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Soybean (Glycine max [L.] Merr.) is a food and oil crop whose growth and yield are influenced by root and nodule development. In the present study, GmNMHC5 was found to promote the formation of nodules in overexpressing mutants. In contrast, the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply. In 35S:GmNMHC5 mutants, expression levels of genes involved in nodulation were significantly up-regulated. Both in vitro and in vivo biochemical analyses showed that GmNMHC5 directly interacted with GmGAI (a DELLA protein), and the content of gibberellin 3 (GA3) in overexpressing mutants was lower than that in the wild type. These results revealed that GmNMHC5 participates in the classical GA signaling pathway, and may regulate the content of GA3 to match the optimal concentration required for nodule formation, thereby promoting nodulation by directly interacting with GmGAI. A model illustrating the mechanism by which GmNMHC5 promotes soybean nodulation is presented.

The intrinsic carbon defects including pentagons, vacancies and sp3-hybridized carbon have recently been proposed as efficient reactive sites for oxygen reduction reaction (ORR). Nevertheless, it is still a great challenge to controllably introduce the intrinsic defects into carbon materials. Herein, a universal defect-engineering method via vaporized salt is reported to modify the N-doped carbon surface with abundant sp3-hybridized carbon defects. At an elevated temperature, the vaporized sodium chloride is found to selectively modulate the surface structure of carbon material. The obtained carbon-based electrocatalyst delivers an outstanding electrocatalytic ORR property with a half-wave potential (E1/2) of 0.85 V vs. RHE and an excellent performance in zinc air battery (ZAB) test. The analysis of components and structures of surface elements via XANES and XPS reveals that the increasing sp3-hybridized carbon defects, induced by the vaporized-salt modification, are responsible for the enhancement of ORR performance. The theoretical calculations further suggest the sp3 component hybridizes with original sp2 carbon, forming efficient sp2/sp3 hybridized carbon sites towards ORR. Additionally, other halide salts are proved to have the similar effect on promoting ORR activity and this method can expand to other carbon-based materials, suggesting its universality and significance in synthesis of defect-rich carbon-based materials.

Quantifying the influences of land use/cover (LULC) change on hydrological processes is important for rational utilization of water resources. The objective of this study was to evaluate the impacts of spatiotemporal LULC change on hydrological components in a typical agricultural area located in the North China Plain at both basin and sub-basin scales. LULC change was quantified, and the Soil and Water Assessment Tool was optimized using parameters associated with LULC conditions. We concluded that the urban and forest areas increased by 25.57 and 10.56%, with the cropland area decreased by 36.76%. About half of the surface runoff (SURQ) in the basin was generated from the urban area, with the SURQ increased significantly in the upstream and downstream of the basin where overlapped with urbanized areas. The proportions of evapotranspiration generated by cropland and forest areas increased slightly (0.89 and 0.55%, respectively), especially in sub-basins where the conversion of cropland to forest was obvious. Urban, forest, and cropland were the main types that generated water yield (WYLD). The proportion of WYLD generated on the urban area increased by 9.55% and decreased in other areas, which may be related to the combined effects of urbanization and forest reduction. Highlights The SWAT model was used under different scenarios for a typical agricultural area, North China Plain.; The influences of land use/cover change on key hydrological components were analyzed at both basin and sub-basin scales.; Urbanization and reforestation will affect changes in hydrological components.

The reverse projection protocol results in fast phase-contrast imaging thanks to its compatibility with conventional computed-tomography scanning. Many researchers have proposed variants. However, all these reverse projection methods in grating-based phase-contrast imaging are built on the hypothesis of the synchronous phase of reference shifting curves in the whole field of view. The hypothesis imposes uniformity and alignment requirements on the gratings, thus the field of view is generally limited. In this paper, a generalized reverse projection method is presented analytically for the case of non-uniform reference in grating-based phase tomography. The method is demonstrated by theoretical derivation, numerical simulations and synchrotron radiation experiments. The influence of imaging position to sensitivity, and the phase-wrapping phenomenon are also discussed. The proposed method combines the advantages of the high efficiency of the reverse projection method and the universal applicability of the phase-stepping method. The authors believe that the method would be used widely in fast and dose-constrained imaging.

Developing an efficient catalyst for hydrogen (H2) generation from hydrolysis of ammonia borane (AB) to significantly improve the activity for the hydrogen generation from AB is important for its practical application. Herein, we report a novel hybrid nanostructure composed of uniformly dispersed Co@Co2P core-shell nanoparticles (NPs) embedded in N-doped carbon nanotube polyhedron (Co@Co2P/N–CNP) through a carbonization-phosphidation strategy derived from ZIF-67. Benefiting from the electronic effect of P doping, high dispersibility and strong interfacial interaction between Co@Co2P and N-CNTs, the Co@Co2P/N–CNP catalyst exhibits excellent catalytic performance towards the hydrolysis of AB for hydrogen generation, affording a high TOF value of 18.4 mol H2 mol metal−1 min−1 at the first cycle. This work provides a promising lead for the design of efficient heterogeneous catalysts towards convenient H2 generation from hydrogen-rich substrates in the close future.

Anthropogenic aerosol (AA) forcing has been shown as a critical driver of climate change over Asia since the mid-20th century. Here we show that almost all Coupled Model Intercomparison Project Phase 6 (CMIP6) models fail to capture the observed dipole pattern of aerosol optical depth (AOD) trends over Asia during 2006–2014, last decade of CMIP6 historical simulation, due to an opposite trend over eastern China compared with observations. The incorrect AOD trend over China is attributed to problematic AA emissions adopted by CMIP6. There are obvious differences in simulated regional aerosol radiative forcing and temperature responses over Asia when using two different emissions inventories (one adopted by CMIP6; the other from Peking university, a more trustworthy inventory) to driving a global aerosol-climate model separately. We further show that some widely adopted CMIP6 pathways (after 2015) also significantly underestimate the more recent decline in AA emissions over China. These flaws may bring about errors to the CMIP6-based regional climate attribution over Asia for the last two decades and projection for the next few decades, previously anticipated to inform a wide range of impact analysis.

Nitrogen fixation in legumes requires the development of specialized organs called root nodules. Here we characterized the high-confidence transcriptome and genome-wide patterns of H3K4me3 marks in soybean roots and mature nodules symbiotic with Sinorhizobium fredii. Changes in H3K4me3 levels were positively associated with the transcription levels of functional genes in the nodules. The up-regulation of H3K4me3 levels was not only present in leghaemoglobin and nodulin-related genes, but also in genes involved in nitrogen and carbon metabolic pathways. In addition, genes regulating the transmembrane transport of metal ions, phosphates, sulphates, peptides, and sugars were differentially modified. On the contrary, a loss of H3K4me3 marks was found in several key transcription factor genes and was correlated with the down-regulation of the defense-related network in nodules, which could contribute to nodule maintenance. All these findings demonstrate massive reprogramming of gene expressions via alterations in H3K4me3 levels in the genes in mature soybean nodules.

