1. The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles
- Author
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Di Rong, Daming Wu, Diya Leng, Ruizhen Liang, Yang Zhou, Yan Li, Cuifeng Zhang, Wang Ying, Jie Zhu, Jin Li, and Mingming Li
- Subjects
Biophysics ,Pharmaceutical Science ,Nanoparticle ,chemistry.chemical_element ,Bioengineering ,02 engineering and technology ,Zinc ,010402 general chemistry ,01 natural sciences ,Enterococcus faecalis ,Biomaterials ,chemistry.chemical_compound ,Drug Discovery ,Cytotoxicity ,biology ,Organic Chemistry ,Biofilm ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Calcium silicate ,0210 nano-technology ,Mesoporous material ,Antibacterial activity ,Nuclear chemistry - Abstract
Background Mesoporous calcium-silicate nanoparticles (MCSNs) have good prospects in the medical field due to their great physicochemical characteristics, antibacterial activity and drug delivery capacity. This study was to analyze the antibiofilm activity and mechanisms of silver (Ag) and zinc (Zn) incorporated MCSNs (Ag/Zn-MCSNs) with different percentages of Ag and Zn. Methods Ag/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by Enterococcus faecalis (E. faecalis) treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by E. faecalis biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with E. faecalis. Then, SEM and CLSM were used to observe the survival of E. faecalis on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits. Results The Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against E. faecalis biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation. Conclusion Ag/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.
- Published
- 2020
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