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Start Over Author "Anne-Sophie Lepeuple" Topic 010501 environmental sciences
6 results on '"Anne-Sophie Lepeuple"'

1. Assessing the bias linked to DNA recovery from biofiltration woodchips for microbial community investigation by fingerprinting

Author

Franck Poly, Léa Cabrol, Jean-Louis Fanlo, Luc Malhautier, Anne-Sophie Lepeuple, Veolia Environnement, Water Research Center, Veolia Environnement, Laboratoire de Génie de l'Environnement Industriel (LGEI), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Laboratoire de Génie de l'Environnement Industriel ( LGEI ), IMT - Mines Alès Ecole Mines - Télécom ( IMT - MINES ALES ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
Sonication, DNA extraction . Cell dispersion . DGGE . Biofiltration . Diversity . Microbial community structure, 010501 environmental sciences, Biology, Nucleic Acid Denaturation, Polymerase Chain Reaction, 01 natural sciences, Applied Microbiology and Biotechnology, [ SDV.EE ] Life Sciences [q-bio]/Ecology, environment, law.invention, 03 medical and health sciences, chemistry.chemical_compound, law, Cluster Analysis, Polymerase chain reaction, 0105 earth and related environmental sciences, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, 0303 health sciences, Chromatography, 030306 microbiology, Extraction (chemistry), Biodiversity, DNA, General Medicine, DNA Fingerprinting, Wood, DNA extraction, chemistry, Nucleic acid, Electrophoresis, Polyacrylamide Gel, Woodchips, Metagenomics, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Filtration, Temperature gradient gel electrophoresis, Biotechnology
Abstract

International audience; In this study, we explored methodological aspects of nucleic acid recovery from microbial communities involved in a gas biofilter filled with pine bark woodchips. DNA was recovered indirectly in two steps, comparing different methods: cell dispersion (crushing, shaking, and sonication) and DNA extraction (three commercial kits and a laboratory protocol). The objectives were (a) to optimize cell desorption from the packing material and (b) to compare the 12 combinations of desorption and extraction methods, according to three relevant criteria: DNAyield, DNA purity, and community structure representation by denaturing gradient gel electrophoresis (DGGE). Cell dispersion was not influenced by the operational parameters tested for shaking and blending, while it increased with time for sonication. DNA extraction by the laboratory protocol provided the highest DNA yields, whereas the best DNA purity was obtained by a commercial kit designed for DNA extraction from soil. After successful PCR amplification, the 12 methods did not generate the same bias in microbial community representation. Eight combinations led to high diversity estimation, independently of the experimental procedure. Among them, six provided highly similar DGGE profiles. Two protocols generated a significantly dissimilar community profile, with less diversity. This study highlighted the crucial importance of DNA recovery bias evaluation.

Published
2009
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2. Understanding of polyhydroxybutyrate production under carbon and phosphorus-limited growth conditions in non-axenic continuous culture

Author

Anne-Sophie Lepeuple, Jean-Louis Uribelarrea, Guillermina Hernandez-Raquet, Maria G.E. Albuquerque, Laëtitia Cavaillé, Estelle Grousseau, Etienne Paul, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche sur l’Eau, Veolia Environnement, Veolia Environment Research and Innovation, French National Research and Technology Association (ANRT, Cifre), ANR-08-ECOT-0017,VALORIA,Développement d'une nouvelle filière de traitement et de valorisation des sous-produits organiques issus de l'assainissement(2008), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), French National Research Agency (VALORIA project) [ANR-08-ECOT-O17-001], and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Mixed population, Environmental Engineering, PHB production, Polyesters, Microorganism, [SDV]Life Sciences [q-bio], Microbial Consortia, Population, chemistry.chemical_element, Bioengineering, macromolecular substances, 010501 environmental sciences, 01 natural sciences, Polyhydroxybutyrate, Open continuous culture, 03 medical and health sciences, education, Axenic, Waste Management and Disposal, 0105 earth and related environmental sciences, education.field_of_study, Bacteria, Sewage, biology, Renewable Energy, Sustainability and the Environment, Polyhydroxyalkanoates, Phosphorus, Continuous reactor, technology, industry, and agriculture, General Medicine, biology.organism_classification, Pulp and paper industry, Carbon, 6. Clean water, Dilution, 030104 developmental biology, Activated sludge, chemistry, Chemical engineering, lipids (amino acids, peptides, and proteins)
Abstract

