1. Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support
- Author
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Tamara Renata Machado Ribeiro, Pedro P. Corbi, Ana Lúcia Tasca Gois Ruiz, Tuany Zambroti Cândido, Amanda Maria Claro, Bruna Cardinali Lustri, Amanda de Lima Pizi Cândido, Fernando Rogério Pavan, Nayara Ap. Simei Aquaroni, Silmara C. Lazarini, Cristiano G. Moreira, Camila Maríngolo Ribeiro, Flávia Aparecida Resende, Douglas Hideki Nakahata, João Carvalho, Wilton R. Lustri, Carmen Silvia Passos Lima, Hernane da Silva Barud, University of Araraquara – UNIARA, Universidade Estadual de Campinas (UNICAMP), and Universidade Estadual Paulista (Unesp) more...
- Subjects
Silver ,Mutagenic activity ,Microbial Sensitivity Tests ,Antiproliferative activity ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Ames test ,4-Aminobenzoic acid ,Inorganic Chemistry ,chemistry.chemical_compound ,Minimum inhibitory concentration ,Silver complex ,In vivo ,Drug release system ,Cellulose ,Cell Proliferation ,Bacteria ,010405 organic chemistry ,Biofilm ,In vitro ,Anti-Bacterial Agents ,0104 chemical sciences ,DNA interaction ,chemistry ,Bacterial cellulose ,Delayed-Action Preparations ,Antibacterial activity ,4-Aminobenzoic Acid - Abstract
Made available in DSpace on 2020-12-12T02:49:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula [Ag (C7H7NO2)2(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The antiproliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer. University of Araraquara – UNIARA Institute of Chemistry University of Campinas – UNICAMP Faculty of Pharmaceutical Sciences University of Campinas – UNICAMP São Paulo State University – UNESP School of Pharmaceutical Sciences Faculty of Medical Sciences University of Campinas – UNICAMP São Paulo State University – UNESP School of Pharmaceutical Sciences FAPESP: , 2015/09833-0 CAPES: 001 FAPESP: 019/03049-7 FAPESP: 13/07276-1 FAPESP: 2014/06779-2 FAPESP: 2015/20882-3 FAPESP: 2016/07729-4 FAPESP: 2017/16278-9 FAPESP: 2017/19243-1 FAPESP: 2018/00163-0 FAPESP: 2018/02344-2 FAPESP: 2018/12062-4 FAPESP: 2018/12590-0 FAPESP: 2018/25512-8 CNPq: 300968/2016-7 CNPq: 407822/2018 more...
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- 2020