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10 results on '"Orestis Faklaris"'

1. Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants

Author

Edouard Bertrand, Nathalie Pochon, Eric Maréchal, Sahar Hani, James Whelan, Rémy Merret, Marie-Christine Thibaud, Laura Cuyas, Pascale David, Laurent Nussaume, Orestis Faklaris, Hélène Javot, David Secco, Florian Mueller, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Signalisation de l'Adaptation des Végétaux à l'Environnement (SAVE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Department of Animal Plant and Soil Sciences, La Trobe Institute for Agriculture and Food (LIAF), La Trobe University-La Trobe University, Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Imagerie et Modélisation - Imaging and Modeling, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biologie végétale et microbiologie environnementale - UMR7265 (BVME), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Centre Mondial de l'Innovation Roullier, Groupe Roullier, La Trobe University [Melbourne], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), LIPID, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Plant Environmental Physiology and Stress Signaling (PEPSS), S.H. was supported by a PhD fellowship from the CEA and PACA region, ANR Reglisse 13-906 ADAP-008 fellowship and CEA DRF impulsion program with FOSSI project supported EM, LC, LN, MCT, PD. Additional grant support were also received by HJ from CEA-Enhanced Eurotalent and ANR PhlowZ 19-CE-13-0007.We acknowledge the MRI imaging facility (belonging to the National Infrastructure France-BioImaging supported by the French National Research Agency, ANR-10-INBS-04),and the ZoOM platform (supported by the Région Provence Alpes Côte d’Azur, the Conseil General of Bouches du Rhône, the French Ministry of Research, the Centre National de la Recherche Scientifique and the Commissariat à l’Energie Atomique et aux Energies Alternatives)., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-13-ADAP-0008,Reglisse,Régulation de l'interconversion des glycérolipides chez les plantes en réponse aux variations environnementales(2013), and ANR-19-CE13-0007,PHLOWZ,Approche systémique de l'interaction entre les voies de signalisation du Zinc et du Phosphate(2019)

Subjects
0106 biological sciences, Transcription, Genetic, [SDV]Life Sciences [q-bio], Cell, Arabidopsis, RNA polymerase II, Plant Science, 01 natural sciences, Phosphates, 03 medical and health sciences, Gene Expression Regulation, Plant, Stress, Physiological, Plant Cells, medicine, Transcriptional regulation, Allele, Gene, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, biology, RNA, biology.organism_classification, Cell biology, Kinetics, medicine.anatomical_structure, biology.protein, RNA Polymerase II, Steady state (chemistry), 010606 plant biology & botany
Abstract

International audience; Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, in real time and under changing environmental conditions. Using phosphate-responsive genes as a model, we found that active genes displayed high transcription initiation rates (one initiation event every ~3 s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpectedly fast kinetics (on the order of minutes) and striking heterogeneity between neighbouring cells. Access to single-cell RNA polymerase II dynamics in live plants will benefit future studies of signalling processes.

Published
2021

2. Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues

Author

Tkhe Kyong Fam, Thierry Galli, Andrey S. Klymchenko, Pichandi Ashokkumar, Lydia Danglot, Mayeul Collot, Orestis Faklaris, Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Biophotonique et Pharmacologie (CNRS UMR 7213), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))

Subjects
Models, Molecular, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Indoles, multicolor imaging, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Catalysis, KB Cells, chemistry.chemical_compound, Mice, Colloid and Surface Chemistry, Lipid droplet, Microscopy, Organelle, Moiety, Animals, Humans, Merocyanine, Benzopyrans, Fluorescent Dyes, tissue imaging, communication, Chemistry, Optical Imaging, Nile red, dioxaborine, General Chemistry, Lipid Droplets, 021001 nanoscience & nanotechnology, Fluorescence, Two-Photon Excitation imaging, 0104 chemical sciences, Mice, Inbred C57BL, Adipose Tissue, Liver, Microscopy, Fluorescence, Biophysics, 0210 nano-technology, Intracellular
Abstract

Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes, and cancers. Herein we present a family of new fluorogenic merocyanine fluorophores based on an indolenine moiety and a dioxaborine barbiturate derivative. These so-called StatoMerocyanines (SMCy) fluoresce from yellow to the near-infrared (NIR) in oil with an impressive fluorescence enhancement compared to aqueous media. Additionally, SMCy display remarkably high molar extinction coefficients (up to 390 000 M–1 cm–1) and high quantum yield values (up to 100%). All the members of this new family specifically stain the LDs in live cells with very low background noise. Unlike Nile Red, a well-known lipid droplet marker, SMCy dyes possess narrow absorption and emission bands in the visible, thus allowing multicolor imaging. SMCy proved to be compatible with fixation and le...

Published
2018

3. Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening

Author

Orestis Faklaris, Jurriaan M. Zwier, Thierry Durroux, Martin Cottet, Eric Trinquet, and Jean-Philippe Pin

Subjects
Drug, media_common.quotation_subject, lcsh:Medicine, lcsh:RS1-441, Pharmaceutical Science, Review, Computational biology, Biology, Bioinformatics, 01 natural sciences, lcsh:Pharmacy and materia medica, 03 medical and health sciences, GPCR, Drug Discovery, lanthanides, 030304 developmental biology, media_common, G protein-coupled receptor, 0303 health sciences, lcsh:R, Binding properties, time resolved FRET, Screening assay, Ligand (biochemistry), Fluorescence, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Molecular Medicine, fluorescence, Heterologous expression, screening assay
Abstract

The identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement constitute excellent alternatives to the traditional radioactive assays. Less hazardous, faster and cheaper, these methods also exhibit very good sensitivity and can be used on various biological models such as heterologous expression systems or native tissues.

Published
2011

4. Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells

Author

Jean-Paul Boudou, Damien Garrot, Patrick A. Curmi, Thierry Sauvage, Orestis Faklaris, Fran c cois Treussart, Vandana Joshi, Laboratoire de Photonique Quantique et Moléculaire (LPQM), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de mécanique des sols, structures et matériaux (MSSMat), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec, Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), Financement européen: 'Nano4Drugs' (contrat LSHB-2005-CT-019102), Financement région 'Ile de France'(C'Nano IdF): projet 'Biodiam'., NaDia (contrat ANR-2007-PNANO-045),NaDia (contrat ANR-2007-PNANO-045), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-07-NANO-0045,NADIA,Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant(2007), CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Treussart, François, and Nanosciences et nanotechnologies - Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant - - NADIA2007 - ANR-07-NANO-0045 - NANO - VALID

Subjects
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], Photoluminescence, Materials science, Fluorophore, FOS: Physical sciences, Nanoparticle, quantum dots, 02 engineering and technology, 010402 general chemistry, photostability, 01 natural sciences, chemistry.chemical_compound, [PHYS.QPHY]Physics [physics]/Quantum Physics [quant-ph], Molecule, [PHYS.QPHY] Physics [physics]/Quantum Physics [quant-ph], Condensed Matter - Materials Science, Quantum Physics, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], business.industry, Materials Science (cond-mat.mtrl-sci), 021001 nanoscience & nanotechnology, Photobleaching, [PHYS.COND.CM-MS] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Atomic and Molecular Physics, and Optics, 0104 chemical sciences, chemistry, Quantum dot, nanodiamonds, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Optoelectronics, Particle, biomarker, photoluminescence, Quantum Physics (quant-ph), 0210 nano-technology, business, Excitation, Optics (physics.optics), Physics - Optics
Abstract