In a waste into resource strategy, a selection of polyhydroxybutyrate (PHB)-accumulating organisms from activated sludge was achieved in an open continuous culture under acetic acid and phosphorus limitation. Once the microbial population was selected at a dilution rate (D), an increase in phosphorus limitation degree was applied in order to study the intracellular phosphorus plasticity of selected bacteria and the resulting capacity to produce PHB. Whatever D, all selected populations were able to produce PHB. At a D, the phosphorus availability determined the phosphorus-cell content which in turn fixed the amount of cell. All the remaining carbon was thus directed toward PHB. By decreasing D, microorganisms adapted more easily to higher phosphorus limitation leading to higher PHB content. A one-stage continuous reactor operated at D = 0.023 h (1) gave reliable high PHB productivity with PHB content up to 80%. A two-stage reactor could ensure better productivity while allowing tuning product quality.

Published
2016
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3. Polyhydroxybutyrate production by direct use of waste activated sludge in phosphorus-limited fed-batch culture

Author

Etienne Paul, Jean-Louis Uribelarrea, Guillermina Hernandez-Raquet, Anne-Sophie Lepeuple, Laëtitia Cavaillé, Estelle Grousseau, Mathieu Pocquet, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche sur l’Eau, Veolia Environnement, French National Research Agency (VALORIA project), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Environmental Engineering, Time Factors, [SDV]Life Sciences [q-bio], Polyesters, chemistry.chemical_element, Bioengineering, macromolecular substances, 010501 environmental sciences, 01 natural sciences, Acclimatization, Polyhydroxybutyrate, Direct accumulation, Acetic acid, chemistry.chemical_compound, 010608 biotechnology, RNA, Ribosomal, 16S, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Food science, Waste activated sludge, Polyhydroxybutyrate (PHB), Waste Management and Disposal, 0105 earth and related environmental sciences, 2. Zero hunger, Biological Oxygen Demand Analysis, biology, Bacteria, Sewage, Renewable Energy, Sustainability and the Environment, business.industry, Phosphorus, technology, industry, and agriculture, General Medicine, biology.organism_classification, Biotechnology, Fed-batch culture, Butyrates, Activated sludge, chemistry, Microbial population biology, P limitation, Batch Cell Culture Techniques, Feasibility Studies, lipids (amino acids, peptides, and proteins), business
Abstract

International audience; Polyhydroxybutyrate (PHB) production directly by waste activated sludge (WAS) was investigated in aerobic fed-batch conditions using acetic acid as substrate. PHB production was induced by phosphorus limitation. WAS of different origin were tested with various degrees of phosphorus limitation and PHB contents of up to 70% (gCOD(PHB)/gCOD(particulate)) were obtained. This strategy showed the importance of maintaining cell growth for PHB production in order to increase PHB concentration and that the degree of phosphorus limitation has a direct impact on the quantity of PHB produced. Pyrosequencing of 165 rRNA transcripts showed changes in the active bacteria of the WAS microbial community as well as the acclimation of populations depending on sludge origin. The monitoring of the process appeared as the key factor for optimal PHB production by WAS. Different strategies are discussed and compared in terms of carbon yield and PHB content with the feast and famine selection process.

