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Brown stripe is a major fungal disease damaging the leaves of sugarcane. Breeding and cultivating resistant varieties is the most economical and effective method of controlling the disease. The paper reports on the evaluation of sugarcane brown stripe resistance in novel varieties bred by the China Sugarcane System and major varieties under cultivation in the sugarcane cultivating region. Field resistance against brown stripe was identified in 60 novel varieties and in 31 major varieties under cultivation in Lincang, Puer, and Yuxi, in Yunnan Province, and in Yizhou, Guangxi Province, where the incidence and severity of brown stripe is particularly high. Field surveys showed that 32 (53.3%) of the 60 novel varieties were highly resistant to moderately resistant, and 28 (46.7%) were susceptible to highly susceptible. In addition 21 (67.7%) out of the 31 major cultivated varieties were highly resistant to moderately resistant, and 10 (32.3%) were susceptible to highly susceptible. We recommend the intensifying of efforts to eliminate the major varieties that are susceptible, and promotion of the adoption of elite resistant varieties in wet and rainy sugarcane cultivating regions with high incidence and severity of sugarcane brown stripe disease, to effectively manage the brown stripe epidemic.

Sugarcane brown rust, caused by Puccinia melanocephala, is one of the main diseases of sugarcane in China. The identification and discovery of new resistance genes have important theoretical and practical significance for preventing outbreaks of brown rust and ensuring the sustainable production of sugarcane. To screen for polymorphic simple-sequence repeat (SSR) molecular markers for localization of brown rust resistance genes, we used two populations that are suitable for genetic linkage map construction and mapping of new resistance genes to construct resistant and susceptible genetic pools. We then screened 449 pairs of primers to identify polymorphic SSR markers in the parental lines and the resistant/susceptible genetic pools. The results showed that 25 pairs of primers directed amplification of polymorphic DNA fragments between the parents of the cross combination ‘Yuetang 03-393’ × ‘ROC 24’, and 16 pairs of primers amplified polymorphic fragments between the parents of the cross combination ‘Liucheng 03-1137’ × ‘Dezhe 93-88’. Four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636 and SCESSR2551) amplified polymorphic DNA fragments between the parental lines and the resistant/susceptible genetic pools in ‘Yuetang 03-393’ × ‘ROC 24’. The results of this study will establish a solid foundation for the mapping of new brown rust resistance genes, genetic linkage map construction and the development of closely-associated molecular markers in sugarcane.

Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Exploring novel resistance genes and breeding varieties with durable resistance is the most economical and effective way of controlling brown rust. The establishment of complete and high-density molecular genetics maps is a key prerequisite for the discovery and location of sugarcane brown rust resistance genes. To obtain more polymorphic simple sequence repeat (SSR) markers for the construction of genetic linkage maps, six highly resistant varieties and six highly susceptible varieties were used as parents to screen the primers with clear bands, obvious polymorphism, and good repeatability. The highest numbers of polymorphic primers were obtained from the parental group of Liucheng 03-1137 × Dezhe 93-88 (52.38%). Ten pairs of primers including mSSCIR34 exhibited high polymorphism in the parental group of Liucheng 03-1137 × Dezhe 93-88. Here, we recommend ten SSR pairs of polymorphic primers including mSSCIR34 to use in the mapping populations of Liucheng 03-1137 × Dezhe 93-88. The polymorphic primers could facilitate the construction of genetic maps and lay an appropriate foundation for the localization of brown rust resistance genes and development of molecular genetics linkage markers.

We assessed inheritance of resistance to sugarcane brown rust (Puccinia melanocephala) in selfing F1 populations of wild sugarcane germplasm Erianthus rockii ‘Yundian 95-19’ and E. rockii ‘Yundian 95-20’. We tested parent and selfing F1 individuals for the brown rust resistance gene, Bru1, that has been shown to confer resistance to brown rust in sugarcane. The Bru1 gene was not detected in E. rockii ‘Yundian 95-19’, E. rockii ‘Yundian 95-20’ or their selfing F1 individuals, and we found there was segregation of resistance in the two selfing F1 populations (segregation ratio: 3:1). The results confirmed resistance in E. rockii ‘Yundian 95-19’ and E. rockii ‘Yundian 95-20’ to sugarcane brown rust is controlled by a novel, single dominant gene.

