1. Photoswitchable dynasore analogs to control endocytosis with light
- Author
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Ana Trapero, Pau Gorostiza, Alexandre Gomila-Juaneda, Artur Llobet, Ernest Giralt, Andrés Martín-Quirós, Amadeu Llebaria, Núria Camarero, Ariadna Pérez-Jiménez, Eric Macia, Jordi Hernando, and Laura Nevola
- Subjects
Transient absorption spectroscopies ,Espectroscòpia molecular ,Cell ,Spatiotemporal control ,Pharmacological properties ,010402 general chemistry ,Endocytosis ,01 natural sciences ,Flow cytometry ,03 medical and health sciences ,Photochromism ,Cellular dynamics ,Photo-switchable ,medicine ,030304 developmental biology ,Dynamin ,0303 health sciences ,Interacció cel·lular ,medicine.diagnostic_test ,Single wavelength ,Chemistry ,Micromolar concentration ,Biological activity ,General Chemistry ,Fluorescence ,Molecular spectroscopy ,0104 chemical sciences ,medicine.anatomical_structure ,Cell interaction ,Biophysics ,Small molecule inhibitor - Abstract
Altres ajuts: CERCA Programme/Generalitat de Catalunya Aquest article té una correcció a 10.1039/d0sc90189j The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.
- Published
- 2020