1. Toward a harmonized and standardized protocol for the determination of total hydroxytyrosol and tyrosol content in virgin olive oil (VOO). The pros of a fit for the purpose ultra high performance liquid chromatography (UHPLC) procedure
- Author
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Bojan Butinar, Ole Winkelmann, Stefania Vichi, Diego L. García-González, Maurizio Servili, Tullia Gallina Toschi, Aspasia Mastralexi, Maria Z. Tsimidou, Nikolaos Nenadis, European Commission, Tsimidou M.Z., Nenadis N., Mastralexi A., Servili M., Butinar B., Vichi S., Winkelmann O., Garcia-Gonzalez D.L., and Toschi T.G.
- Subjects
Química dels aliments ,LC-HRMS ,Cost effectiveness ,Proton Magnetic Resonance Spectroscopy ,Pharmaceutical Science ,HPLC-DAD ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Drug Discovery ,Virgin olive oil ,Sample preparation ,Hydroxytyrosol ,Chromatography, High Pressure Liquid ,Mathematics ,Health claim ,04 agricultural and veterinary sciences ,Phenylethyl Alcohol ,Reference Standards ,040401 food science ,Oli d'oliva ,Chemistry (miscellaneous) ,European Commission Regulation 432/2012 ,Molecular Medicine ,Food composition ,Ultra high performance ,Fenols ,1H-NMR spectroscopy ,Article ,lcsh:QD241-441 ,0404 agricultural biotechnology ,lcsh:Organic chemistry ,Phenols ,Tyrosol ,Physical and Theoretical Chemistry ,Olive Oil ,Protocol (science) ,Chromatography ,Elution ,010401 analytical chemistry ,Organic Chemistry ,Normalització ,UHPLC-DAD ,Standardization ,0104 chemical sciences ,chemistry ,Olive oil - Abstract
&Tau, oward a harmonized and standardized procedure for the determination of total hydroxytyrosol and tyrosol content in virgin olive oil (VOO), the pros of a recently published in house validated ultra high performance liquid chromatography (UHPLC) protocol are discussed comparatively with those of other procedures that determine directly or indirectly the compounds hosted under the health claim on &ldquo, olive oil polyphenols&rdquo, (EC regulation 432/2012). Authentic VOOs were analyzed with five different liquid chromatographic separation protocols and 1H-NMR one in five different laboratories with expertise in VOO phenol analysis within three months. Data comparison indicated differences in absolute values. Method comparison using appropriate tools (Passing-Bablok regression and Bland Altman analyses) for all protocols vs. the UHPLC one indicated slight or statistically significant differences. The results were also discussed in terms of cost effectiveness, detection means, standard requirements and ways to calculate the total hydroxytyrosol and tyrosol content. Findings point out that the in-house validated fit for the purpose UHPLC protocol presents certain pros that should be exploited by the interested parties. These are the simplicity of sample preparation, fast elution time that increase the number of samples analyzed per day and integration of well-resolved peaks with the aid of only two commercially available external standards. Importance of correction factors in the calculations is stressed.
- Published
- 2019