1. The in vivo behavior and antitumor activity of doxorubicin-loaded poly(gamma-benzyl L-glutamate)-block-hyaluronan polymersomes in Ehrlich ascites tumor-bearing BalB/c mice
- Author
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Christophe Schatz, Jean-François Le Meins, Ankur Kaul, Anil K. Mishra, Krishna Chuttani, Ambikanandan Misra, Kamal Kumar Upadhyay, Sébastien Lecommandoux, Pharm Dept, Fac Technol & Eng, Univ Baroda, University of Baroda, India, Div Cyclotron & Radiopharmaceut Sci (DRDO, INMAS), Univ New Delhi, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Team 3 LCPO : Polymer Self-Assembly & Life Sciences, and Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)
- Subjects
Polymersomes ,Biodistribution ,Materials science ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Bioengineering ,Antineoplastic Agents ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,01 natural sciences ,Mice ,Therapeutic index ,In vivo ,medicine ,polycyclic compounds ,Doubling time ,Animals ,Humans ,Ehrlich ascites tumor (EAT) ,General Materials Science ,Doxorubicin ,Hyaluronic Acid ,Carcinoma, Ehrlich Tumor ,Hyaluronan ,Drug Carriers ,Mice, Inbred BALB C ,Technetium ,Receptor-mediated endocytosis ,Neoplasms, Experimental ,021001 nanoscience & nanotechnology ,Endocytosis ,0104 chemical sciences ,3. Good health ,Hyaluronan Receptors ,[CHIM.POLY]Chemical Sciences/Polymers ,Polyglutamic Acid ,Immunology ,Polymersome ,Molecular Medicine ,Nanoparticles ,Female ,Rabbits ,0210 nano-technology ,Drug carrier ,Antitumor activity ,medicine.drug - Abstract
The in vivo efficacy of doxorubicin (DOX)-loaded poly(γ-benzyl l-glutamate)- block -hyaluronan (PBLG 23 - b -HYA 10 )-based polymersomes (PolyDOX) was evaluated. Samples were efficiently labeled with technetium-99m radionuclide with good stability for in vivo studies. PolyDOX enhanced circulation time compared to free DOX. Biodistribution studies revealed selective accumulation of PolyDOX in the Ehrlich ascites tumor (EAT) as a result of passive accumulation and active targeting (CD44-mediated endocytosis) in EAT-bearing mice. Toxicity studies demonstrated PolyDOX is a safe drug carrier, and no hemolysis was observed with PolyDOX equivalent to 200 μg/mL of free DOX. PolyDOX dominantly controlled tumor growth by delaying doubling time of EATs compared to free DOX over 30 days after treatment. PolyDOX also increased life span six times more than free DOX. Hence, it is reasonable to expect that higher DOX levels attributable to PolyDOX improve the therapeutic index and reduce side effects due to site-specific drug accumulation. From the Clinical Editor In this preclinical project, doxorubicin loaded polymersomes enhanced intracellular uptake of doxorubicin in a murine model of Ehrlich Ascites Tumor (EAT) through CD44 receptor mediated endocytosis, resulting in prolonged Tumor Doubling Time and increase in life span of mice.
- Published
- 2012