1. Intrahepatic cholestasis induced by α-naphthylisothiocyanate can cause gut-liver axis disorders
- Author
-
Yuhuan Wu, Zhu Dun, Chuanlan Sang, Qing Wang, Jiamin Fang, Changlian Gan, Tajie Luosang, Zhengyuan Chen, Tian-Qin Xiong, Dawa Zeren, and Bixia Wang
- Subjects
Liver Cirrhosis ,Male ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,α naphthylisothiocyanate ,Cholestasis, Intrahepatic ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Gastroenterology ,Bile Acids and Salts ,03 medical and health sciences ,Subcutaneous injection ,Feces ,food ,Cholestasis ,Internal medicine ,Intestine, Small ,medicine ,Animals ,030304 developmental biology ,0105 earth and related environmental sciences ,Pharmacology ,0303 health sciences ,business.industry ,Transporter ,Small intestine inflammation ,General Medicine ,Single injection ,Hyperplasia ,medicine.disease ,food.food ,Mice, Inbred C57BL ,Intestinal Diseases ,1-Naphthylisothiocyanate ,Liver ,Cytokines ,Ademetionine ,business - Abstract
Clinical studies have shown that Intrahepatic cholestasis is closely related to intestinal injury. The gut-liver axis theory suggests that the intestine and liver are closely related, and that bile acids are important mediators linking the intestine and liver. We compared two cholestasis models: a single injection model that received a single subcutaneous ANIT injection (75 mg/kg), and a multiple subcutaneous injection model that received an injection of ANIT (50 mg/kg) every other day for 2 weeks. We used Transmetil (ademetionine 1,4-butanedisulfonate) to relieve intrahepatic cholestasis in the multiple injection group. In the multiple injection group, we found increased hepatic bile duct hyperplasia, increased fibrosis of the liver, increased small intestine inflammation and oxidative damage, increased harmful bile acids, decreased bile acids transporter levels. After treatment with Transmetil, the liver and gut injuries were relieved. These results suggest that intrahepatic cholestasis can cause disorders of the gut-liver axis.
- Published
- 2020