This study examines the mechanisms by which the East Asian summer monsoon (EASM) changes in response to non–East Asian (NEA) anthropogenic aerosol forcing by distinguishing the fast direct atmospheric response and slow ocean-mediated response to forcing using a global aerosol–climate coupled model. The results show that NEA aerosol forcing significantly exacerbates the weakening of the EASM due to local aerosol forcing. The fast response is dominant in the weakening of the EASM and an anomalous precipitation pattern over eastern China resembling the “southern flood and northern drought” pattern in the total response to NEA aerosol forcing. Changes in upper-tropospheric temperature caused by the fast response play a major role in the impact of NEA aerosol forcing on the EASM. Anomalous cooling occurs during summer in the upper troposphere (at ~40°N) over East Asia caused by the fast response. This is due to the combined effects of strong eastward cold advection in the Northern Hemisphere midlatitudes caused by increased aerosol loading in Europe and the resulting change in local meridional heat transport in East Asia. Subsequently, the zonal wind speed changes on either side of the anomalous cooling, and the East Asian subtropical jet shifts equatorward, thereby weakening the EASM. The changes in atmospheric temperature and the local Hadley cell caused by the slow response to NEA aerosol forcing are conducive to strengthening the southwesterly winds over eastern China. Our study suggests the importance of NEA aerosol forcing in driving changes in the EASM on a fast time scale.

Circulating tumor cells (CTCs) are an important part of liquid biopsy as they represent a potentially rich source of information for cancer diagnosis, monitoring, prognosis, and treatment guidance. It has been proved that the nanotopography interaction between cells and the surface of CTC detection platforms can significantly improve the capture efficiency of CTCs, whereas many mature nanostructure substrates have been developed based on chemistry materials. In this work, a natural biointerface with unique biological properties is fabricated for efficient isolation and nondestructive release of CTCs from blood samples using the cancer cell membranes. The cell membrane interfaces are proved to have a good antiadhesion property for nonspecific cells because of their own electronegativity. A natural surface nanostructure is provided by the cancer cell membrane to nicely match with the surface nanotopography of CTCs. Bovine serum albumin (BSA) as a linker and DNA aptamer against the epithelial cell adhesion molecule (EpCAM) as a specific affinity molecule are then introduced onto the cell membrane interfaces to achieve the highly efficient and specific capture of CTCs. Finally, the captured target cells can be intactly released from the substrate using the complementary DNA sequence with controlling the incubation time. This study provides a smart strategy in the development of a natural biological interface for the isolation and release of CTCs with high purity.

One of the key challenges with atomic catalysts (single, double, and triple atom catalysts) is the relatively low loading of active materials, resulting in the low mass or volume activity of catalysts, which hinders their rapid development in the field of catalysis. Herein, using density functional theory (DFT), we explore a series of atomic catalysts with heterostructured substrate, graphdiyne and graphene (GDY/Gra), for the nitrogen reduction reaction (NRR). The theoretical mass loading of active metal atoms can exceed 35.0 wt% in the triple atom catalyst (TAC), which could be reached through the optimal substrate and suitable precursors in experiments. Among the designed atomic catalysts, Fe3-GDY/Gra with a theoretical mass loading of 35.8 wt% shows outstanding catalytic activity for the electrocatalytic NRR with a potential of −0.26 V vs. the reversible hydrogen electrode (RHE). Moreover, the proposed TACs show better catalytic activity for the NRR than single atom catalysts (SACs) and double atom catalysts (DACs) due to the unique properties of M3 (M = Mn, Fe, Co, and Ni) active sites, which provide more electrons for activating N2, but also have a weak adsorption to more easily release products. These findings provide a new strategy for designing atomic catalysts with high loading, high stability, and high activity.

Orthogonal and single-factor experiments were used to investigate the remediation effect of single and mixed washing on arsenic-contaminated soil from a typical non-ferrous metal smelter. The optim...

It has been commonly accepted that soybean domestication originated in East Asia. Although East Asia has the historical merit in soybean production, the USA has become the top soybean producer in the world since 1950s. Following that, Brazil and Argentina have been the major soybean producers since 1970s and 1990s, respectively. China has once been the exporter of soybean to Japan before 1990s, yet she became a net soybean importer as Japan and the Republic of Korea do. Furthermore, the soybean yield per unit area in East Asia has stagnated during the past decade. To improve soybean production and enhance food security in these East Asian countries, much investment has been made, especially in the breeding of better performing soybean germplasms. As a result, China, Japan, and the Republic of Korea have become three important centers for soybean genomic research. With new technologies, the rate and precision of the identification of important genomic loci associated with desired traits from germplasm collections or mutants have increased significantly. Genome editing on soybean is also becoming more established. The year 2019 marked a new era for crop genome editing in the commercialization of the first genome-edited plant product, which is a high-oleic-acid soybean oil. In this review, we have summarized the latest developments in soybean breeding technologies and the remarkable progress in soybean breeding-related research in China, Japan, and the Republic of Korea.

To evaluate urban flood risk while considering spatial and temporal characteristics, this paper establishes an assessment method based on a coupled hydrodynamic model and a fuzzy comprehensive evaluation method. An assessment system is developed using hydrologic, social and economic factors. In addition, hydrology factors are obtained by the coupled hydrodynamic model. The hydrodynamic model is solved using the three-cascade method of the Saint–Venant equations for a river system, and the surface runoff is solved using a modified Roe-type approximate Riemann solver. The hydrology results are used as hydrologic factors and directly reflect the inundation of the subsequent assessment. Afterward, an analytic hierarchy process is used to calculate the weights of the assessment indices. Then, the urban flood risk is analyzed using the fuzzy comprehensive evaluation method. Five different rainfall events in Quzhou City are simulated and evaluated. The numerically computed results compare well with the statistical data and indicate an extreme inundation depth of more than 3 m. In addition, the assessment risk of Quzhou City is at level IV (high). This study shows that combining the assessment method with the coupled hydrodynamic model efficiently reflects the urban flood risk by considering flood, social and economic data.

The method for detection of circulating tumor cells (CTCs) is considered as a promising cancer liquid biopsy technique. However, due to the rarity and heterogeneity, it is still a challenging task to capture CTC with high purity and viability from blood for the following enumeration and molecular analysis. Herein, we fabricated a hydrogel-chitosan nanofiber substrate to effectively capture the target cancer cells, assisted by the poly(carboxybetaine methacrylate) (pCBMA) hydrogel and the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody. The pCBMA hydrogel layer was fabricated by free radical polymerization to provide an antifouling surface, which could help to reduce the non-specific capture of blood cells to improve the capture purity of CTC. Then, chitosan nanofibers were introduced onto the hydrogel layer to provide a nanostructured surface to improve the capture efficiency. Finally, the capture yield of artificial blood samples on the anti-EpCAM modified hydrogel-nanofiber substrate was up to 79.9%, revealing the potential application of the hydrogel-nanofiber substrate on efficient and sensitive capture of rare circulating tumor cells.