Published
2013
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4. Resistance and resilience of removal efficiency and bacterial community structure of gas biofilters exposed to repeated shock loads

Author

Jean-Louis Fanlo, Léa Cabrol, Luc Malhautier, Anne-Sophie Lepeuple, Xavier Le Roux, Franck Poly, Laboratoire de Génie de l'Environnement Industriel (LGEI), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Veolia Environnement, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Veolia Environnement Recherche et Innovation (VERI), Maisons-Laffitte, France [CIFRE 2006/497], Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Laboratoire de Génie de l'Environnement Industriel ( LGEI ), IMT - Mines Alès Ecole Mines - Télécom ( IMT - MINES ALES ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS )

Subjects
DNA, Bacterial, Time Factors, Environmental Engineering, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Biofiltration, [SDE.MCG]Environmental Sciences/Global Changes, Resistance, Bioengineering, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 010501 environmental sciences, Biology, 01 natural sciences, [ SDE.IE ] Environmental Sciences/Environmental Engineering, 03 medical and health sciences, Bioreactors, Ammonia, RNA, Ribosomal, 16S, Biomass, DGGE, Waste Management and Disposal, Phylogeny, 0105 earth and related environmental sciences, Volatile Organic Compounds, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, 0303 health sciences, Bacteria, Resilience, Denaturing Gradient Gel Electrophoresis, 030306 microbiology, Renewable Energy, Sustainability and the Environment, [SDE.IE]Environmental Sciences/Environmental Engineering, Environmental engineering, Community structure, [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology, Biodiversity, General Medicine, Biodegradation, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [ SDE.MCG ] Environmental Sciences/Global Changes, Biodegradation, Environmental, Environmental chemistry, Biofilter, Gases, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Shock intensity, Filtration, Temperature gradient gel electrophoresis
Abstract

International audience; h i g h l i g h t s " Four biofilters were exposed to repeated substrate pulses of increasing intensity. " New quantitative indices of resistance and resilience capacity were proposed. " We highlighted different robustness levels according to the contaminants. " There was an impact of the perturbation regime at the community level (DGGE). " Bacterial community structure partially coupled to removal efficiency robustness. a b s t r a c t Since full-scale biofilters are often operated under fluctuating conditions, it is critical to understand their response to transient states. Four pilot-scale biofilters treating a composting gas mixture and undergoing repeated substrate pulses of increasing intensity were studied. A systematic approach was proposed to quantify the resistance and resilience capacity of their removal efficiency, which enabled to distinguish between recalcitrant (ammonia, DMDS, ketones) and easily degradable (esters and aldehyde) compounds. The threshold of disturbing shock intensity and the influence of disturbance history depended on the contaminant considered. The spatial and temporal distribution of the bacterial community structure in response to the perturbation regime was analysed by Denaturing Gradient Gel Electrophoresis (DGGE). Even if the substrate-pulses acted as a driving force for some community characteristics (community stratification), the structure-function relationships were trickier to evidence: the distributions of resistance and composition were only partially coupled, with contradictory results depending on the contaminant considered.

Published
2012
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5. Bacterial dynamics in steady-state biofilters: beyond functional stability

Author

Jean-Louis Fanlo, Franck Poly, Luc Malhautier, Anne-Sophie Lepeuple, Léa Cabrol, Laboratoire de Génie de l'Environnement Industriel (LGEI), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Veolia Environnement, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Veolia Environnement Recherche et Innovation [2006/497], Laboratoire de Génie de l'Environnement Industriel ( LGEI ), IMT - Mines Alès Ecole Mines - Télécom ( IMT - MINES ALES ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, biofiltration, [SDE.MCG]Environmental Sciences/Global Changes, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 010501 environmental sciences, Biology, 01 natural sciences, Applied Microbiology and Biotechnology, Microbiology, Acclimatization, [ SDE.IE ] Environmental Sciences/Environmental Engineering, Persistence (computer science), 03 medical and health sciences, chemistry.chemical_compound, stratification, Ammonia, Dimethyl disulfide, 030304 developmental biology, 0105 earth and related environmental sciences, Air Pollutants, Volatile Organic Compounds, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, 0303 health sciences, Bacteria, Ecology, [SDE.IE]Environmental Sciences/Environmental Engineering, Community structure, Reproducibility of Results, [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology, Biodiversity, dynamics, Contamination, stability, Adaptation, Physiological, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Refuse Disposal, acclimatization, [ SDE.MCG ] Environmental Sciences/Global Changes, Biodegradation, Environmental, Microbial population biology, chemistry, Environmental chemistry, Biofilter, microbial community, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Filtration, Temperature gradient gel electrophoresis
Abstract

