1. Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil
- Author
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Vanessa dos Santos Morais, Eric Delwart, Ramendra Pati Pandey, Danielle Elise Gill, Maurício Lacerda Nogueira, Sirle Abdo Salloum Scandar, Antonio Charlys da Costa, Ester Cerdeira Sabino, Paulo César Maiorka, V. Samuel Raj, Renato Pereira de Souza, Roozbeh Tahmasebi, Xutao Deng, Juliana Silva Nogueira, Leandro Guaraglia D'Agostino, Mariana Sequetin Cunha, Élcio Leal, Fernanda G. S. Vasami, Edcelha Soares D’Athaide Ribeiro, Marlisson Octavio da S Rego, Fabiola Villanova, Geovani de Oliveira Ribeiro, Adriana Yurika Maeda, and Fred Julio Costa Monteiro
- Subjects
0301 basic medicine ,Science ,viruses ,030106 microbiology ,FILOGENIA ,Genome, Viral ,Mosquito Vectors ,complex mixtures ,Article ,Evolutionary genetics ,Virus ,Cell Line ,Dengue fever ,Evolution, Molecular ,03 medical and health sciences ,Species Specificity ,Aedes ,Virology ,Gene duplication ,medicine ,Animals ,Humans ,3' Untranslated Regions ,Phylogeny ,Multidisciplinary ,Base Sequence ,biology ,Flavivirus ,virus diseases ,RNA ,Genomics ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease ,030104 developmental biology ,Codon usage bias ,Medicine ,RNA, Viral ,Host adaptation - Abstract
Classical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative dISFV, termed Guapiaçu virus (GUAPV). Distinct strains of GUAPV were isolated from pools of Aedes terrens and Aedes scapularis mosquitoes. Additionally, we also detected viral GUAPV RNA in a plasma sample of an individual febrile from the Amazon region (North of Brazil). Although GUAPV did not replicate in tested mammalian cells, 3′UTR secondary structures duplication and codon usage index were similar to pathogenic flavivirus.
- Published
- 2021
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