International audience; Long-term observations of photoluminescence at the single-molecule level were until recently very difficult, due to the photobleachingof organic fluorophore molecules. Although inorganic semiconductor nanocrystals can overcome this difficulty showing very low photobleachingyield, they suffer from photoblinking. A new marker has been recently introduced, relying on diamond nanoparticles containingphotoluminescent color centers. In this work we compare the photoluminescence of single quantum dots (QDs) to the one of nanodiamondscontaining a single-color center. Contrary to other markers, photoluminescent nanodiamonds present a perfect photostability andno photoblinking. At saturation of their excitation, nanodiamonds photoluminescence intensity with a single NV-color center is only threetimes smaller than one of the single QDs. Moreover, the bright and stable photoluminescence of nanodiamonds allows wide field observationsof single nanoparticles motion. We demonstrate the possibility of recording the trajectory of such single particle in culture cells.

Published
2009

5. Diamond nanoparticles as photoluminescent nanoprobes for biology and near-field optics

Author

François Treussart, Damien Garrot, Orestis Faklaris, Aurélien Cuche, Jean-François Roch, Thierry Sauvage, Yannick Sonnefraud, Jean-Paul Boudou, Serge Huant, Nano-Optique et Forces (NOF), Institut Néel (NEEL), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Biogéochimie et écologie des milieux continentaux (Bioemco), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Nano-Optique et Forces (NEEL - NOF), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), NOF - Nano-Optique et Forces, Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), and Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Photoluminescence, Biophysics, Physics::Optics, Nanoparticle, FOS: Physical sciences, Nanotechnology, 02 engineering and technology, 01 natural sciences, Biochemistry, law.invention, 010309 optics, Optical microscope, law, fluorescent probes, 0103 physical sciences, Condensed Matter - Materials Science, Near-field optics, Materials Science (cond-mat.mtrl-sci), nitrogen-vacancy (NV) colour centres, General Chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Atomic and Molecular Physics, and Optics, near-field scanning optical microscopy, 3. Good health, PACS: 78.67.Bf, 07.79.Fc, 33.50.- j, nanodiamonds, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Near-field scanning optical microscope, Diamond nanoparticles, 0210 nano-technology, active tips
Abstract

We present results showing the potential of diamond nanoparticles with size less than 50 nm as photoluminescent nanoprobes for serving as stable point-like emitters attached at the tip apex of a near-field optical microscope to achieve enhanced spatial resolution., Comment: Keynote talk by F. Treussart at the 15th Int. Conf. on Luminescence and Optical Spectroscopy of Condensed Matter (ICL 08), Lyon, July 2008. Proceedings to appear in J. Luminescence

Published
2009

6. Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells

Author

Alain Thorel, Theano Irinopoulou, Hugues A. Girard, Jean-Paul Boudou, Céline Gesset, Patrick A. Curmi, François Treussart, Patrick Tauc, Mohamed Senour, Jean-Charles Arnault, Orestis Faklaris, Vandana Joshi, Faklaris, Orestis, Nanosciences et nanotechnologies - Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant - - NADIA2007 - ANR-07-NANO-0045 - NANO - VALID, Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre des Matériaux (CDM), Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), 'Nano4Drugs' (contract LSHB-2005-CT-019102), ANR-07-NANO-0045,NADIA,Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant(2007), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Centre des Matériaux (MAT), Centre National de la Recherche Scientifique (CNRS)-PSL Research University (PSL)-MINES ParisTech - École nationale supérieure des mines de Paris, 'NaDia' (contract ANR-2007-PNANO-045),'NaDia' (contract ANR-2007-PNANO-045), and MINES ParisTech - École nationale supérieure des mines de Paris

Subjects
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], Macromolecular Substances, Surface Properties, Molecular Conformation, FOS: Physical sciences, General Physics and Astronomy, Nanoparticle, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, Clathrin, diamond, Materials Testing, Humans, Nanotechnology, endocytosis, General Materials Science, Particle Size, chemistry.chemical_classification, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Staining and Labeling, biology, Vesicle, Biomolecule, General Engineering, Colocalization, biolabel, 021001 nanoscience & nanotechnology, Nanostructures, 0104 chemical sciences, Endocytic vesicle, chemistry, Transmission electron microscopy, Luminescent Measurements, biology.protein, Biophysics, nanoparticles, photoluminescence, Crystallization, 0210 nano-technology, Optics (physics.optics), Physics - Optics, HeLa Cells
Abstract