The recent development in plasmon-enhanced electrochemical hydrogen production provides an alternative approach to address the bottleneck of low hydrogen production efficiency in the conventional photocatalysis. To achieve high-efficiency plasmonic catalysis, the development of catalytically active Schottky heterostructure is essential. Herein, we propose a 3D bicontinuous nanoporous plasmonic heterostructure for hydrogen evolution reaction (HER) by growing HER-active monolayer MoS2 on the internal surfaces of plasmonic 3D nanoporous gold (MoS2@NPG). The catalytically active plasmonic heterostructure with a large specific surface area and high electric conductivity exhibits superior photoelectrochemical activities towards electrochemical hydrogen production at both low onset potential of −70 mV vs RHE and the Tafel slope of 38 mV dec−1 under visible light illumination. The outstanding HER performance can be attributed to the efficient transfer of plasmon-generated hot electrons from NPG to monolayer MoS2 as well as the effective electron–hole separation by the Schottky barriers at MoS2/NPG interfaces.

The accuracies of the two-stream discrete-ordinate-method (DOM) and Eddington approximation schemes are systematically compared using the BCC_RAD radiative transfer scheme used in a general circulation model (GCM). It is found that the two-stream DOM produces more accurate results for the upward radiative flux, downward radiative flux and heating rate under clear-sky conditions in an offline radiation model, whereas the Eddington approximation is more accurate under all-sky conditions. An experiment using satellite data as the approximation of cloud properties confirms the superiority of the Eddington approximation under cloudy-sky conditions. Experiments using the GCM of the Beijing Climate Center (BCC_AGCM2.0.1) show that, compared to the two-stream DOM, the Eddington approximation can enhance the fraction of low cloud, and this increased cloud fraction can affect the differences in radiative fluxes between these schemes. This study suggests that the more suitable approach in GCMs is to use the Eddington approximation.

With the acceleration of globalization, trade between countries has become more and more frequent; as a kind of commonly used heavy lifting equipment, the influence of the quayside crane on environment during the production stage has attracted wide attention. In this paper, the world’s largest quayside crane manufacturer was selected as the study object. The environmental load of five workshops (NC pre-treatment workshop, assembling workshop, sand milling workshop, painting workshop and steel structure workshop) involved in the quayside crane (QC) production stage was studied by using the ReCiPe model; the normalized values of the 14 overall pollutants in each workshop were 1248.64, 576.75, 214.27, 85.26 and 76.69, respectively. The results showed that the sand milling workshop was the biggest electricity consumer of all processes. The sensitivity analysis revealed that the environmental pollution load was the most sensitive to the electricity consumption; the scenario analysis indicated that with the proportion of China’s renewable resources in the process of electricity generation becoming larger and larger, in comparison with that of in 2015, up to 2050, the quantity of PM 2.5 generated during the production process of QC will drop from 832 to 408 kg (high-speed development)/342 kg (low-speed development), and it is expected that the China’s haze problem could be effectively curbed in the future. Finally, the research results of this article also pointed out the direction for the future technical innovation of the QC industry.

Localized surface plasmon resonance (LSPR) excitation enhanced chemical and electrochemical reactions represent a promising pathway for solar-to-chemical energy conversion. However, plasmonic catalysts usually suffer from low collection efficiency of hot charge carries generated by LSPR excitation due to short lifetime of hot carriers and fast electron-hole recombination. Schottky barriers between plasmonic catalysts and semiconducting supports have been utilized to prevent the electron-hole recombination. However, the interfacial barriers also hinder low-energy hot charge collection and thus affect solar-to-chemical energy conversion efficiency. Here we report that bicontinuous nanoporous gold as a Schottky barriers-free direct plasmonic catalyst can significantly enhance electro-oxidation of alcohol molecules. A high energetic hole yield of 0.486% is achieved, which is 4 times higher than that of discrete plasmonic AuAg nanoparticles. The direct plasmonic catalyst offers the highest methanol oxidation current density of 531 µA cm−2 among all known Au catalysts. This work provides compelling evidence that higher hot carrier collection efficiency can be achieved from direct plasmonic electrocatalysis without the assistance of Schottky junctions and has important implications in developing high efficiency plasmonic catalysts for photo-enhanced electrochemical reactions.

ChIP-seq is widely used for mapping the transcription factor (TF) binding sites throughout the genome in vivo. In this study, we adopted and modified ChIPmentation, a fast, robust, low-input requirement ChIP-seq method, to a transient expression system using soybean protoplasts to expedite the exploration of TF binding sites. To test this new protocol, we expressed a tagged version of a C2H2-type zinc finger TF, JAGGED1 (GmJAG1), in soybean protoplasts and successfully identified its binding sites in the soybean genome. Furthermore, valuable genomic features such as a novel GmJAG1-binding motif, and the epigenetic characteristics as well as an enhancer-like function of GmJBSs were also found via coupling ATAC-seq and H3K27me3 ChIP-seq data. The application of the modified ChIPmentation protocol in this study using soybean protoplasts provided a new approach for rapid elucidation of how a TF binds to the various target genes in the soybean genome, as illustrated here using GmJAG1.

To mitigate the projected global warming in the 21st century, it is well-recognized that society needs to cut CO2 emissions and other short-lived warming agents aggressively. However, to stabilize the climate at a warming level closer to the present day, such as the “well below 2 ∘C” aspiration in the Paris Agreement, a net-zero carbon emission by 2050 is still insufficient. The recent IPCC special report calls for a massive scheme to extract CO2 directly from the atmosphere, in addition to decarbonization, to reach negative net emissions at the mid-century mark. Another ambitious proposal is solar-radiation-based geoengineering schemes, including injecting sulfur gas into the stratosphere. Despite being in public debate for years, these two leading geoengineering schemes have not been directly compared under a consistent analytical framework using global climate models. Here we present the first explicit analysis of the hydroclimate impacts of these two geoengineering approaches using two recently available large-ensemble (>10 members) model experiments conducted by a family of state-of-the-art Earth system models. The CO2-based mitigation simulation is designed to include both emission cuts and carbon capture. The solar-radiation-based mitigation simulation is designed to inject sulfur gas strategically at specified altitudes and latitudes and run a feedback control algorithm to avoid common problems previously identified such as the overcooling of the tropics and large-scale precipitation shifts. Our analysis focuses on the projected aridity conditions over the Americas in the 21st century in detailed terms of the potential mitigation benefits, the temporal evolution, the spatial distribution (within North and South America), the relative efficiency, and the physical mechanisms. We show that sulfur injection, in contrast to previous notions of leading to excessive terrestrial drying (in terms of precipitation reduction) while offsetting the global mean greenhouse gas (GHG) warming, will instead mitigate the projected drying tendency under RCP8.5. The surface energy balance change induced by sulfur injection, in addition to the well-known response in temperature and precipitation, plays a crucial role in determining the overall terrestrial hydroclimate response. However, when normalized by the same amount of avoided global warming in these simulations, sulfur injection is less effective in curbing the worsening trend of regional land aridity in the Americas under RCP8.5 when compared with carbon capture. Temporally, the climate benefit of sulfur injection will emerge more quickly, even when both schemes are hypothetically started in the same year of 2020. Spatially, both schemes are effective in curbing the drying trend over North America. However, for South America, the sulfur injection scheme is particularly more effective for the sub-Amazon region (southern Brazil), while the carbon capture scheme is more effective for the Amazon region. We conclude that despite the apparent limitations (such as an inability to address ocean acidification) and potential side effects (such as changes to the ozone layer), innovative means of sulfur injection should continue to be explored as a potential low-cost option in the climate solution toolbox, complementing other mitigation approaches such as emission cuts and carbon capture (Cao et al., 2017). Our results demonstrate the urgent need for multi-model comparison studies and detailed regional assessments in other parts of the world.

