1. Translocation of an Intracellular Protein via Peptide-Directed Ligation
- Author
-
Nicolas Brauckhoff, Hazem Salamon, Marcel Schmidt, Tom N. Grossmann, Petra Janning, Christiane Stiller, Dennis M. Krüger, Organic Chemistry, and AIMMS
- Subjects
Peptide ,Chromosomal translocation ,Molecular Dynamics Simulation ,010402 general chemistry ,Protein labeling ,Ligands ,01 natural sciences ,Biochemistry ,Molecular dynamics ,Journal Article ,Humans ,Peptide ligand ,chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,Intracellular protein ,Proteins ,General Medicine ,0104 chemical sciences ,Cell biology ,Transport protein ,Protein Transport ,Molecular Medicine ,Ligation ,Peptides ,HeLa Cells - Abstract
Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number of appropriate ligands and the lack of design principles. Addressing these limitations, we report a proximity-induced labeling system which applies a moderate affinity peptide ligand. The design process was structure-guided and supported by molecular dynamics simulations. We show that selective protein labeling can be performed inside living cells enabling the subcellular translocation of a protein via ligand-directed chemistry for the first time.
- Published
- 2017
- Full Text
- View/download PDF