Sugarcane leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is an extremely destructive disease. In 2017, suspected symptoms of sugarcane leaf scald was observed, in Baoshan, Mengding and Jinping, Yunnan Province, China. The bacteria was isolated from the symptomatic stalks and was subsequently identified as the causal agent using Koch’s postulates. BLASTN analysis showed that the amplified nucleotide sequences of 16S rRNA, gyrB and rpoD genes from all isolates shared 100% sequence identity with the nucleotide sequences of corresponding genes of X. albilineans strain GPE PC73 (Acc no. FP565176). Based on symptom diagnosis in the field, colony morphology, validation of Koch’s postulates and molecular identification of 16S rRNA, gyrB and rpoD genes, the disease was confirmed as sugarcane leaf scald caused by X. albilineans. Partial 16S rRNA, gyrB and rpoD gene sequence analysis of 36 X. albilineans strains from Yunnan and Guangxi provinces, China, was determined. The results revealed that the gyrB gene was the most sensitive in differentiating X. albilineans strains from Yunnan and Guangxi provinces.

Significance Our article presents the most comprehensive reconstruction of the evolutionary and phylogeographic history of a major plant pathogen of brassica vegetables in Eurasia. Sampling across such a large landmass poses considerable challenges, and our study attempts to describe the spatial and temporal patterns of migration for a plant pathogen on a large scale. Our phylogeographic and molecular clock analyses show that the migration pathways of turnip mosaic potyvirus retrace some of the historical trade arteries of the Silk Road. This study demonstrates how a comprehensive genetic analysis can provide a large-scale view of the epidemiology and human-mediated spread of a plant pathogen across centuries of evolutionary history., Plant pathogens have agricultural impacts on a global scale and resolving the timing and route of their spread can aid crop protection and inform control strategies. However, the evolutionary and phylogeographic history of plant pathogens in Eurasia remains largely unknown because of the difficulties in sampling across such a large landmass. Here, we show that turnip mosaic potyvirus (TuMV), a significant pathogen of brassica crops, spread from west to east across Eurasia from about the 17th century CE. We used a Bayesian phylogenetic approach to analyze 579 whole genome sequences and up to 713 partial sequences of TuMV, including 122 previously unknown genome sequences from isolates that we collected over the past five decades. Our phylogeographic and molecular clock analyses showed that TuMV isolates of the Asian-Brassica/Raphanus (BR) and basal-BR groups and world-Brassica3 (B3) subgroup spread from the center of emergence to the rest of Eurasia in relation to the host plants grown in each country. The migration pathways of TuMV have retraced some of the major historical trade arteries in Eurasia, a network that formed the Silk Road, and the regional variation of the virus is partly characterized by different type patterns of recombinants. Our study presents a complex and detailed picture of the timescale and major transmission routes of an important plant pathogen.

Sugarcane white leaf (SCWL) is a devastating sugarcane (Saccharum officinarum) disease caused by a 16SrXI group phytoplasma, which is extremely harmful to sugarcane production. To determine the occurrence of SCWL in different varieties in 2018, we conducted a field survey and performed nested PCR detection of SCWL phytoplasma in cane-planting areas of Mangweng and Hepai in Gengma, Yunnan province, which are the areas most severely affected by SCWL in China. The results of the field survey showed that the symptomatic incidence of SCWL differed among varieties. The mean symptomatic incidence of SCWL on variety Yuetang60 was the highest (73.50%), and it was the lowest on Liucheng05-136 (13.67%). Using nested PCR, the SCWL phytoplasma was detected in symptomatic plants of all varieties more than 90% of the time; the SCWL phytoplasma was detected in 91 and 97% of symptomatic plants of Yingyu91-59 and Liucheng05-136 varieties, respectively. The SCWL phytoplasma was detected by PCR in 82% of the asymptomatic plant samples. The results of this study showed that field survey based on white leaf symptoms did not accurately reflect the actual occurrence of the SCWL phytoplasma.