The soybean (Glycine max (L.) Merr.) is an important oil and food crop. Its growth and development is regulated by complex genetic networks, and there are still many genes with unknown functions in regulation pathways. In this study, GmNMHC5, a member of the MADS-box protein family, was found to promote flowering and maturity in the soybean. Gene expression profiling in transgenic plants confirmed that the 35S:GmNMHC5 T3 generation had early flowering and precocity. We used CRISPR-Cas9 to edit GmNMHC5 and found that late flowering and maturity occurred in Gmnmhc5 lines with stable inheritance. Remarkably, in the 35S:GmNMHC5 plants, the expression of flowering inhibitors GmFT1a and GmFT4 was inhibited. In addition, overexpression of GmNMHC5 in ft-10 (a late flowering Arabidopsis thaliana mutant lacking Flowering Locus T (FT) function) rescued the extremely late-flowering phenotype of the mutant A. thaliana. These results suggest that GmNMHC5 is a positive transcription factor of flowering and maturity in the soybean, which has a close relationship with FT homologs in the flowering regulation pathway. This discovery provides new ideas for the improvement of the flowering regulation network, and can also provide guidance for future breeding work.

The bubbles in the liquid film are important to improve the chemical reaction rate and the efficiency of heat and mass transfer. However, bubbles in annular flow have not been studied intensively like waves on film surface or droplets. Planar Laser-induced Fluorescence (PLIF) was employed to investigate the film bubbles in vertical upward annular flow. The subject of this paper is to study the correlation between the area, the gas holdup of bubbles in the liquid film and gas-liquid superficial velocity. The results show that the above parameters decrease with the increase of the gas superficial velocity, however, with the increase of liquid superficial velocity they are not always increasing. The variation of these parameters with the liquid superficial velocity is also diverse under different gas superficial velocity. The statistical characteristics of bubble area are studied and it can reveal how the mean bubble area decreases as the flow conditions change.

Falling film is widely applied in many industries, such as evaporation, condensation, chemical reaction and distillation. In order to investigate the influence of the liquid temperature on the wave characteristics in the free-falling film that is subjected to the sensible heating, instantaneous measurements of the film thickness using planar laser-induced fluorescence are performed. Experiments are carried out at liquid temperature that ranges from 24 °C to 52 °C. The spatiotemporal flow structure of falling film is reconstructed and analyzed at different temperatures. It is found that the frequency and the amplitudes of the large waves change with the temperature simultaneously, and the substrate regions become more smoother and wider with the temperature rising. The profiles of film thickness probability density function and the power spectrum density function are presented and discussed.

Aptamers have become the most promising recognition reagents in terms of early diagnosis and effective treatment of cancers. In this study, using cervical cancer as a model, we have identified a DNA aptamer specifically binding to cervical cancer cells with high affinity using the cell-SELEX (systematic evolution of ligands by exponential enrichment) method, in which a negative selection was carried out using normal epithelial cells as control. The binding abilities of 6 selected truncated aptamers were determined by laser confocal fluorescence microscopy and flow cytometry, while most of them only recognize the target cells and do not bind the control cells, and the aptamer C-9S with 51-mer shows the best binding affinity to Ca Ski cells (target cells) with a dissociation constant value of 19.3 ± 2.9 nM. Moreover, at physiological temperature, C-9S remains its specific recognition capability to Ca Ski cells as well. Meanwhile, C-9S shows a similar binding ability to another cervical cancer cells (HeLa). Therefore, on the basis of its excellent targeting properties and inherent functional versatility of aptamer, C-9S holds great potential to be a molecular probe for early detection, in vivo imaging, and targeted delivery for further researches in cancer.

Circulating tumor cells (CTCs) have been considered as a significant biomarker for cancer metastasis and relapse. Effective detection of CTCs will play an important role in early cancer diagnosis, prognosis, progress monitoring, and personalized therapy. In this work, we developed a polystyrene (PS) nanosphere substrate to capture CTCs from whole blood samples. Bovine serum albumin (BSA), as an antifouling molecule, and folic acid, as a recognizing agent, were modified onto the surface of PS nanospheres via self-polymerization of dopamine. 8 HeLa cells could be captured when 10 cells were spiked into 1 mL of the whole blood sample. Then, the captured target cells were further proliferated through an in situ culture strategy on the polystyrene (PS) nanosphere substrate, and nearly 80 HeLa cells were obtained from 1 captured cell after 7 days of in situ culture, revealing the potential application for CTC isolation and proliferation.

Three-dimensional porous materials with bicontinuous open porosity represent a new class of functional materials for various applications. Top-down dealloying has been demonstrated to be one of the most effective ways to fabricate 3D porous materials. Vapor phase dealloying, which makes use of the saturated vapor pressure difference between the constituent components in an alloy for selectively removing a less stable element or phase, is a promising versatile method for fabricating porous materials from active metals to inorganic elements. Here, using nickel-zinc and germanium-zinc alloys as the prototypes of single-phase and two-phase precursors, respectively, we report the fabrication of 3D bicontinuous porous Ni and Ge by vapor phase dealloying on the basis of selective element or selective phase evaporations. We also show the incorporation of vapor phase dealloying with chemical vapor deposition for the one-pot growth of 3D nanoporous graphene and the functional applications of vapor phase dealloyed porous Ge as Li ion battery electrodes. This study shines lights on the versatility of vapor phase dealloying for the fabrication of bicontinuous porous materials for a wide range of functional applications.

We used an online aerosol‐climate model (BCC_AGCM2.0_CUACE/Aero) to simulate effective radiative forcing and climate response to changes in the concentrations of short‐lived climatic pollutants (SLCPs), including methane, tropospheric ozone, and black carbon, for the period 2010–2050 under Representative Concentration Pathway scenarios (RCPs) 8.5, 4.5, and 2.6. Under these three scenarios, the global annual mean effective radiative forcing were 0.1, −0.3, and −0.5 W m−2, respectively. Under RCP 8.5, the change in SLCPs caused significant increases in surface air temperature (SAT) in middle and high latitudes of the Northern Hemisphere and significant decreases in precipitation in the Indian Peninsula and equatorial Pacific. Global mean SAT and precipitation increased by 0.13 K and 0.02 mm d−1, respectively. The reduction in SLCPs from 2010 to 2050 under RCPs 4.5 and 2.6 led to significant decreases in SAT at high latitudes in the Northern Hemisphere. Precipitation increased slightly in most continental regions, and the Intertropical Convergence Zone moved southward under both of these mitigation scenarios. Global mean SAT decreased by 0.20 and 0.44 K, and global averaged precipitation decreased by 0.02 and 0.03 mm d−1 under RCPs 4.5 and 2.6, respectively.

