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Anshan-Benxi area in Liaoning province is an important banded iron formations (BIFs) ore-mining district in China. Chlorite is widely distributed in this area, which is related to BIFs and high-grade iron ore, respectively. A fast and convenient method to identify the type and spatial distribution of different chlorites is crucial to the evaluation of high-grade iron ore in this area. Qidashan iron mine is a typical BIFs deposit, and its BIFs-related high-grade iron ore reserves are the second largest in the area. In this paper, the laboratory-based HySpex-320m hyperspectral imaging was used to study the wall rock in Qidashan iron mine. A hyperspectral imaging processing model was established for mineral identification, mineralogy mapping, and chlorite spectral features extraction. The results show that the wavelength positions of OH, Fe-OH, and Mg-OH absorptions of chlorite in the altered wall rock of high-grade iron ore are between 1400 and 1410, 2260 and 2265, and 2360 and 2370 nm, respectively, which are longer than those around BIFs. The relationship between cations in the octahedral layer of chlorite and the wavelengths of OH, Fe-OH, and Mg-OH indicates that Mg and Mg/(Mg + Fe) are inversely related to these wavelengths, whereas Fe is positively related. The wavelengths appear to be weakly influenced by AlVI. Since the bandpass of hyperspectral imaging systems is usually less than 10 nm, these chlorite wavelength differences can be used as a favorable tool for the high-grade iron ore exploration and the iron resources evaluation in the Anshan-Benxi area.