tubes. The results presented here demonstrated that the improved method could effectively distinguish between Gram-positive and Gram-negative bacteria, and only small volumes of reagents were required in this method. Collectively, this efficient method could rapidly and accurately identify the types of bacteria. Therefore, our findings could be used as a useful reference study for other staining methods.

A new heterometallic complex containing bis-Schiff base, namely, [(Salbn)NiNa(DMF)(H2O)]·N(CN)2 (1) (Salbn = bis(3-methoxysalicylidene) benzene-1, 2-diamine, DMF = N, N-dimethyl formamide) has been prepared through one-pot refluxing and condensation approach. Complex 1 was characterized by elemental analysis, IR spectroscopy, X-ray single-crystal diffraction, and powder X-ray diffraction analysis. The intermolecular hydrogen bonding interaction connects the Schiff base complex units through the [N(CN)2]− anion into dimer aggregate. These aggregates are further linked by weak hydrogen bondings and strong π–π interactions to give rise to one-dimensional chelating double-chain supramolecular polymer. Cyclic voltammetry measurement reveals the oxidation and reduction process of the compound is irreversible in nature. Polymer 1 exhibits emission spectrum maximum at 548 nm under ultraviolet excitation. Antibacterial property measurement indicates that 1 has strong inhibition toward Staphylococcus aureus.