Nickel lysine salen complex was successfully synthesized via a stepwise procedure and applied as a heterogeneous catalyst for styrene epoxidation. For comparison, several other transition metal (Mn, Fe, Co, and Cu) lysine salen complexes were also synthesized. The prepared catalysts were characterized by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX). Data obtained by FT-IR and Raman spectroscopy indicated the formation of C=N bonds and the complexation of these bonds with metal ions. SEM analysis revealed that the complexation of metal ions with the C=N involves the change in surface morphology of samples. In addition, atomic percent composition of samples was obtained from EDX spectra, which was the complementary evidence for the formation of complexes. Results of catalytic measurements showed that a high conversion of styrene (91.51%) and selectivity to styrene oxide (91.99%) could be achieved over the nickel lysine salen complex with tert-butyl hydroperoxide as the oxidant. When the catalyst was reused the conversion of styrene decreased but the selectivity to styrene oxide still remained high.

Ammonia borane (AB) has been identified as one of the most promising candidates for chemical hydrogen storage. However, the practical application of AB for hydrogen production is hindered by the need of efficient and inexpensive catalysts. For the first time, we report that the incorporation of Mo into Cu@Co core-shell structure can significantly improve the catalytic efficiency of hydrogen generation from the hydrolysis of AB. The Cu0.81@Mo0.09Co0.10 core-shell catalyst displays high catalytic activity towards the hydrolysis dehydrogenation of AB with a turnover frequency (TOF) value of 49.6 molH2 molcat−1 min−1, which is higher than most of Cu-based catalysts ever reported, and even comparable to those of noble-metal based catalysts. The excellent catalytic performance is attributed to the multi-elements co-deposition effect and electrons transfer effect of Cu, Mo and Co in the tri-metallic core-shell NPs.

Efficient, durable and low-cost electrocatalysts that accelerate sluggish oxygen reduction reaction kinetics are urgently needed for the energy conversion techniques, such as metal-air batteries and fuel cells. In this work, we develop a molten-salt-assisted thermal emitting approach to transform the cheap and easily obtainable bulk ferric (III) oxide powder into a highly efficient Fe single atom catalyst for cathodic oxygen reduction reaction. Benefiting from the strong polarity force of ionized cations and anions, the molten salt effectively facilitates breakage of chemical bonding in bulk Fe2O3 and volatilization of Fe species far below the melting point of Fe2O3 (1841 K), lowering the consumption of energy and time needed in the synthetic procedure. The vaporized Fe species are subsequently anchored onto the surface of nitrogen-doped porous carbon, evolving the single-atom “Fe-N4-O2” site catalyst. The obtained catalyst presents an excellent oxygen reduction reaction performance with half-wave potential of 0.896 V vs RHE in alkaline media, comparable to the most efficient non-precious metal catalysts and outperforming the benchmark system Pt/C. Furthermore, this method is demonstrated to be valid for synthesis of non-noble-metal single atom catalysts (metal = Co, Mn, Cu, Ni) by changing different metal oxides precursors.

We used an online aerosol–climate model to study the equilibrium climate response of the East Asian summer monsoon (EASM) to increases in anthropogenic emissions of sulfate, organic carbon, and black carbon aerosols from 1850 to 2000. Our results show that each of these aerosol species has a different effect on the EASM as a result of changes in the local sea–land thermal contrast and atmospheric circulation. The increased emission of sulfate aerosol leads to a decrease in the thermal contrast between the land and ocean, a southward shift of the East Asian subtropical jet, and significant northerly wind anomalies at 850 hPa over eastern China and the ambient oceans, markedly dampening the EASM. An increase in organic carbon aerosol results in pronounced surface cooling and the formation of an anomalous anticyclone over the oceans north of 30°N. These effects cause a slight increase in the sea–land thermal contrast and southerly flow anomalies to the west of the anticyclonic center, strengthening the northern EASM. An increase in organic carbon emission decreases the sea–land thermal contrast over southern China, which weakens the southern EASM. The response of the summer 850-hPa winds and rainfall over the East Asian monsoon region to an increase in black carbon emission is generally consistent with the response to an increase in organic carbon. The increase in black carbon emission leads to a strengthening of the northern EASM north of 35°N and a slight weakening of the southern EASM south of 35°N. The simulated response of the EASM to the increase in black carbon emission is unchanged when the emission of black carbon is scaled up by five times its year 2000 levels, although the intensities of the response is enhanced. The increase in sulfate emission primarily weakens the EASM, whereas the increases in black carbon and organic carbon emissions mitigate weakening of the northern EASM.

Soot particles strongly absorb sunlight and hence act as a short-lived warming agent. Atmospheric aging of soot particles changes their morphology and mixing state and consequently alters their optical properties. Here we collected soot particles at tunnel, urban, mountaintop, and background sites in the North China Plain and analyzed their mixing structures and morphology using transmission electron microscopy. Soot particles were further classified into three types: bare-like, partly coated, and embedded. Bare-like soot particles were dominant at the tunnel site, while most soot particles were of the partly coated or embedded type at other sites. Fractal dimensions (Df) of different types of soot particles ranged from 1.80 to 2.16 and increased in the following order: bare-like < partly coated < embedded. Moreover, their average Df changed from 1.8 to 2.0 from the tunnel to the background site. We conclude that the Df can characterize the shape of soot aggregates reasonably well, and its variation refle...

Tuning surface structures by bottom-up synthesis has been demonstrated as an effective strategy to improve the catalytic performances of nanoparticle catalysts. Nevertheless, the surface modification of three-dimensional nanoporous metals, fabricated by a top-down dealloying approach, has not been achieved despite great efforts devoted to improving the catalytic performance of three-dimensional nanoporous catalysts. Here we report a surfactant-modified dealloying method to tailor the surface structure of nanoporous gold for amplified electrocatalysis toward methanol oxidation and oxygen reduction reactions. With the assistance of surfactants, {111} or {100} faceted internal surfaces of nanoporous gold can be realized in a controllable manner by optimizing dealloying conditions. The surface modified nanoporous gold exhibits significantly enhanced electrocatalytic activities in comparison with conventional nanoporous gold. This study paves the way to develop high-performance three-dimensional nanoporous catalysts with a tunable surface structure by top-down dealloying for efficient chemical and electrochemical reactions., Tuning the ratio of facets in materials can affect catalytic activity but methods to achieve this can be difficult to control. Here, using surfactants during dealloying, the authors prepare nanoporous gold with controlled facet ratios and show how it can significantly improve electrocatalytic activity.

We examine the roles of fast and slow responses in shaping the total equilibrium response of the East Asian summer monsoon (EASM) to reflecting (sulfate, SO4) and absorbing (black carbon, BC) aerosol forcings over the industrial era using the Community Earth System Model version 1 (CESM1). Our results show that there is a clear distinction between fast and slow responses of the EASM to aerosol forcings and the slow climate response due to aerosol-induced change in sea surface temperature (SST) plays an important role in the impacts of aerosols on the EASM. The EASM is weakened by a decrease in land–sea surface thermal contrast in the fast response (FR) component to SO4 forcing, whereas the weakening is more intensive due to the changes in tropospheric thermodynamic and dynamic structures in the slow response (SR) component to SO4. The total climate adjustment caused by SO4 is a significant weakening of the EASM and a decrease in precipitation. The BC-induced fast adjustment strengthens the EASM both by increasing the local land–sea surface thermal contrast and shifting the East Asian subtropical jet (EASJ) northwards. The BC-induced slow climate adjustment, however, weakens the EASM through altering the atmospheric temperature and circulation. Consequently, the EASM is slightly enhanced, especially north of 30° N, in the total response (TR) to BC. The spatial patterns of precipitation change over East Asia due to BC are similar in the total response and slow response. This study highlights the importance of ocean response to aerosol forcings in driving the changes of the EASM.

