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17 results on '"Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse]"'

1. The Multifaceted Interplay between Atrial Fibrillation and Myocardial Infarction: A Review

Author

Hanon, Olivier, Vidal, Jean-Sébastien, Pisica-Donose, George, Orvoën, Galdric, David, Jean-Philippe, Chaussade, Edouard, Caillard, Laure, de Jong, Laura, Boulloche, Nicolas, Vinsonneau, Ulric, Bouée, Stéphane, Krolak-Salmon, Pierre, Fauchier, Laurent, Jouanny, Pierre, Sacco, Guillaume, Bellarbre, Fabienne, Belmin, Joël, Puisieux, François, Lilamand, Matthieu, Paillaud, Elena, Boureau, Anne Sophie, Belkouche, Alban, Yao, Hermann, Putot, Alain, Chagué, Frédéric, Rochette, Luc, Danchin, Nicolas, Zeller, Marianne, Cottin, Yves, AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Cité (UPC), Maladie d'Alzheimer : marqueurs génétiques et vasculaires, neuropsychologies (URP_4468), Groupe hospitalier Broca-Université Paris Cité (UPC), DomusVi [Suresnes] (Maisons de retraite), Hôpital Vaugirard-Gabriel Pallez, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse], Service de Cardiologie (HIA BREST - Cardio), HIA - BREST, Pôle Real World Evidence [Bourg-la-Reine] (CEMKA), Cemka-eval [Bourg La Reine], Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Elderly Health Service, Hôpital des Charpennes [CHU - HCL], Hospices Civils de Lyon (HCL), CHU Trousseau [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Éducation Éthique Santé EA 7505 (EES), Université de Tours (UT), Service de médecine gériatrique (CHU de Dijon - Centre gériatrique de Champmaillot - EHPAD), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Laboratoire de Psychologie des Pays de la Loire (LPPL), Université d'Angers (UA)-Université de Nantes - UFR Lettres et Langages (UFRLL), Université de Nantes (UN)-Université de Nantes (UN), CHU Charles Foix [AP-HP], Pôle de gérontologie [CHRU de Lille], Hôpital Roger Salengro [Lille]-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre de Neurologie Cognitive [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpitaux Universitaires Saint-Louis, Lariboisière, Fernand-Widal, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Hôpital Européen Georges Pompidou [APHP] (HEGP), Service de Cardiologie [CHU de Dijon], Physiopathologie et épidémiologie cérébro-cardiovasculaire [Dijon] (PEC2), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC), European Atherosclerosis Society [Göteborg, Sweden] (EAS), and Dorogoichenko, Aleksandra

Subjects
medicine.medical_specialty, lcsh:Medicine, Review, 030204 cardiovascular system & hematology, Coronary artery embolism, [SHS]Humanities and Social Sciences, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Hospital discharge, atrial fibrillation, 030212 general & internal medicine, Myocardial infarction, Excess mortality, chronic coronary syndrome, business.industry, lcsh:R, Atrial fibrillation, General Medicine, medicine.disease, bleeding, Coronary arteries, medicine.anatomical_structure, type 1 and type 2 myocardial infarction, Cardiology, [SHS] Humanities and Social Sciences, business, Ischemic heart
Abstract

International audience; This review was conducted to emphasize the complex interplay between atrial fibrillation (AF) and myocardial infraction (MI). In type 1 (T1) MI, AF is frequent and associated with excess mortality. Moreover, AF after hospital discharge for T1MI is not rare, suggesting the need to improve AF screening and to develop therapeutic strategies for AF recurrence. Additionally, AF is a common trigger for type 2 MI (T2MI), and recent data have shown that tachyarrhythmia or bradyarrhythmia could be a causal factor in, respectively, 13–47% or 2–7% of T2MI. In addition, AF is involved in T2MI pathogenesis as a result of severe anemia related to anticoagulants. AF is also an underestimated and frequent cause of coronary artery embolism (CE), as a situation at risk of myocardial infarction with non-obstructive coronary arteries. AF-causing CE is difficult to diagnose and requires specific management. Moreover, patients with both AF and chronic coronary syndromes represent a therapeutic challenge because the treatment of AF include anticoagulation, depending on the embolic risk, and ischemic heart disease management paradoxically includes antiplatelet therapy.

Published
2021

2. HIHARS (« hyperventilation-induced high-amplitude rhythmic slowing ») : EEG à ne pas confondre avec une absence épileptique

Author

L. Vercueil, C. Bruneau, Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
03 medical and health sciences, 0302 clinical medicine, business.industry, 030225 pediatrics, [SDV]Life Sciences [q-bio], Medicine, Neurology (clinical), business, 030217 neurology & neurosurgery
Published
2020

3. Do sensorial manipulations affect subjects differently depending on their postural abilities?

Author

Noé, Frédéric, Bernard, Nicolas, Hazard, Clement, Paillard, Thierry, Bizid, R., Dupui, Philippe, Mouvement, Équilibre, Performance, Santé (MEPS), Université de Pau et des Pays de l'Adour (UPPA), Géoarchitecture : Territoires, Urbanisation, Biodiversité, Environnement, Université de Rennes (UR)-Université de Brest (UBO), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Brest (UBO)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
Adult, Male, medicine.medical_specialty, Posture, Physical Therapy, Sports Therapy and Rehabilitation, Sensory system, Affect (psychology), Postural control, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Soccer, medicine, Humans, Orthopedics and Sports Medicine, Force platform, Foot pressure, ComputingMilieux_MISCELLANEOUS, Balance (ability), Proprioception, 030229 sport sciences, General Medicine, Electric Stimulation, Football soccer, Physical therapy, Original Article, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Psychology, human activities, 030217 neurology & neurosurgery
Abstract

