1. Glioblastoma CUSA Fluid Protein Profiling: A Comparative Investigation of the Core and Peripheral Tumor Zones
- Author
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Giovanni Sabatino, Claudia Desiderio, Alessandro Olivi, Andrea Urbani, Diana Valeria Rossetti, Giorgia Antonia Simboli, Giuseppe La Rocca, Tamara Ius, Federica Vincenzoni, and Giuseppe Maria Della Pepa
- Subjects
0301 basic medicine ,Cancer Research ,Settore MED/27 - NEUROCHIRURGIA ,Brain tumor ,CD59 ,Proteomics ,lcsh:RC254-282 ,Article ,CUSA fluid ,Glioblastoma multiforme ,Serine ,03 medical and health sciences ,glioblastoma multiforme ,0302 clinical medicine ,proteomics ,medicine ,Osteopontin ,biology ,CD44 ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Tropomyosin ,030104 developmental biology ,Histone ,Oncology ,biology.protein ,Cancer research ,030217 neurology & neurosurgery ,brain tumor - Abstract
The present investigation aimed to characterize the protein profile of cavitating ultrasound aspirator fluid of newly diagnosed and recurrent glioblastoma comparing diverse zones of collection, i.e., tumor core and tumor periphery, with the aid of 5-aminolevulinic acid fluorescence. The samples were pooled and analyzed in triplicate by LC-MS following the shotgun proteomic approach. The identified proteins were then grouped to disclose elements exclusive and common to the tumor state or tumor zones and submitted to gene ontology classification and pathway overrepresentation analysis. The proteins common to the distinct zones were further investigated by relative quantitation, following a label free approach, to disclose possible differences of expression. Nine proteins, i.e., tubulin 2B chain, CD59, far upstream element-binding, CD44, histone H1.4, caldesmon, osteopontin, tropomyosin chain and metallothionein-2, marked the core of newly diagnosed glioblastoma with respect to tumor periphery. Considering the tumor zone, including the core and the fluorescence positive periphery, the serine glycine biosynthesis, pentose phosphate, 5-hydroxytryptamine degredation, de novo purine biosynthesis and huntington disease pathways resulted statistically significantly overrepresented with respect to the human genome of reference. The fluorescence negative zone shared several protein elements with the tumor zone, possibly indicating the presence of pathological aspects of glioblastoma rather than of normal brain parenchyma. On the other hand, its exclusive protein elements were considered to represent the healthy zone and, accordingly, exhibiting no pathways overrepresentation. On the contrary to newly diagnosed glioblastoma, pathway overrepresentation was recognized only in the healthy zone of recurrent glioblastoma. The TGF&beta, signaling pathway, exclusively classified in the fluorescence negative periphery in newly diagnosed glioblastoma, was instead the exclusive pathway classified in the tumor core of recurrent glioblastoma. These results, preliminary obtained on sample pools, demonstrated the potential of cavitron ultrasonic sur
- Published
- 2021