Contiguous mudflats exist along the coast zone of the Bohai Bay in the western part of the Bohai Sea of China. They are generally suitable for land reclamation, which can ease the contradiction between land resource shortage and the demand for development, because mudflats are important geomorphological systems which provide land resources. The shoreline at the Bohai Bay has experienced significant changes since 2003 due to high intensity reclamation. Environmental impacts of reclamation at different stages, especially on the tidal wave system, have attracted worldwide attention. However, there is still a lack of understanding on the cumulative response process of the tidal wave system and its response mechanism to high intensity development of mudflats. In this study, six periods were selected as representative reclamation stages in the Bohai Bay: 2003, 2006, 2012, future planning, and 0 m and −5 m reclamation stages. A two-dimensional tidal wave mathematical model was developed to analyze the cumulative changing process and dominant mechanism of the change of the tidal wave system by comparing effects of reclamation of the entire bay and cumulative effects of sequential reclamation projects, as well as studying the relations between the tidal wave change and tidal range on the shore segment where the reclamation project is located. Based on the analysis results, the influence of reclamation in the Bohai Bay on the M2 constituent tide was much greater than that on the K1 constituent tide, especially on the M2 constituent tide amphidromic point outside of the Yellow River mouth. The movement distance of the M2 constituent tide amphidromic point outside the Yellow River mouth for the aforementioned reclamation stages compared to its location in 2003 was about 1, 10, 22.5, 27.3, and 83.8 km, respectively. In contrast, the maximum movement distance of the other M2 constituent tide amphidromic point outside Qinhuangdao and the unique K1 constituent tide amphidromic point in the Bohai Sea for the aforementioned reclamation stages compared to their conditions in 2003 were about 5.9 and 6.3 km, respectively. With the increase of the reclamation scale, the M2 constituent tide amphidromic point outside of the Yellow River mouth first moved southeastward, and then moved northeastward, and the tidal range of the western Bohai Bay first increased and then decreased, while the speed of tidal wave propagation first accelerated and then stabilized during the 0 m reclamation stage. During the −5 m reclamation stage, the tidal wave system experienced obvious changes, however, the tidal properties inside the bay remained the same. The average tidal current velocity of the bay decreased with the increase of the reclamation scale, and the tidal current velocity of 2003, 2012, future planning and −5 m reclamation stages were 0.33–0.37, 0.3–0.34, 0.29–0.31, and 0.16–0.17 m/s, respectively. When reclamation projects were performed one by one counterclockwise from Caofeidian (future planning) in the north to south until all the future planned reclamation projects were completed, the M2 constituent tide amphidromic point first moved southwestward, and then moved southeastward. In semi-closed bays influenced by rotational tidal wave, the impact intensity of reclamation projects to the tidal wave system depends mostly on the tidal range of the coast where the reclamation projects are located. The greater the tidal range, the more obvious the effects of the reclamation projects will be on the tidal wave system. Continuous reclamation will increase flooding risks and tendency of siltation in the bay, weaken the water exchange rate in the bay, affect scouring and silting, as well as impact the design and defense standards of coastal engineering projects near the Yellow River mouth.

China׳s island and reef coasts are mainly distributed along the coasts of Zhejiang and Fujian provinces. These areas have favorable deep-water conditions and great development potential. The Zhoushan Archipelago is a typical island and reef coast. In the midst of the islands, there are many tidal channels with high water depth and high current velocity. Daishan Island is located on the northern part of the Zhoushan Archipelago. The sea north of Daishan Island can be characterized as having great water depth (generally between 10 and 20 m), high current velocity (the maximum measured value is about 2 m/s), and hyperconcentrated suspended sediment (annual average concentration under normal weather conditions is about 0.6 kg/m 3 ). Sediment transport is influenced by the co-action of waves and currents. Land reclamation can ease the contradiction between the desire for development and the land resource shortage on island and reef coasts. Land reclamation generally starts from deposition-promoting projects on island and reef coasts with hyperconcentration of suspended sediment with the aim of reducing the reclamation cost. Based on the morphodynamic characteristics of the sea area near north of Daishan Island, a two-dimensional mathematical model was developed to analyze the co-action of current and waves and the model was verified for spring as well as neap tides in 2007 and 2012. The simulated values of tidal stages, tidal current velocities, tidal current directions, and suspended sediment concentration at 15 stations in 2007 and 9 stations in 2012 were in good agreement with the in-situ measured values. Furthermore, the simulated magnitude and pattern of erosion and sedimentation agreed well with the observations. This model was used to evaluate the effects of a proposed siltation scheme on the hydrodynamic environment and morphological change of the study area. The results of this study can inform the protection, development, and utilization of shoal-channels on island and reef coasts, as well provide a scientific basis for comprehensive maritime protection and development.

The comprehensive effects of anthropogenic aerosols (sulfate, black carbon, and organic carbon) on terrestrial aridity were simulated using an aerosol–climate coupled model system. The results showed that the increase in total anthropogenic aerosols in the atmosphere from 1850 to 2010 had caused global land annual mean precipitation to decrease by about 0.19 (0.18, 0.21) mm day−1, where the uncertainty range of the change (minimum, maximum) is given in parentheses following the mean change, and reference evapotranspiration ET0 (representing evapotranspiration ability) to decrease by about 0.33 (0.31, 0.35) mm day−1. The increase in anthropogenic aerosols in the atmosphere from 1850 to 2010 had caused land annual mean terrestrial aridity to decrease by about 3.0% (2.7%, 3.6%). The areal extent of global total arid and semiarid areas had reduced due to the increase in total anthropogenic aerosols in the atmosphere from preindustrial times. However, it was found that the increase in anthropogenic aerosols in the atmosphere had enhanced the terrestrial aridity and thus resulted in an expansion of arid and semiarid areas over East and South Asia. The projected decrease in anthropogenic aerosols in the atmosphere from 2010 to 2100 will increase global land annual mean precipitation by about 0.15 (0.13, 0.16) mm day−1 and ET0 by about 0.26 (0.25, 0.28) mm day−1, thereby producing a net increase in terrestrial aridity of about 2.8% (2.1%, 3.6%) and an expansion of global total arid and semiarid areas.