Sugarcane mosaic disease is one of the most serious and prevalent viral diseases of sugarcane in China. Resistant varieties are the most economical and effective measures for controlling this disease. Resistance against Sugarcane streak mosaic virus (SCSMV) and Sorghum mosaic virus (SrMV) in 41 excellent sugarcane innovation germplasms and their parents (Saccharum officinarum L. “Ludashi” × Erianthus rockii Keng “Yundian 95-19”) was checked by using a stalk inoculation and RT-PCR detection during 2015 and 2016. Results indicated that among the 41 excellent sugarcane innovation germplasms and parents, 23 were highly (Grade 1) to moderately resistant (Grade 3) to SCSMV, meanwhile, 31 were highly (Grade 1) to moderately resistant (Grade 3) to SrMV. Ten germplasms were highly resistant (Grade 1) to resistant (Grade 2) to both SCSMV and SrMV. Among these, six germplasms (Yun 09-604, Yun 09-607, Yun 09-619, Yun 09-633, Yun 09-656, Yundian 95-19) were highly resistant (Grade 1) to both SCSMV and SrMV, accounting for 13.95% of the total germplasm materials. These results may provide resistance resource available for the breeding program of sugarcane cultivars against the major vrius associated with mosaic disease in China.

Field resistance against sugarcane thrips (Fulmekiola serratus) in 21 sugarcane varieties was tested following inoculation with a natural population. The results revealed differences in pest incidence levels in different varieties and between different leaf positions in the same variety. The tested varieties were subsequently categorized into four levels of resistance (Grades 1–4). The variety GT31 and LC05-136 showed the highest resistance (Grade 1), with a pest index of less than 0.35, while 12 varieties including FN91-21 and FN39 had a pest index of 0.35–0.45 (Grade 2). Five varieties including YT60 and YZ03-194 had a pest index of 0.45–0.55 (Grade 3), while FN38 and DZ03-83 had the lowest resistance (Grade 4), with a pest index of greater than 0.65. Correlation analysis further revealed that the number of thrips present on a single plant was related to the level of resistance; however, the correlation was relatively weak. These findings provide a preliminary evaluation of thrips resistance screening in sugarcane, laying the foundation for analysis of thrips-resistant genes, breeding of thrips-resistant varieties and resistance evaluation in new sugarcane germplasms.

Sugarcane mosaic disease is one of the most serious and prevalent viral diseases of sugarcane in China. The use of resistant varieties is the most economical and effective measures for controlling this disease. Sugarcane streak mosaic virus (SCSMV) and Sorghum mosaic virus (SrMV) are the two predominant pathogens of mosaic disease in the cane-growing regions of China. In 2015, 2016, double resistance to SCSMV and SrMV was identified once a year using a combination of artificial stalk-cutting inoculation and RT-PCR detection in 71 new elite sugarcane varieties/clones. Of the 71 new elite sugarcane varieties/clones, 24 were highly (Grade 1) to moderately resistant (Grade 3), and 47 were susceptible (Grade 4) to highly susceptible (Grade 5) to SCSMV. Furthermore, 27 were highly (Grade 1) to moderately resistant (Grade 3), and 44 were susceptible (Grade 4) to highly susceptible (Grade 5) to SrMV. Fifteen new elite sugarcane varieties/clones were highly resistant (Grade 1) to resistant (Grade 2) to SCSMV and SrMV. While, five new elite sugarcane varieties/clones (Yuegan 34, Yuetang 55, Yunzhe 03–258, Yunzhe 05–51, Yunzhe 06–80) were highly resistant (Grade 1) to SCSMV and SrMV. We defined the resistance of 71 new elite sugarcane varieties/clones to the two main pathogens of mosaic disease and identified 15 resistant to both SCSMV and SrMV. These results provide an elite resistance resource for effective prevention and control of mosaic disease and may serve as a reference for commercial varieties.

