1. High resolution 3D visualization of the spinal cord in a post-mortem murine model
- Author
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Alessia Cedola, Inna Bukreeva, Victor E. Asadchikov, Gabriele Biella, Francesco Brun, Marina Chukalina, Michela Fratini, Alexey Buzmakov, Lorenzo Massimi, Alberto Bravin, Alberto Mittone, N. A. Korolev, Anastasya Ingacheva, Alejandra Sierra, Bukreeva, I., Asadchikov, V., Buzmakov, A., Chukalina, M., Ingacheva, A., Korolev, N. A., Bravin, A., Mittone, A., Biella, G. E. M., Sierra, A., Brun, F., Massimi, L., Fratini, M., Cedola, A., Bukreeva, I, Asadchikov, V, Buzmakov, A, Chukalina, M, Ingacheva, A, Korolev, N, Bravin, A, Mittone, A, Biella, G, Sierra, A, Brun, F, Massimi, L, Fratini, M, and Cedola, A
- Subjects
Phase contrast microscopy ,Central nervous system ,Imaging technique ,FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) ,High resolution ,Imaging techniques ,01 natural sciences ,Article ,law.invention ,010309 optics ,03 medical and health sciences ,Image processing ,Phase contrast ,law ,0103 physical sciences ,medicine ,Spinal canal ,Neuronal organization ,Phase retrieval ,030304 developmental biology ,0303 health sciences ,business.industry ,X ray computed tomography ,X ray imaging ,Spinal cord ,Atomic and Molecular Physics, and Optics ,Visualization ,medicine.anatomical_structure ,Murine model ,spinal cord, mice, phase contrast imaging ,business ,Biotechnology ,Biomedical engineering - Abstract
A crucial issue in the development of therapies to treat pathologies of the central nervous system is represented by the availability of non-invasive methods to study the threedimensional morphology of spinal cord, with a resolution able to characterize its complex vascular and neuronal organization. X-ray phase contrast micro-tomography enables a highquality, 3D visualization of both the vascular and neuronal network simultaneously without the need of contrast agents, destructive sample preparations or sectioning. Until now, high resolution investigations of the post-mortem spinal cord in murine models have mostly been performed in spinal cords removed from the spinal canal. We present here post-mortem phase contrast micro-tomography images reconstructed using advanced computational tools to obtain high-resolution and high-contrast 3D images of the fixed spinal cord without removing the bones and preserving the richness of micro-details available when measuring exposed spinal cords. We believe that it represents a significant step toward the in-vivo application. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
- Published
- 2019