Previous modeling work showed that aerosol can affect the position of the tropical rain belt, i.e., the intertropical convergence zone (ITCZ). Yet, it remains unclear which aspects of the aerosol impact are robust across models, and which are not. Here, we present simulations with seven comprehensive atmosphere models that study the fast and slow impacts of an idealized anthropogenic aerosol on the zonal-mean ITCZ position. The fast impact, which results from aerosol atmospheric heating and land cooling before sea-surface temperature (SST) have time to respond, causes a northward ITCZ shift. Yet, the fast impact is compensated locally by decreased evaporation over the ocean, and a clear northward shift is only found for an unrealistically large aerosol forcing. The local compensation implies that while models differ in atmospheric aerosol heating, this does not contribute to model differences in the ITCZ shift. The slow impact includes the aerosol impact on the ocean surface energy balance and is mediated by SST changes. The slow impact is an order of magnitude more effective than the fast impact and causes a clear southward ITCZ shift for realistic aerosol forcing. Models agree well on the slow ITCZ shift when perturbed with the same SST pattern. However, an energetic analysis suggests that the slow ITCZ shifts would be substantially more model dependent in interactive-SST setups due to model differences in clear-sky radiative transfer and clouds. We also discuss implications for the representation of aerosol in climate models and attributions of recent observed ITCZ shifts to aerosol. This article is protected by copyright. All rights reserved.

Recovering pure and viable circulating tumor cells (CTCs) from blood has been a challenging task for molecular characterization and functional analysis, which has attracted wide attention these days. Herein, we fabricate a thermoresponsive chitosan nanofiber substrate to effectively capture, purify, and release the target cancer cells, assisted by PNIPAAm brushes and DNA hybridization. The PNIPAAm brushes are designed to enable WBCs to detach from aptamer-PNIPAAm-chitosan-nanofiber (aptamer-P-CNFs) surfaces during the conformational transition. Meanwhile these specific captured CTCs are retained at a high purity. Moreover, effective and intact release of CTCs from the substrates without any foreign agents is realized by complementary sequences efficiently hybridizing with aptamers, and the specific cell release makes CTCs further purified. The present work provides a new strategy in the design of biointerface for recovering target CTCs from whole blood samples with high purity.

To mitigate the projected global warming in the 21st century, it is well recognized that society needs to cut CO2 emission and other short-lived warming agents aggressively. However, to stabilize the climate at a warming level closer to the present day, such as the well below 2 °C aspiration in the Paris agreement, a net-zero carbon emission by 2050 is still insufficient. The recent IPCC special report calls for a massive scheme to extract CO2 directly from the atmosphere, in addition to the decarbonization, to reach negative net emission at the mid-century mark. Another ambitious proposal is the solar radiation-based geoengineering schemes, including injecting sulfur gas into the stratosphere. Despite being in the public debate for years, these two leading geoengineering schemes have not been carefully examined under a consistent numerical modeling framework. Here we present a comprehensive analysis of climate impacts of these two geoengineering approaches using two recently available large-ensemble (> 10 members) model experiments conducted by a family of state-of-art Earth system models. The CO2-based mitigation simulation is designed to include both emissions cut and carbon capture. The solar radiation-based mitigation simulation is designed to inject the sulfur gas strategically at specified altitudes and latitudes and run a feedback control algorithm, to avoid common problems previously identified such as the over-cooling of the Tropics and large-scale precipitation shifts. Our analysis focuses on the projected aridity conditions over the Americas in the 21st century, in detailed terms of the mitigation potential, the temporal evolution, the spatial distribution (within North and South America), the relative efficiency, and the physical mechanisms. We show that sulfur injection, in contrast to previous notions of leading to excessive terrestrial drying (in terms of precipitation reduction) while offsetting the global mean greenhouse gas (GHG) warming, will instead mitigate the projected drying tendency under RCP8.5. The surface energy balance change induced by Sulfur injection, in addition to the well-known response in temperature and precipitation, plays a crucial role in determining the overall terrestrial hydroclimate response. However, when normalized by the same amount of avoided global warming, in these simulations, sulfur injection is less effective in limiting the worsening trend of regional land aridity in the Americas, when compared with carbon capture. Temporally, the climate benefit of Sulfur injection will emerge more quickly, even when both schemes are hypothetically started in the same year of 2020. Spatially, both schemes are effective in curbing the drying trend over North America. However, for South America, the Sulfur Injection scheme is particularly more effective for the sub-Amazon region (South Brazil), while the Carbon Capture scheme is more effective for the Amazon region. We conclude that despite the apparent limitations (such as inability to address ocean acidification) and potential side effects (such as changes to the ozone layer), innovative means of Sulfur Injection should continue to be explored as a potential low-cost option in the climate solution toolbox, complementing other mitigation approaches such as emissions cut and carbon capture (Cao et al., 2017). Our results demonstrate the urgent need for multi-model comparison studies and detailed regional assessment in other parts of the world.

Extreme rarity and inherent heterogeneity of circulating tumor cells (CTCs) result in a tremendous challenge for the CTC isolation from patient blood samples with high efficiency and purity. Current CTC isolation approaches mainly rely on the epithelial cell adhesion molecule (EpCAM), which may significantly reduce the ability to capture CTCs when the expression of EpCAM is lost or down-regulated in epithelial-mesenchymal transition. Here, a rapid and highly efficient method is developed to isolate and identify heterogeneous CTCs with high efficiency from patient blood samples using the fluorescent-magnetic nanoparticles (F-MNPs). A dual-antibody interface targeting EpCAM and N-cadherin is fabricated onto the F-MNPs to capture epithelial CTCs as well as mesenchymal CTCs from whole blood samples. The poly(carboxybetaine methacrylate) brushes of excellent antifouling properties are employed to decrease nonspecific cell adhesion. Moreover, the F-MNPs provide a prompt identification strategy for heterogeneous CTCs (F-MNPs+, Hoechst 33342+, and CD45-) that can directly identify CTCs in a gentle one-step processing within 1 h after isolation from patient blood samples. This has been demonstrated through artificial samples as well as patient samples in details.

A three-image algorithm is proposed to retrieve the sample's transmission, refraction and dark-field information in hard X-ray grating interferometry. Analytical formulae of the three-image algorithm are theoretically derived and presented, and evaluated by proof-of-principle synchrotron radiation experiments. The results confirm the feasibility of the proposed algorithm. The novelty of the proposed algorithm is that it allows versatile and tunable multimodal X-ray imaging by substantially relaxing the existing limitations on the lateral grating position. Furthermore, this algorithm can also be adapted for samples with negligible refraction, reducing the number of required sample measurements to two. Furthermore, the noise properties of the retrieved images are investigated in terms of the standard deviations. Theoretical models are presented and verified by synchrotron radiation measurements. It is shown that the noise standard deviations exhibit strong dependence on the lateral grating position, especially in the case of refraction and dark-field images. Further noise reduction and dose reduction can thus be possible by optimizing the lateral grating position for a selected region of interest. Those results can serve as general guidelines to optimize the data acquisition scheme for specific applications and problems.