Brown rust, caused by Puccinia melanocephala has become a serious problem for sugarcane production in China. Therefore, 50 new elite varieties bred by the Chinese Sugarcane System were screened for brown rust resistance and the presence of a major resistance gene, Bru 1. Identification of field resistance and molecular detection of the brown rust resistance gene Bru 1 in the 50 new elite varieties and 2 main cultivated varieties were carried out in 2014 and 2015 at regional experimental stations of the China Sugarcane System in Dehong and Baoshan, Yunnan Province, where the incidence of brown rust is particularly high. Resistance evaluations based on ratings of symptom severity resulting from field infection showed that 32 (64%) of the 50 new elite varieties were highly resistant (n = 13, 26%), resistant (n = 16, 32%) and moderately resistant (n = 3, 6%). Molecular detection identified Bru 1 in 27 (54%) of these resistant varieties suggesting that brown rust resistance in these new elite varieties is primarily controlled by Bru 1. The absence of Bru 1 in the remaining five resistant suggested the presence of additional brown rust resistance genes. Two main cultivated varieties were highly resistant and Bru 1 - positive varieties. Different series differed in the frequencies of resistant and Bru 1 - positive varieties. Yunzhe series varieties had the highest frequency of resistant varieties (87.5%) and the highest frequency of Bru 1-positive varieties (81.3%); In contrast, the Yuetang series varieties had the lowest frequency of resistant varieties (40%) and the lowest frequency of Bru 1-positive varieties (30%). In this study, we defined the field resistance of 50 new elite varieties to brown rust, and determined the distribution of brown rust resistant gene Bru 1 in different series varieties. These results will provide new options for commercial production with effective resistance to brown rust. Moreover, these new elite resistant varieties can be used for subsequent breeding and selection to produce agronomically superior varieties with effective brown rust resistance.

Brown rust caused by Puccinia melanocephala H. Sydow & P. Sydo is an important sugarcane disease that severely threatens sugarcane production in China. Breeding and planting resistant cultivars is the most cost-effective and efficient strategy for the control the disease. In addition, exploration, research, and utilization of resistant genes are the basis for resistance breeding. In the present study, four sugarcane varieties without the Bru1 gene and highly resistant to brown rust were used as male parents, and four varieties highly susceptible to brown rust were used as the female parents to configure hybrids. Six F1 hybrid populations were subjected to authenticity determination using SSR markers, brown rust resistance phenotype identification using artificial inoculation, and molecular detection of Bru1 gene. The results showed that the genetic segregation ratios in F1 hybrid populations of Yuetang 03-393 (HS) × ROC24 (HR) and Liucheng 03-1137 (HS) × Dezhe 93-88 (HR) were 3R:1S and 1R:3S, respectively, and Bru1 gene was not detected in their F1 hybrids. The results indicated that two dominant novel genes controlled brown rust resistance in Yuetang 03-393 (HS) × ROC24 (HR) and two recessive novel genes controlled brown rust resistance in Liucheng 03-1137 (HS) × Dezhe 93-88 (HR). Two genetically segregating populations that could be used for the localization of novel brown rust resistance genes were developed in the present study, and could lay a suitable foundation and provide theoretical reference for the analysis of the genetic mechanism of brown rust resistance, genetic map construction, mapping of novel resistance genes and the development of closely associated molecular markers in the future.

In 2015, symptoms of red stripe were first viewed on leaves of sugarcane plants growing in Yuanjiang, Yunnan Province. Symptomatic leaf samples were collected from infected plants and isolations were made on NA medium. Pure culture isolates from resulting single colony were identified based on cultural and morphological characteristics as Acidovorax avenae subsp. avenae. Morphological identification was confirmed by sequence analysis of 16S rDNA. Pathogenicity tests confirmed Yunnan isolates of Acidovorax avenae subsp. avenae as the causal agent of red stripe on sugarcane. This is the first report of this disease in Yuanjiang, Yunnan, China. Field surveys showed that the sugarcane cultivar ‘Yunzhe03-194’ was highly susceptible, and disease incidence varied from 8 to 80%. Nine other sugarcane cultivars were found to be symptomless in the field. Further research is required to confirm the resistance levels of the sugarcane varieties.

