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Biological monitoring and assessment are the first and most fundamental steps towards diagnosing ecological or environmental quality. Increasing anthropogenic impact on urban ecosystems has prompted the development of less expensive and more efficient bioassessment approaches. Generally, a morphospecies based approach is effective for plants and large organisms but challenging for the microbial biosphere. To overcome this challenge, we used high-throughput DNA sequencing for predicting anthropogenic effects on microeukaryotic communities in urban waterbodies along a pollution gradient in Wuhan City, central China in summer 2019. Our results indicated that microeukaryotic community structure was distinct between non-urban polluted reservoir and urban polluted waterbodies. The heterogeneity of environmental condition significantly affected the microeukaryotic diversity, community structure, and species interactions. Integrated co-occurring network analysis revealed that the pollution gradient has a significant adverse impact on network complexity and network dissimilarity. These results revealed that the significant variation in anthropogenically-driven environmental condition shaped microeukaryotic communities in urban freshwater ecosystems. Furthermore, we observed that the relative abundance of indicative OTUs were significantly and negatively correlated with pollution level and these indicative OTUs could be used to predict the water quality status with up to 77% success. Thus, our multiple approaches combining 18S rDNA amplicon sequencing, co-occurring network and indicator species analyses suggest that this study gives a novel approach based on microeukaryotic communities to assess and predict the water quality status of urban aquatic environments.

Fish passage facilities are constructed to enable fish to pass anthropogenic barriers such as dams though their efficiency varies across species and location. There are a number of studies that assess the efficiency of fish passage facilities, yet rarely have such assessments been conducted in Asia. We conducted one of the first quantitative assessments of the efficiency of a vertical-slot fishway in Asia on the Daduhe River in Southwest China. Quantitative assessment of fish passage efficiency was conducted using a combination of methods, including fish sampling, video recordings and a Passive Integrated Transponder (PIT) system for tracking individually-tagged fish (N = 69 of 6 species). Fish sampling revealed 40 species assembled downstream of the dam. Fish captured closer to the fishway tended to be larger than fish caught more distant from the fishway. Half of the fish species observed downstream of the fishway were also observed at the entrance to the fishway (i.e. 153 individuals across 20 species). Video records revealed that overall passage rates were 71.2% based on the number of fish observed at the exit of the viewing chamber relative to that observed passing the entrance viewing chamber. Most fish passed the fishway at night with peak passage occurring in June. PIT technology results revealed that passage efficiency among the six tagged species ranged from 0% to 60% (four species successfully ascended the fishway). Transit time from the fishway entrance to exit was variable both among and within species that successfully ascended the fishway (i.e. 17.9–20.3 h for Schizothorax davidi, 6.4–88.8 h for Schizothorax preuanti, 46.4 h for Silurus meridionalis, 22.1–53.9 h Semilabeo prochilus). Fishway performance varied by species such that there is evidence that the fishway may be useful for maintaining river connectivity for some species. However, passage was often restricted during periods when there was sufficient flow in the fishway. Additional research is needed to put these findings in an ecological context given the overall low number of fish that passed the dam.