1. Development of Spherical Nucleic Acids for Prostate Cancer Immunotherapy
- Author
-
Lei Qin, Shuya Wang, Donye Dominguez, Alan Long, Siqi Chen, Jie Fan, Jihae Ahn, Kacper Skakuj, Ziyin Huang, Andrew Lee, Chad Mirkin, and Bin Zhang
- Subjects
Cytotoxicity, Immunologic ,Male ,0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,medicine.medical_treatment ,Immunology ,Lymphocyte Activation ,Cancer Vaccines ,Mice ,03 medical and health sciences ,Prostate cancer ,Cross-Priming ,0302 clinical medicine ,Antigen ,Cancer immunotherapy ,Antigens, Neoplasm ,CpG ,medicine ,Animals ,Humans ,Immunology and Allergy ,Cytotoxic T cell ,Original Research ,Chemistry ,Prostatic Neoplasms ,Immunotherapy ,vaccines ,medicine.disease ,prostate cancer ,Nanostructures ,Mice, Inbred C57BL ,030104 developmental biology ,Oligodeoxyribonucleotides ,CpG site ,Immunostimulatory Spherical Nucleic Acids (IS-SNAs) ,Spherical nucleic acid ,Cancer research ,immunotherapy ,lcsh:RC581-607 ,Adjuvant ,T-Lymphocytes, Cytotoxic ,030215 immunology - Abstract
Although the strategy of therapeutic vaccination for the treatment of prostate cancer has advanced to and is available in the clinic (Sipuleucel-T), the efficacy of such therapy remains limited. Here, we develop Immunostimulatory Spherical Nucleic Acid (IS-SNA) nanostructures comprised of CpG oligonucleotides as adjuvant and prostate cancer peptide antigens, and evaluate their antitumor efficacy in syngeneic mouse models of prostate cancer. IS-SNAs with the specific structural feature of presenting both antigen and adjuvant CpG on the surface (hybridized model (HM) SNAs) induce stronger cytotoxic T lymphocyte (CTL) mediated antigen-specific killing of target cells than that for IS-SNAs with CpG on the surface and antigen encapsulated within the core (encapsulated model (EM) SNAs). Mechanistically, HM SNAs increase the co-delivery of CpG and antigen to dendritic cells over that for EM SNAs or admixtures of linear CpG and peptide, thereby improving cross-priming of antitumor CD8+ T cells. As a result, vaccination with HM SNAs leads to more effective antitumor immune responses in two prostate cancer models. These data demonstrate the importance of the structural positioning of peptide antigens together with adjuvants within IS-SNAs to the efficacy of IS-SNA-based cancer immunotherapy.
- Published
- 2020