1. Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture
- Author
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Hitoshi Okamura, Masao Doi, Motomi Tainaka, Iori Murai, and Yuichi Inoue
- Subjects
0301 basic medicine ,endocrine system ,Physiology ,CLOCK Proteins ,Endogeny ,Cycloheximide ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Physiology (medical) ,Circadian Clocks ,Animals ,Circadian rhythm ,Cells, Cultured ,Translation (biology) ,Period Circadian Proteins ,Fibroblasts ,Embryonic stem cell ,Cell biology ,Circadian Rhythm ,PER2 ,030104 developmental biology ,chemistry ,Phosphorylation ,Suprachiasmatic Nucleus - Abstract
It is not known whether the endogenous mammalian core clock proteins sustain measurable oscillations in cells in culture where de novo translation is pharmacologically inhibited. We studied here the mammalian core clock protein PER2, which undergoes robust circadian oscillations in both abundance and phosphorylation. With a newly developed antibody that enables tracing the endogenous PER2 protein oscillations over circadian cycles with cultured mouse embryonic fibroblast cells, we provide evidence that PER2 does not persist noticeable circadian rhythms when translation is inhibited.
- Published
- 2017