1. Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus
- Author
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Eva Ramon, Tzanko Tzanov, Javier Hoyo, Susana Sanchez-Gomez, Kristina Ivanova, Aleksandra Asenova Ivanova, Universitat Politècnica de Catalunya. Departament d'Enginyeria Química, Universitat Politècnica de Catalunya. Doctorat en Polímers i Biopolímers, and Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial more...
- Subjects
antibiotic resistance ,Antibiotic resistance ,02 engineering and technology ,medicine.disease_cause ,Cell morphology ,Essential oil ,zein nanocapsules ,Anti-Infective Agents ,selective antibacterial activity ,General Materials Science ,Molecular Targeted Therapy ,0303 health sciences ,biology ,Drug Resistance, Microbial ,Staphylococcal Infections ,021001 nanoscience & nanotechnology ,Antimicrobial ,3. Good health ,Selective antibacterial activity ,Estafilococs daurats ,Staphylococcus aureus ,Pseudomonas aeruginosa ,0210 nano-technology ,Research Article ,Materials science ,Targeting antibody ,Antibiòtics ,Microbial Sensitivity Tests ,Staphylococcal infections ,behavioral disciplines and activities ,Antibodies ,Nanocapsules ,essential oil ,Microbiology ,03 medical and health sciences ,Enginyeria química [Àrees temàtiques de la UPC] ,mental disorders ,Oils, Volatile ,medicine ,Humans ,Staphylococcal Protein A ,030304 developmental biology ,Malalties bacterianes ,Fibroblasts ,biology.organism_classification ,medicine.disease ,targeting antibody ,Zein nanocapsules ,Bacteria - Abstract
Targeted bactericide nanosystems hold significant promise to improve the efficacy of existing antimicrobials for treatment of severe bacterial infections, minimizing the side effects and lowering the risk of the development of antibiotic resistance. In this work, we developed antibody-functionalized nanocapsules (NCs) containing antibacterial essential oil (EO) for selective and effective eradication of Staphylococcus aureus. Antibacterial EO NCs were produced via self-assembly nanoencapsulation in the plant-derived protein zein. The obtained EO NCs were decorated with aminocellulose to provide more reactive surface groups for carboxyl-to-amine immobilization of a antibody that is specific against S. aureus. The antibody-enabled EO NCs (Ab@EO NCs) demonstrated 2-fold higher bactericidal efficacy against the targeted bacterium compared to the pristine EO NCs at the same concentrations. The improved antibacterial effect of the Ab@EO NCs toward S. aureus was also confirmed in a real-time assay by monitoring bacterial cells elimination using a quartz crystal microbalance. Furthermore, the Ab@EO NCs selectively decreased the load and changed the cell morphology of the targeted S. aureus in a mixed inoculum with nontargeted Pseudomonas aeruginosa. Applying the nanoformulated antibacterial actives to an in vitro coculture model of the bacteria and skin fibroblasts resulted in suppression of S. aureus growth while preserving the human cells viability. The novel antibody-enabled antibacterial NCs showed potential for improving the treatment efficacy of staphylococcal infections, minimally affecting the beneficial microbial and human cells. more...
- Published
- 2020