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The insight of biological microstructures is at the basis of understanding the mechanical features and the potential pathologies of tissues, like the blood vessels. Different techniques are available for this purpose, like the Small Angle Light Scattering (SALS) approach. The SALS method has the advantage of being fast and non-destructive, however investigation of its physical principles is still required. Within this work, a numerical study for SALS irradiation of soft biological fibrous tissues was carried out through in-silico simulations based on a Monte Carlo approach to evaluate the effect of the thickness of the specimen. Additionally, the numerical results were validated with an optical setup based on SALS technique for the characterization of fibrous samples with dedicated tests on four 3D-printed specimens with different fibers architectures. The simulations revealed two main regions of interest according to the thickness (thk) of the analyzed media: a Fraunhofer region (thk &lt, 0.6 mm) and a Multiple Scattering region (thk &gt, 1 mm). Semi-quantitative information about the tissue anisotropy was successfully gathered by analyzing the scattered light spot. Moreover, the numerical results revealed a remarkable coherence with the experimental data, both in terms of mean orientation and dispersion of fibers.

Insights into the mechanical and microstructural status of biological soft tissues are fundamental in analyzing diseases. Biaxial traction is the gold standard approach for mechanical characterization. The state of the art methods for microstructural assessment have different advantages and drawbacks. Small angle light scattering (SALS) represents a valuable low energy technique for soft tissue assessment. The objective of the current work was to develop a bench test integrating mechanical and microstructural characterization capabilities for tissue specimens. The setup’s principle is based on the integration of biaxial traction and SALS analysis. A dedicated control application was developed with the objective of managing the test procedure. The different components of the setup are described and discussed, both in terms of hardware and software. The realization of the system and the corresponding performances are then presented.