3 results on '"Livia Maria Silva Moura"'
Search Results
2. Frontiers in Microbiology
- Author
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Aline Maria Da Silva, Roberta Amorim Pereira, João C. Setubal, Luciana Principal Antunes, Layla Farage Martins, Leandro Nascimento Lemos, Livia Maria Silva Moura, Amanda Ribeiro Martins da Silva, and Ronaldo Bento Quaggio
- Subjects
0301 basic medicine ,Microbiology (medical) ,CAZy ,ENZIMAS HIDROLÍTICAS ,consortium ,Computational biology ,engineering.material ,Biology ,Genome ,Microbiology ,metagenome ,Clostridia ,bacterial genome reconstruction ,03 medical and health sciences ,Geobacillus thermoglucosidasius ,Original Research ,Phylogenetic tree ,Compost ,business.industry ,Paenibacillaceae ,thermophilic ,biology.organism_classification ,cellulolytic ,Biotechnology ,030104 developmental biology ,Metagenomics ,engineering ,composting ,glycoside hydrolases ,business - Abstract
Microbial consortia selected from complex lignocellulolytic microbial communities are promising alternatives to deconstruct plant waste, since synergistic action of different enzymes is required for full degradation of plant biomass in biorefining applications. Culture enrichment also facilitates the study of interactions among consortium members, and can be a good source of novel microbial species. Here, we used a sample from a plant waste composting operation in the Sao Paulo Zoo (Brazil) as inoculum to obtain a thermophilic aerobic consortium enriched through multiple passages at 60°C in carboxymethylcellulose as sole carbon source. The microbial community composition of this consortium was investigated by shotgun metagenomics and genome-centric analysis. Six near-complete (over 90%) genomes were reconstructed. Similarity and phylogenetic analyses show that four of these six genomes are novel, with the following hypothesized identifications: a new Thermobacillus species; the first Bacillus thermozeamaize genome (for which currently only 16S sequences are available) or else the first representative of a new family in the Bacillales order; the first representative of a new genus in the Paenibacillaceae family; and the first representative of a new deep-branching family in the Clostridia class. The reconstructed genomes from known species were identified as Geobacillus thermoglucosidasius and Caldibacillus debilis. The metabolic potential of these recovered genomes based on COG and CAZy analyses show that these genomes encode several glycoside hydrolases (GHs) as well as other genes related to lignocellulose breakdown. The new Thermobacillus species stands out for being the richest in diversity and abundance of GHs, possessing the greatest potential for biomass degradation among the six recovered genomes. We also investigated the presence and activity of the organisms corresponding to these genomes in the composting operation from which the consortium was built, using compost metagenome and metatranscriptome datasets generated in a previous study. We obtained strong evidence that five of the six recovered genomes are indeed present and active in that composting process. We have thus discovered three (perhaps four) new thermophillic bacterial species that add to the increasing repertoire of known lignocellulose degraders, whose biotechnological potential can now be investigated in further studies.
- Published
- 2017
3. Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics
- Author
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Luciano Antonio Digiampietri, Aline Maria Da Silva, Julio Cezar Franco de Oliveira, Gianluca Major Machado Silva, Karen Cristina Lombardi, Patrícia Locosque Ramos, Leandro Nascimento Lemos, Andrew Maltez Thomas, João Batista da Cruz, George Willian Condomitti Epamino, Layla Farage Martins, Renata C. Pascon, Deibs Barbosa, Ronaldo Bento Quaggio, Livia Maria Silva Moura, Luciana Principal Antunes, Roberta Verciano Pereira, and João C. Setubal
- Subjects
0301 basic medicine ,Population ,Microbial Consortia ,Biomass ,010501 environmental sciences ,engineering.material ,Biology ,01 natural sciences ,complex mixtures ,Lignin ,Article ,03 medical and health sciences ,RNA, Ribosomal, 16S ,education ,Soil Microbiology ,0105 earth and related environmental sciences ,education.field_of_study ,Multidisciplinary ,Bacteria ,Compost ,Ecology ,Thermophile ,Composting ,Gene Expression Profiling ,High-Throughput Nucleotide Sequencing ,MYCOBACTERIUM ,Biodegradation ,030104 developmental biology ,Biodegradation, Environmental ,Microbial population biology ,Metagenomics ,engineering ,Soil microbiology - Abstract
Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomic- and metatranscriptomic-based analyses of a large composting operation in the São Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 °C to 75 °C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.
- Published
- 2016
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