1. Influence of proteolytic enzyme treatment on the changes in volatile compounds and odors of beef longissimus dorsi
- Author
-
Dening Xu, Yue Hu, Hao Li, Shengyong Mao, Taolin Wang, Mingxuan Huang, Liping Wang, Di Zhao, Chunbao Li, and Guanghong Zhou
- Subjects
Bromelain ,Proteases ,Proteolysis ,Muscle Proteins ,01 natural sciences ,Analytical Chemistry ,Hydrolysis ,chemistry.chemical_compound ,0404 agricultural biotechnology ,medicine ,Animals ,Food science ,Amino Acids ,chemistry.chemical_classification ,Volatile Organic Compounds ,biology ,medicine.diagnostic_test ,Chemistry ,010401 analytical chemistry ,Proteolytic enzymes ,food and beverages ,04 agricultural and veterinary sciences ,General Medicine ,Proteinase K ,040401 food science ,0104 chemical sciences ,Amino acid ,Papain ,Red Meat ,Taste ,Odorants ,biology.protein ,Cattle ,Food Science ,Peptide Hydrolases - Abstract
Enzymatic tenderization is extensively applied in the meat industry, whereas its influence on meat flavor has seldom been evaluated. Proteinase K, papain, bromelain and Flavourzyme® were used to treat beef muscle, and the changes in volatile compounds and odors were subsequently analyzed. Proteolysis by proteinase K was found to elevate the average bitterness of the identified peptides by generating peptides with high Q values, whereas proteolysis by papain generated the highest level of amino acids. Enzymatic treatment by Flavourzyme and bromelain significantly elevated the levels of ketones and odors, whereas excessive proteolysis by papain and proteinase K largely reduced the levels of esters and aldehydes. The level of amino acids and degree of hydrolysis were found to be predominant factors that regulated the level of volatiles and odors. These results highlighted the huge influence of enzymatic tenderization on meat flavor, depending on degree of hydrolysis and cleavage pattern of applied proteases.
- Published
- 2020