1. Glycyrrhiza polysaccharide doped the conducting polymer PEDOT hybrid-modified biosensors for the ultrasensitive detection of microRNA
- Author
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Hao Wang, Ni Hui, Lili Yang, Zhi-Ling Song, and Haitao Lü
- Subjects
Biocompatibility ,Polymers ,Metal Nanoparticles ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Biofouling ,PEDOT:PSS ,Polysaccharides ,Glycyrrhiza ,Environmental Chemistry ,Spectroscopy ,Detection limit ,Conductive polymer ,Chemistry ,010401 analytical chemistry ,Reproducibility of Results ,Electrochemical Techniques ,Bridged Bicyclo Compounds, Heterocyclic ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,MicroRNAs ,Colloidal gold ,Gold ,0210 nano-technology ,Biosensor ,Protein adsorption - Abstract
Biosensors have tremendous revolutionary potential for the precise location and rapid detection of biomarkers for human disease. However, the minimization of nonspecific protein adsorption interactions and surface contamination is critical for their application in complex media. We report herein, the antifouling interface was constructed by electrochemical copolymerization of poly (3,4-ethylenedloxythlophene) (PEDOT) and glycyrrhiza polysaccharide (GPS). Hydrophilic PEDOT/GPS can reduce the interference and nonspecific adsorption of biological protein macromolecules, which has been verified by electrochemical and fluorescent characterization. Gold nanoparticles (AuNPs) were subsequently modified onto PEDOT/GPS surface to attach biomacromolecules containing thiol groups. MicroRNA, the promising biomarker of a large numbers of genetic diseases, was used as the testing model. Due to the robust antifouling capability of PEDOT/GPS as well as high biocompatibility of GPS/AuNPs, the fabricated biosensor based on PEDOT/GPS/AuNPs demonstrated excellent sensing performance, such as a wide detection range (0.01 nM–10 nM), a low detection limit (300 fM) and high reproducibility, showing great potential of medical applications.
- Published
- 2020