Seventy-seven mosaic leaf samples from new sugarcane varieties from national regional trials in Kaiyuan, Mile and Yuanjiang, Yunnan Province, China, were collected in 2015 and analyzed using reverse-transcription polymerase chain reaction (RT-PCR) to determine the pathogen causing mosaic symptoms in sugarcane. Three viruses, Sugarcane streak mosaic virus (SCSMV), Sorghum mosaic virus (SrMV) and Sugarcane mosaic virus (SCMV), were detected in 100, 27.3 and 1.3% of the samples respectively. Co-infection with SCSMV, SrMV and SCMV was also found for the first time in the new variety Qiantang 5. Nucleotide sequences of the coat protein (CP) gene of 48 SCSMV and 17 SrMV isolates were subsequently sequenced and analyzed with the CP gene sequences of SCSMV and SrMV reported in GenBank to perform phylogenetic analyses. Geographic differences were observed in the phylogenetic tree of SCSMV and all SCSMV isolates from China clustered into a single China group. Moreover, SCSMV isolates from different new varieties were distributed across all subgroups or clustered into an independent branch in the China group. SrMV isolates obtained in this study and those published in GenBank clustered into two groups and were distributed into different subgroups within these groups. Furthermore, the distribution of SrMV isolates from different new varieties overlapped in these different groups and subgroups, with no obvious geographic differences. These results provide a scientific basis for sugarcane mosaic or streak mosaic disease resistance breeding and effective control of this disease.

Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Bru1 is a major brown rust resistance gene and has been shown to provide broad-spectrum resistance against various brown rust pathogen strains originating from different countries. Thus, brown rust resistance genes have important implications for sugarcane brown rust resistance breeding programs. The objectives of this study were to (1) assess the level of resistance of common sugarcane crossbreeding parents to P. melanocephala, (2) determine the distribution of the Bru1 gene in common sugarcane crossbreeding parents in China, (3) screen, evaluate, and select rust-resistant parental lines to improve the efficiency of breeding for brown rust resistance. Brown rust resistance in 101 common sugarcane breeding parents was collected from the Chinese National Nursery of Sugarcane Germplasm Resources and was scored and identified at the seedling stage and was assayed for the presence of the brown rust resistance gene Bru1. The results showed that Bru1 was detected in 48 (47.5 %) resistant clones out of the 101 tested parental lines, indicating that brown rust resistance in common sugarcane breeding parents in China is primarily controlled by Bru1. The apparent absence of Bru1 from the other 29 resistant and 24 susceptible parental lines suggested that they may carry brown rust resistance-associated genes other than Bru1. These results may facilitate future efforts to breed varieties with improved resistance to brown rust and may provide additional resistant parental lines for selecting and planting elite-resistant varieties to effectively control brown rust disease in sugarcane.

Sugar cane white leaf (SCWL) is a serious disease caused by phytoplasmas. In this study, we performed nested PCR with phytoplasma universal primer pairs (P1/P7 and R16F2n/R16R2) for the 16S rRNA gene to detect SCWL phytoplasmas in 31 SCWL samples collected from Baoshan and Lincang, Yunnan, China. We cloned and sequenced the nested PCR products, revealing that the 16S rRNA gene sequences from 31 SCWL samples were all 1247 bp in length and shared more than 99 % nucleotide sequence similarity with the 16S rRNA gene sequences of SCWL phytoplasmas from various countries. Based on the reported 16S rRNA gene sequence data from SCWL isolates of various countries, we conducted phylogenetic and virtual RFLP analysis. In the resulting phylogenetic tree, all SCWL isolates clustered into two branches, with the Lincang and Baoshan SCWL phytoplasma isolates belonging to different branches. The virtual RFLP patterns show that phytoplasmas of the Lincang branch belong to subgroup 16SrXI-B. However, the virtual RFLP patterns revealed by HaeIII digestion of phytoplasmas of the Baoshan branch differed from those of subgroup 16SrXI-B. According to the results of phylogenetic and virtual RFLP analysis, we propose that the phytoplasmas of the Baoshan branch represent a new subgroup, 16SrXI-D. These findings suggest that SCWL is caused by phytoplasmas from group 16SrXI, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D.