International audience; Diamond nanoparticles (nanodiamonds) have been recently proposed as new labels for cellular imaging. For small nanodiamonds (size

Published
2009

7. Detection of single photoluminescent diamond nanoparticles in cell and study of the internalization pathway

Author

Orestis Faklaris, Vandana Joshi, Patrick Georges, François Treussart, Jean-Paul Boudou, Frédéric Druon, Patrick A. Curmi, Thierry Sauvage, Damien Garrot, Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Charles Fabry de l'Institut d'Optique / Elsa, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), Biogéochimie et écologie des milieux continentaux (Bioemco), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-07-NANO-0045,NADIA,Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant(2007), European Project, Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Treussart, François, Nanosciences et nanotechnologies - Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant - - NADIA2007 - ANR-07-NANO-0045 - NANO - VALID, Nano4Drugs : contrat LSHB-2005-CT-019102 - INCOMING, Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), NaDia : contrat ANR-2007-PNANO-045,NaDia : contrat ANR-2007-PNANO-045, Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)

Subjects
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], Luminescence, Materials science, Photochemistry, Endosome, media_common.quotation_subject, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Fluorescent Antibody Technique, Nanoparticle, Nanotechnology, Endosomes, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, law.invention, Biomaterials, diamond, Confocal microscopy, law, Humans, General Materials Science, Internalization, ComputingMilieux_MISCELLANEOUS, media_common, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Colocalization, General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Autofluorescence, Drug delivery, cytotoxicity, biomarker, nanoparticles, photoluminescence, 0210 nano-technology, Intracellular, HeLa Cells, Biotechnology
Abstract

International audience; Diamond nanoparticles are promising photoluminescent probes for tracking intracellular processes, due to embedded perfectly photostable color centers. In this work, we investigate the spontaneous internalization of such nanoparticles (diameter 25 nm) in HeLa cells by confocal microscopy and time-resolved techniques. Nanoparticles are observed inside the cell cytoplasm at the single particle and single color center level, assessed by time correlation intensity measurements. Improvement of the nanoparticles signal-to-noise ratio inside cell is achieved using a pulsed excitation laser and a time resolved detection taking advantage of the long radiative lifetime of the color center excited state, compared to cell auto-fluorescence. The internalization pathways are also investigated, with endosomal marking and colocalization analyses. The low colocalization ratio observed proves that nanodiamonds are not trapped in endosomes, a promising result in prospect of drug delivery by these nanoparticles. We also prove a low cytotoxicity of these nanoparticles in this cell line. Submitted to-2

Published
2008

8. Diamond nanoparticles as photoluminescent nanoprobes

Author

Thierry Sauvage, Orestis Faklaris, Jean-Paul Boudou, Vandana Joshi, Patrick A. Curmi, François Treussart, Serge Huant, Aurélien Cuche, Jean-François Roch, Yannick Sonnefraud, Laboratoire de Photonique Quantique et Moléculaire (LPQM), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), NOF - Nano-Optique et Forces, Institut Néel (NEEL), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Centre d'Études et de Recherches par Irradiation (CERI), Centre National de la Recherche Scientifique (CNRS), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biogéochimie et écologie des milieux continentaux (Bioemco), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Nano-Optique et Forces (NOF), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Nano-Optique et Forces (NEEL - NOF), École normale supérieure - Paris (ENS-PSL), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)

