1. An accurate, high-speed, portable bifunctional electrical detector for COVID-19
- Author
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Fang Xiao, Fei Huang, Yu Li, Man Li, Wanjian Liu, Wang Honggang, Fanyang Mo, Dingkai Su, Peng Wang, Yao Yuan, Xuefeng Guo, Guojun Ke, Zhiting Yang, and Yu Zhao
- Subjects
Materials science ,Point-of-care testing ,02 engineering and technology ,biosensor ,010402 general chemistry ,01 natural sciences ,law.invention ,Nucleic acid thermodynamics ,chemistry.chemical_compound ,law ,medicine ,General Materials Science ,immunoassay ,Bifunctional ,Polymerase chain reaction ,Detection limit ,Chromatography ,medicine.diagnostic_test ,nucleic acid detection ,Detector ,COVID-19 ,Articles ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,point-of-care testing ,chemistry ,Immunoassay ,0210 nano-technology ,Biosensor - Abstract
Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread and caused a severe global pandemic. Because no specific drugs are available for COVID-19 and few vaccines are available for SARS-CoV-2, accurate and rapid diagnosis of COVID-19 has been the most crucial measure to control this pandemic. Here, we developed a portable bifunctional electrical detector based on graphene fieldeffect transistors for SARS-CoV-2 through either nucleic acid hybridization or antigen-antibody protein interaction, with ultra-low limits of detection of ~0.1 and ~1 fg mLâ1 in phosphate buffer saline, respectively. We validated our method by assessment of RNA extracts from the oropharyngeal swabs of ten COVID-19 patients and eight healthy subjects, and the IgM/IgG antibodies from serum specimens of six COVID-19 patients and three healthy subjects. Here we show that the diagnostic results are in excellent agreement with the findings of polymerase chain reaction-based optical methods; they also exhibit rapid detection speed (~10 min for nucleic acid detection and ~5 min for immunoassay). Therefore, our assay provides an efficient, accurate tool for high-throughput point-of-care testing. Electronic Supplementary Material Supplementary material is available in the online version of this article at 10.1007/s40843-020-1577-y. more...
- Published
- 2020
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