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Background. The urban area continues growing, and in most cases, the planning of green areas does not include in the species selection their physiological characteristics, which determine plant survival. Question/Hypothesis. Stomatal conductance (gS) is a key variable that provides information on how the environment affects a plant physiology. Therefore it is important to inquire how gS is affected in an urban site. Studied species/Data description. Data of environmental variables (photosynthetically active radiation, PAR; air temperature, T A; vapor pressure deficit, VPD ), leaf water potential, ?; and gS were collected in four species Ficus retusa , Fraxinus uhdei, Ligustrum lucidum and Quercus rugosa . Study site and years of study. Two sites, in the center and in the rural area, were selected in Puebla, Mexico, during the wet and dry seasons (2012-2013). Methods. Environmental variables, gS and ? were measured with a diffusion porometer and a pressure bomb through the day every two hours in apparently healthy leaves. Also an experiment under greenhouse conditions was performed. Results. Maximum values of PAR, T A and VPD were registered in the city center in the dry season, whereas maximum gS and ? were found in the rural area in the wet season. ? and gS were significantly higher in F. retusa followed by F. uhdei, whereas the lower values were observed in L. lucidum and Q. rugosa . All variables affected gS for all species. Conclusions. The results demonstrate that it is possible to recommend Ficus retusa and Fraxinus uhdei as suitable species to ameliorate the urban environment.