1. Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
- Author
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Jihun Kang, Young-Shik Yun, Jong-Souk Yeo, In Sik Yun, Seungmuk Ji, and Eun-Hye Kang
- Subjects
0303 health sciences ,Materials science ,Morphology (linguistics) ,lcsh:QH201-278.5 ,Scanning electron microscope ,High resolution ,02 engineering and technology ,General Medicine ,021001 nanoscience & nanotechnology ,Biocompatible material ,Soft lithography ,Polyurethane acrylate ,03 medical and health sciences ,Cell behavior ,Human adipose-derived stem cell ,0210 nano-technology ,lcsh:Microscopy ,030304 developmental biology ,Biomedical engineering ,Nanopillar ,Novus Imago - Abstract
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections. more...
- Published
- 2020