International audience; The spatial and temporal dynamics of microbial community structure and function were surveyed in duplicated woodchip-biofilters operated under constant conditions for 231 days. The contaminated gaseous stream for treatment was representative of composting emissions, included ammonia, dimethyl disulfide and a mixture of five oxygenated volatile organic compounds. The community structure and diversity were investigated by denaturing gradient gel electrophoresis on 16S rRNA gene fragments. During the first 42 days, microbial acclimatization revealed the influence of operating conditions and contaminant loading on the biofiltration community structure and diversity, as well as the limited impact of inoculum compared to the greater persistence of the endogenous woodchip community. During long-term operation, a high and stable removal efficiency was maintained despite a highly dynamic microbial community, suggesting the probable functional redundancy of the community. Most of the contaminant removal occurred in the first compartment, near the gas inlet, where the microbial diversity was the highest. The stratification of the microbial structures along the filter bed was statistically correlated to the longitudinal distribution of environmental conditions (selective pressure imposed by contaminant concentrations) and function (contaminant elimination capacity), highlighting the central role of the bacterial community. The reproducibility of microbial succession in replicates suggests that the community changes were presumably driven by a deterministic process.

Published
2012
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6. Shock loading in biofilters: impact on biodegradation activity distribution and resilience capacity

Author

Léa Cabrol, Jean-Louis Fanlo, Anne-Sophie Lepeuple, Luc Malhautier, Franck Poly, Veolia Environnement, Water Research Center, Veolia Environnement, Laboratoire de Génie de l'Environnement Industriel (LGEI), IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Laboratoire de Génie de l'Environnement Industriel ( LGEI ), IMT - Mines Alès Ecole Mines - Télécom ( IMT - MINES ALES ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS )

Subjects
Environmental Engineering, biofiltration, chemistry.chemical_element, Biomass, 02 engineering and technology, 010501 environmental sciences, microbial ecology, 01 natural sciences, complex mixtures, Waste Disposal, Fluid, [ SDV.EE ] Life Sciences [q-bio]/Ecology, environment, Bioreactors, perturbations, 0105 earth and related environmental sciences, Water Science and Technology, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, Volatile Organic Compounds, Sewage, Environmental engineering, Contamination, Biodegradation, 021001 nanoscience & nanotechnology, Pulp and paper industry, Sulfur, 6. Clean water, Waste treatment, Biodegradation, Environmental, chemistry, Microbial population biology, 13. Climate action, Biofilter, composting, Environmental science, Woodchips, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, 0210 nano-technology
Abstract

International audience; A synthetic contaminated gas was generated, representative of gaseous emissions from sludge composting. It was composed of six volatile organic compounds (aldehyde, ketones, esters, sulphur compound) in an ammoniacal matrix. The gaseous stream was purified by biofiltration, in pilot scale biofilters filled with pine bark woodchips as organic carrier for biomass colonization. After reaching a constant high efficiency, with complete removal, the system was disturbed by transient loading shocks. The impact of perturbations was assessed by both performance evaluation (i.e. contaminant removal) and microbial behaviour. The microbial community was analysed in terms of density. The resilience of functional component following a perturbation was evaluated. This work highlighted the longitudinal distribution of both biodegradation activities and biomass density.

Published
2009
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