Simple Summary Rhizosphere, where microbes and plants coexist, is a hotspot of mobile genetic element (MGE) transfers. It was suggested that ancient MGE transfers drove the evolution of both microbes and plants. On the other hand, recurrent MGE transfers regulate microbe-plant interaction and the adaptation of microbes and plants to the environment. The studies of MGE transfers in the rhizosphere provide useful information for the research on pathogenic/ beneficial microbe-plant interaction. In addition, MGE transfers between microbes and the influence by plant root exudates on such transfers provide useful information for the research on bioremediation. Abstract The transfer of mobile genetic elements (MGEs) has been known as a strategy adopted by organisms for survival and adaptation to the environment. The rhizosphere, where microbes and plants coexist, is a hotspot of MGE transfers. In this review, we discuss the classic mechanisms as well as novel mechanisms of MGE transfers in the rhizosphere. Both intra-kingdom and cross-kingdom MGE transfers will be addressed. MGE transfers could be ancient events which drove evolution or recurrent events which regulate adaptations. Recent findings on MGE transfers between plant and its interacting microbes suggest gene regulations brought forth by such transfers for symbiosis or defense mechanisms. In the natural environment, factors such as temperature and soil composition constantly influence the interactions among different parties in the rhizosphere. In this review, we will also address the effects of various environmental factors on MGE transfers in the rhizosphere. Besides environmental factors, plant root exudates also play a role in the regulation of MGE transfer among microbes in the rhizosphere. The potential use of microbes and plants for bioremediation will be discussed.

Herein, we fabricated efficient MR imaging probes by incorporating gadolinium oxide nanoparticles (Gd2O3) and gadolinium hybrid nanoparticles (GH) within RBCs. The Gd2O3 and GH encapsulated in the RBCs exhibited high relaxation rates and revealed high sensitivity for T1 MR imaging.

The impact of the total effects due to anthropogenic aerosols from global, East Asian, and non-East Asian sources on East Asian summer monsoon (EASM) system is studied using an aerosol-climate online model BCC_AGCM2.0.1_CUACE/Aero. The results show that the summer mean net all-sky shortwave fluxes averaged over East Asian monsoon region (EAMR) at the top of the atmosphere (TOA) and surface reduce by 4.8 and 5.0 W m − 2 , respectively, due to the increases of global aerosol emissions in 2000 relative to 1850. Changes in radiations and their resulting changes in heat and water transport and cloud fraction contribute together to the surface cooling over EAMR in summer. The increases in global anthropogenic aerosols lead to a decrease of 2.1 K in summer mean surface temperature and an increase of 0.4 hPa in summer mean surface pressure averaged over EAMR, respectively. It is shown that the changes in surface temperature and pressure are significantly larger over land than ocean, thus decreasing the contrast of land-sea surface temperature and pressure. This results in the marked anomalies of north and northeast winds over eastern and southern China and the surrounding oceans in summer, thereby weakening the EASM. The summer mean precipitation averaged over the EAMR reduces by 12%. The changes in non-East Asian aerosol emissions play a more important role in inducing the changes of local temperature and pressure, and thus significantly exacerbate the weakness of the EASM circulation due to local aerosol changes. The weakening of circulation due to both is comparable, and even the effect of non-local aerosols is larger in individual regions. The changes of local and non-local aerosols contribute comparably to the reductions in precipitation over oceans, whereas cause opposite changes over eastern China. Our results highlight the importance of aerosol changes outside East Asia in the impact of the changes of anthropogenic aerosols on EASM.

An atmospheric general circulation model BCC_AGCM2.0 and observation data from ARIS were used to calculate the effective radiative forcing (ERF) due to increased methane concentration since pre-industrial times and its impacts on climate. The ERF of methane from 1750 to 2011 was 0.46 W m−2 by taking it as a well-mixed greenhouse gas, and the inhomogeneity of methane increased its ERF by about 0.02 W m−2. The change of methane concentration since pre-industrial led to an increase of 0.31 °C in global mean surface air temperature and 0.02 mm d−1 in global mean precipitation. The warming was prominent over the middle and high latitudes of the Northern Hemisphere (with a maximum increase exceeding 1.4 °C). The precipitation notably increased (maximum increase of 1.8 mm d−1) over the ocean between 10°N and 20°N and significantly decreased (maximum decrease >–0.6 mm d−1) between 10°S and 10°N. These changes caused a northward movement of precipitation cell in the Intertropical Convergence Zone (ITCZ). Cloud cover significantly increased (by approximately 4%) in the high latitudes in both hemispheres, and sharply decreased (by approximately 3%) in tropical areas.

Greenhouse gases (GHGs) and aerosols are the two most important anthropogenic forcing agents in the 21st century. The expected declines of anthropogenic aerosols in the 21st century from present-day levels would cause an additional warming of the Earth's climate system, which would aggravate the climate extremes caused by GHG warming. We examine the increased rate of precipitation extremes with global mean surface warming in the 21st century caused by anthropogenic GHGs and aerosols, using an Earth system model ensemble simulation. Similar to mean precipitation, the increased rate of precipitation extremes caused by aerosol forcing is significantly larger than that caused by GHG forcing. The aerosol forcing in the coming decades can play a critical role in inducing change in precipitation extremes if a lower GHG emission pathway is adopted. Our results have implications for policy-making on climate adaptation to extreme precipitation events.

Over the last decade, significant progress has been made to develop sensitive devices for the capture of circulating tumor cells (CTCs) from blood of cancer patients. However, simple capture and counting of CTCs cannot provide effective information for understanding the biology of them. In this work, a functional biointerface is fabricated for specific capture and nondestructive release of CTCs from blood samples. A nanostrucure of porous network based on chitosan nanofibers is fabricated by electrospinning, to mimic the function of extracellular matrices, and then the poly(carboxybetaine methacrylate) (pCBMA) brushes integrating onto nanofiber interface provide the effect of interfacial properties to control nonspecific cell adhesion and the multivalent immobilization of aptamers to induce high efficient and specific CTC capture. Furthermore, a complementary sequence is used to efficiently hybridize with the aptamer to achieve nondestructive release of the captured target cells, assisted by the flexible space provided by pCBMA brushes. This work also shows how nanostructure and the interface molecules regulate the morphology of the captured CTCs, and reveals the importance of the controllable cell morphology on biointerface for an effective nondestructive release of the captured CTCs.

This study simulates the effective radiative forcing (ERF) of tropospheric ozone from 1850 to 2013 and its effects on global climate using an aerosol–climate coupled model, BCC AGCM2.0.1 CUACE/Aero, in combination with OMI (Ozone Monitoring Instrument) satellite ozone data. According to the OMI observations, the global annual mean tropospheric column ozone (TCO) was 33.9 DU in 2013, and the largest TCO was distributed in the belts between 30°N and 45°N and at approximately 30°S; the annual mean TCO was higher in the Northern Hemisphere than that in the Southern Hemisphere; and in boreal summer and autumn, the global mean TCO was higher than in winter and spring. The simulated ERF due to the change in tropospheric ozone concentration from 1850 to 2013 was 0.46 W m−2, thereby causing an increase in the global annual mean surface temperature by 0.36°C, and precipitation by 0.02 mm d−1 (the increase of surface temperature had a significance level above 95%). The surface temperature was increased more obviously over the high latitudes in both hemispheres, with the maximum exceeding 1.4°C in Siberia. There were opposite changes in precipitation near the equator, with an increase of 0.5 mm d−1 near the Hawaiian Islands and a decrease of about −0.6 mm d−1 near the middle of the Indian Ocean.