1. Effect of some pocess parameters in enzymatic dyeing of wool
- Author
-
Andrea Zille, Tzanko Tzanov, Carla J. S. M. Silva, Jovita Oliveira, Artur Cavaco-Paulo, and Universidade do Minho
- Subjects
0106 biological sciences ,Time Factors ,Catechols ,Dye precursors ,Bioengineering ,Resorcinol ,Phenylenediamines ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,Animals ,Full factorial design ,Modifiers full factorial design ,Coloring Agents ,Molecular Biology ,030304 developmental biology ,Laccase ,Analysis of Variance ,0303 health sciences ,Catechol ,Chromatography ,Enzymatic dyeing ,Science & Technology ,biology ,Chemistry ,Wool ,Modifiers ,Temperature ,Resorcinols ,General Medicine ,Factorial experiment ,Hydrogen-Ion Concentration ,Enzyme assay ,Scientific method ,biology.protein ,Dyeing ,Biotechnology - Abstract
This article reports on the dyeing of wool using an enzymatic system comprising laccase; dye precursor, 2,5-diaminobenzenesulfonic acid; and dye modifiers, catechol and resorcinol. Enzymatic dyeing was performed as a batchwise process at the temperature and pH of maximum enzyme activity. The effects of the process variables reaction time, enzyme, and modifier concentration on fabric color were studied, according to an appropriate experimental design. Different hues and depths of shades could be achieved by varying the concentration of the modifiers and the time of laccase treatment. The duration of the enzymatic reaction appeared to be the most important factor in the dyeing process. Thus, the dyeing process, performed at low temperature and mild pH, was advantageous in terms of reduced enzyme and chemical dosage.
- Published
- 2003