1. Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats
- Author
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Mitsuru Tanaka, Xiaojing Sheng, Yusuke Amiya, Kenichiro Tsukamoto, Toshiro Matsui, Masato Beppu, Satoshi Nagaoka, Marika Hashimoto, and Emiko Yanase
- Subjects
Male ,Maldi ms ,Administration, Oral ,Pharmacology ,Mass spectrometry ,01 natural sciences ,Analytical Chemistry ,0404 agricultural biotechnology ,Oral administration ,Animals ,Rats, Wistar ,Oligopeptide ,Plasma samples ,Chemistry ,Seed Storage Proteins ,010401 analytical chemistry ,Globulins ,Tail vein ,04 agricultural and veterinary sciences ,General Medicine ,Antigens, Plant ,040401 food science ,Peptide Fragments ,0104 chemical sciences ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Plasma concentration ,Soybean Proteins ,Peptides ,Chromatography, Liquid ,Food Science ,β conglycinin - Abstract
In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after administration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography-time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75–756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats.
- Published
- 2021