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Introduction The wound is defined as an injury of a living tissue, arose due to either an accidental injury like cut of the cells or skin or sometime, maybe broken the tissue, which may cause the anomalies in the cellular ability to exhibit the protective mechanism. Wound healing is process of regaining the integrity of cell structure and layers of the skin. The normal healing process of wounds are categorized in 4 phases which includes hemostatic, inflammatory, fibrolastic and maturation. Purpose The present manuscript embarks on up to date information on different methods being used for wound healing assessment including incision, excision, burn wound and dead space models. The biochemical parameter which acts as wound healing markers are collagen estimation (hydroxyproline), hexosamine, DNA estimation, total protein estimation, wound Index, contraction area, epithelization period and tensile strength etc. Histologic evaluations is also an integral part of wound healing evaluation which, help in evaluating the cellular and matrix detail within the wound. Conclusion At present, molecular approaches are important to heal the wound. For assessment of potential of any herbal drug/synthetic compound, incision wound model, excision wound model, burn wound model and dead space model and their parameter are used.

Background Tridax procumbens(L.) leaves are traditionally used in wound healing by tribal people. The matured leaves are crushed to make a paste and applied on the surface of the wound. Purpose The aim of the present investigation was to evaluate the wound healing activity of ethanolic extract of Tridax procumbens (L.) (EETP) in streptozotocin induced diabetic and non- diabetic laboratory animals. There is no scientific literature, claiming the use of leaves of Tridax procumbens (L.) in diabetic wounds. Study Design The simple ointment base (2.5 % and 5% w/w) of EETP was formulated to evaluate the wound healing potential on diabetic and non-diabetic rats, using incision, excision and burn wound model. Methods In incision wound model, epithelization period, wound index, % contraction area, hydroxyproline content, DNA estimation, total protein and histopathological evaluations were done. In excision wound model, tensile strength was measured. In burn model, contraction rate, wound contraction (%) and epithelization period were evaluated. Results Secondary metabolite as tannin, flavonoids were present. In the excision model, animals treated with 2.5 % and 5% w/w EETP showed significant result in wound contraction, epithelization period and wound index. Hydroxyproline, total protein and DNA concentration in healing tissue were more in comparison to control and as well as standard in diabetic and non- diabetic. The parameters evaluated showed significant response at 2.5 % and 5% w/w EETP. Conclusion Flavonoids and tannin were accountable for wound healing. The findings confirmed the ethanomedicinal claim of Tridax procumbens(L.) in wound healing in diabetic and nondiabetic conditions.