For many years, the control and treatment of sugarcane pests in China mainly relies on highly toxic and broad-spectrum chemical pesticides, such as methamidophos and omethoate. As a result, many nontargets, natural enemy species rapid declines, the dynamic relationship between pests and their natural enemies were disrupted, and pests develop resistance, natural enemy population decreased. It alleviates the function on natural factor of controlling pests and makes the prevention and control work more passive. Moreover, the misuse of pesticides leads to pesticide residue, environment pollution, and human health harmfulness. Therefore, a new task for plant protection workers is to control the pests in a comprehensive and environmentally friendly way. In recent years, biological control has become one of the important measures in integrated pest control, and its social and ecological benefits are attracting more and more attention from the state and society (Pu 1984; Bao 1988; Pu and Li 1996; Zhou et al. 1999). The study and development of using dominant natural enemies to control the pests can get the obvious effect on increasing yield, reducing environmental pollution, maintaining ecological balance, saving energy, and cutting down production cost (Yang et al. 2006; An et al. 2009). With China’s accession to the WTO, it is becoming increasingly important to protect and utilize the natural enemies of sugarcane pests, when the implementation of the process of quality standardization of agricultural products has been continuously promoted.

There are more than 120 sugarcane diseases that have been found in the world presently (Chen 1982; Rott et al. 2000), and more than 60 have been reported in China (The important sugarcane diseases research cooperation group 1991; Lu et al. 1997; Huang and Li 2014, 2016). Mastering the disease types, damage, and distribution in the sugarcane area can provide scientific basis for the sugarcane disease-resistance breeding, quarantine of introduced varieties, and disease management and research. Sugarcane production areas in China have drought in winter and spring and hot climate and good rainfall in summer and autumn. With the rise of temperature in spring, in case of cold current as well as the rainfall and temperature decline and the poor management, planted sugarcane in winter and this season are easily infected by pineapple disease and the ratoon crops are easily damaged by red rot disease. Because the whole seedling stage is in the dry season, smut becomes substantial causing sparse breakup. After June, with the high temperature and humidity, eyespot, brown stripe, yellow spot, pokkah boeng, rust, ring spot, and leaf scorch diseases are easily occurring and epidemic. Especially in recent years, introducing seedcanes frequently and transposing seedcanes in sugarcane planting area make some dangerous seedcane-transmitted diseases (ratoon stunting disease, mosaic, smut, yellow leaf syndrome, etc.) which are spread by seedcane between sugarcane areas. Then, caused sugarcane diseases occur commonly and damage more severely; have a serious impact on sugarcane yield, quality, and ratooning capacity; and bring serious hidden trouble to the safe production of sugarcane. In order to scientifically control the spread and damage of sugarcane disease, enhancing the capabilities of disaster prevention and reduction and ensuring the quality of sugarcane variety and safety of sugarcane production in 20 diseases that commonly occur in sugarcane production are systematically described in this chapter with clear-colored photos and scientific, accurate words. The content includes the occurrence and damage, identification of symptoms, characteristics of epidemic infection, and control measures.

Using chemical herbicides to replace artificial and mechanical weeding in the field is a widely used technology worldwide. It represents low-cost prevention and control, saves working hours, reduces labor intensity, and can effectively prevent weeds in a timely manner, thus ensuring normal growth of sugarcane plants for high yield (Su and Song 1996). Improper use of chemical herbicides can cause phytotoxicity, which leads to abnormal sugarcane normal growth and yield reductions. The correct use of herbicides, based on the weed species and scientific spraying according to the soil moisture content, can be effective to control weeds in sugarcane fields. This chapter uses clear color photos and scientific, accurate text to describe systematically the main weeds of sugarcane fields and their distribution, growth and decline law in the field, and chemical weed control measure.

Leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is a destructive disease of sugarcane, as it causes severe economic losses (Rott et al. 1995). X. albilineans has been reported in over 60 countries in Africa, Asia, and North and South Americas (Rott et al. 2000), but has so far not been reported in mainland China. X. albilineans is a quarantine pathogen for sugarcane germplasm imported into China. In June 2016, symptoms of leaf scald were observed on the newly-bred sugarcane cultivars ‘Guitang46’ and ‘Guitang06-2081’ in Beihai, Laibin and Baise, Guangxi province, China. The average disease incidence of cultivars ‘Guitang46’ and ‘Guitang06-2081’ was 44.6% and 50.1%, respectively. Typical symptoms of the disease were white stripes along veins. Severely infected plants also exhibited shorter stalk internodes, smaller leaves, and abnormal development of side shoots along the stalk. Leaves of side shoots had similar white stripes as those observed at the top of the stalk. A sudden wilting of all lea...