1. Spectroscopic Analysis of the Interaction between Silver Nanoparticles and Trypsin
- Author
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Li Jiansheng, Min Liu, Luo Li, Xiuyun Sun, Rui Lu, and Yi Li
- Subjects
inorganic chemicals ,Circular dichroism ,Chemistry ,education ,010401 analytical chemistry ,technology, industry, and agriculture ,Nanoparticle ,02 engineering and technology ,Chromophore ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Trypsin ,Photochemistry ,01 natural sciences ,Fluorescence ,Silver nanoparticle ,0104 chemical sciences ,Nanomaterials ,medicine ,0210 nano-technology ,Protein secondary structure ,health care economics and organizations ,Spectroscopy ,medicine.drug - Abstract
The rapid development of nanoparticles (NPs) and their broad applications in medicine caused concerns about biological effects and biosafety. Here, the interaction of silver NPs (Ag NPs) and trypsin is studied with ultravioletvisible spectra, circular dichroism, and fluorescence spectra. Trypsin is exposed to various sizes and concentrations of Ag NPs. The intensities of the trypsin ultraviolet-visible absorption peaks are proportional to the Ag NP concentration and size. In the circular dichroism spectra, there was evidence that Ag NPs affected the secondary structure of trypsin. Fluorescence spectra show that the formation of the protein-nanoparticle complexes alters the protein chromophore chemical environment or structure and quenches its fluorescence. Hence, the extent of Ag NP binding of trypsin depends on both NP concentration and size, and changes the Ag NPs physicochemical properties as well as the trypsin secondary structure. With regard to nanomaterial safety, the interaction of NPs with proteins must be explored further for establishing NP toxicity and design guidance.
- Published
- 2021