Subjects
Photoluminescence, General Physics and Astronomy, markers, Nanotechnology, 02 engineering and technology, Living cell, engineering.material, 01 natural sciences, law.invention, color centers, Optical microscope, diamond, law, near-field optics, 0103 physical sciences, 010302 applied physics, Physics, chemistry.chemical_classification, Biomolecule, Near-field optics, Diamond, 021001 nanoscience & nanotechnology, chemistry, [PHYS.COND.CM-GEN]Physics [physics]/Condensed Matter [cond-mat]/Other [cond-mat.other], engineering, Diamond nanoparticles, 0210 nano-technology
Abstract

Proceedings of COLOQ'10 - 10ème COlloque sur les Lasers et l'Optique Quantique, Grenoble, July 2007; We present preliminary results showing the potential of diamond nanoparticles with size

Published
2007

9. Quenching nitrogen–vacancy center photoluminescence with an infrared pulsed laser

Author

V. Jacques, Ngoc Diep Lai, Dingwei Zheng, François Treussart, Huan-Cheng Chang, Orestis Faklaris, Jean-François Roch, Laboratoire de Photonique Quantique et Moléculaire (LPQM), and École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Photoluminescence, Infrared, FOS: Physical sciences, General Physics and Astronomy, 02 engineering and technology, engineering.material, 01 natural sciences, [PHYS.QPHY]Physics [physics]/Quantum Physics [quant-ph], 0103 physical sciences, 010306 general physics, Absorption (electromagnetic radiation), ComputingMilieux_MISCELLANEOUS, Quenching, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], business.industry, Diamond, 021001 nanoscience & nanotechnology, Nanocrystal, Picosecond, engineering, Optoelectronics, 0210 nano-technology, business, Nitrogen-vacancy center, Optics (physics.optics), Physics - Optics
Abstract

Diamond nanocrystals containing Nitrogen-Vacancy (NV) color centers have been used in recent years as fluorescent probes for near-field and cellular imaging. In this work we report that an infrared (IR) pulsed excitation beam can quench the photoluminescence of NV color center in a diamond nanocrystal (size < 50 nm) with an extinction ratio as high as ~90%. We attribute this effect to the heating of the nanocrystal consecutive to multi-photon absorption by the diamond matrix. This quenching is reversible: the photoluminescence intensity goes back to its original value when the IR laser beam is turned off, with a typical response time of hundred picoseconds, allowing for a fast control of NV color center photoluminescence. We used this effect to achieve sub-diffraction limited imaging of fluorescent diamond nanocrystals on a coverglass. For that, as in Ground State Depletion super-resolution technique, we combined the green excitation laser beam with the control IR depleting one after shaping its intensity profile in a doughnut form, so that the emission comes only from the sub-wavelength size central part., 5 figures, 15 pages

Published
2013

10. Diamond nanocrystals hosting single nitrogen-vacancy color centers sorted by photon-correlation near-field microscopy

Author

Yannick Sonnefraud, Thierry Sauvage, François Treussart, Jean-François Roch, Orestis Faklaris, Serge Huant, Aurélien Cuche, and Jean-Paul Boudou

Subjects
Optics and Photonics, Time Factors, Materials science, Photoluminescence, Nitrogen, Annealing (metallurgy), Molecular Probe Techniques, 02 engineering and technology, engineering.material, 01 natural sciences, 010309 optics, Materials Testing, 0103 physical sciences, Microscopy, Nanotechnology, Particle Size, Nanodiamond, Photons, business.industry, Resolution (electron density), Diamond, Equipment Design, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, Nanostructures, Nanocrystal, engineering, Nanoparticles, Optoelectronics, Near-field scanning optical microscope, Crystallization, 0210 nano-technology, business
Abstract

Diamond nanocrystals containing highly photoluminescent color centers are attractive, nonclassical, and near-field light sources. For near-field applications, the size of the nanocrystal is crucial, since it defines the optical resolution. Nitrogen-vacancy (NV) color centers are efficiently created by proton irradiation and annealing of a nanodiamond powder. Using near-field microscopy and photon statistics measurements, we show that nanodiamonds with sizes down to 25 nm can hold a single NV color center with bright and stable photoluminescence.

Published
2008

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