1. Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii
- Author
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Byung Soon Hwang, Yoon-Ju Lee, Seung Woong Lee, In-Kyoung Lee, Dae-Won Kim, Ji-Yul Kim, E-Eum Woo, Bong-Sik Yun, and Ja-Gyeong Song
- Subjects
0301 basic medicine ,Mushroom ,Inoscavin A ,Collybia ,biology ,Traditional medicine ,010405 organic chemistry ,Korean native mushroom ,Fractionation ,Phellinus gilvus ,biology.organism_classification ,Inhibitory postsynaptic potential ,01 natural sciences ,Microbiology ,0104 chemical sciences ,Research Note ,Phellinus baumii ,03 medical and health sciences ,Korean Native ,Lipoxygenase ,030104 developmental biology ,Infectious Diseases ,Lipoxygenase inhibitor ,biology.protein ,IC50 - Abstract
We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 µg/mL. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an IC50 value of 6.8 µM.
- Published
- 2014