Objectives: To examine whether sensorial manipulation affects subjects differently according to their postural performance and the strategies used. The literature showed that the level of competition of soccer players influences their postural performance and strategy. Methods: Eight high-level (HL) professional soccer players and nine regional-level (RL) soccer players were tested (1) in a reference condition and (2) in a manipulated sensorial condition (MAN). The MAN condition consisted of perturbing the proprioceptive and exteroceptive information. For each postural condition, balance was assessed by measuring the centre of foot pressure using a force platform during a test of bipedal standing posture. Results: The postural control was less perturbed in the HL than in the RL players in the two postural conditions. Moreover, the group–condition interaction showed that the postural control was less disturbed in the HL than in the RL players when the sensory information was manipulated. Conclusions: The HL soccer players probably possessed a better internal model of verticality than the RL players. Subjects who had a better postural control level were less disturbed by sensorial manipulation than the others in postural regulation.

Published
2007

4. Bilateral Wada test: Amobarbital or propofol?

Author

Marie Denuelle, Luc Valton, Jonathan Curot, M. Kany, Thomas Busigny, P. Tall, Nelly Fabre, F. Marlat, Gladys Barragan-Jason, Centre de recherche cerveau et cognition (CERCO), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
Adult, Male, medicine.medical_specialty, Adolescent, Consciousness, Amobarbital, Clinical Neurology, Neuropsychological Tests, Wada, Functional Laterality, 030218 nuclear medicine & medical imaging, Temporal lobe, Young Adult, 03 medical and health sciences, Epilepsy, 0302 clinical medicine, Memory, Preoperative Care, medicine, Humans, Epilepsy surgery, Adverse effect, Propofol, Anesthetics, Language, Retrospective Studies, medicine.diagnostic_test, [SCCO.NEUR]Cognitive science/Neuroscience, Brain, General Medicine, Gold standard (test), Middle Aged, medicine.disease, Surgery, Epilepsy, Temporal Lobe, Neurology, Anesthesia, Visual Perception, Wada test, Female, Neurology (clinical), Psychology, Temporal epilepsy, 030217 neurology & neurosurgery, medicine.drug
Abstract

A B S T R A C T Purpose: The Wada test is still the gold standard procedure to predict language and memory deficits before temporal lobe epilepsy surgery. As amobarbital was no longer available, our aim was to validate propofol as an alternative. Method: We retrospectively studied 47 patients who underwent a bilateral intracarotid procedure, performed with amobarbital (18), or propofol (29), between 2000 and 2010 during the preoperative evaluation of temporal lobe epilepsy. Results: The number of patients experiencing an adverse event (mostly transient disturbance of consciousness or benign ocular symptoms) during both injections did not differ significantly between amobarbital and propofol. Hemispheric dominance was successfully determined in 96.5% patients with propofol vs. 94.4% with amobarbital for language, and in 72.4% under propofol vs. 77.7% under amobarbital for memory with no significant difference between groups. Conclusion: Propofol can be used for the Wada test with an efficacy and safety comparable to amobarbital.

Published
2014

5. Between-day reliability of centre of pressure measures for balance assessment in hemiplegic stroke patients

Author

David Amarantini, Philippe Dupui, Marc Labrunee, Richard Montoya, David Gasq, Philippe Marque, CHU Toulouse [Toulouse], Programme Interdisciplinaire de Recherche en Sciences du Sport et du Mouvement Humain (PRISSMH), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Equipe 7 Inserm U1048, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Rééducation Cardiovasculaire, CHU Toulouse [Toulouse]-Hôpital de Rangueil, Imagerie cérébrale et handicaps neurologiques (ICHN), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Montréal (UdeM), Hôpital de Rangueil, BMC, Ed., Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service Cardiologie [CHU Toulouse], Pôle Cardiovasculaire et Métabolique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Département de kinésiologie, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service Médecine physique et de réadaptation [CHU Toulouse], Pôle Neurosciences [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Santé publique et médecine publique [CHU Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, Service de Médecine Physique et Réadaptation, and GASQ, David

Subjects
Male, Reproducibility of results, 030506 rehabilitation, medicine.medical_specialty, Intraclass correlation, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Health Informatics, Hemiplegia, Heteroscedasticity, Standard deviation, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Bias, medicine, Postural Balance, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, Force platform, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Centre of Pressure (CoP), Stroke, Reliability (statistics), Neurologic Examination, Observational error, Research, Posturography, Rehabilitation, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Middle Aged, medicine.disease, Postural balance, Physical therapy, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], 0305 other medical science, Psychology, 030217 neurology & neurosurgery
Abstract

International audience; BACKGROUND: Stroke patients have impaired postural balance that increases the risk of falls and impairs their mobility. Assessment of postural balance is commonly carried out by recording centre of pressure (CoP) displacements, but the lack of data concerning reliability of these measures compromises their interpretation. The purpose of this study was to investigate the between-day reliability of six CoP-based variables, in order to provide i) reliability data for monitoring postural sway and weight-bearing asymmetry of stroke patients in clinical practice and ii) consistent assessment method of measurement error for applications in physical medicine and rehabilitation. METHODS: Postural balance of 20 stroke patients was assessed in quiet standing on a force platform, in two sessions, 7 days apart. Six CoP-based variables were collected in eyes open and eyes closed conditions: postural sway was assessed with mean and standart deviation of CoP-velocity, CoP-velocity along the mediolateral and anteroposterior axes, and confidence ellipse area (CE(AREA)); weight-bearing asymmetry was assessed with mean CoP position along the mediolateral axis (CoP(ML)). The intraclass correlation coefficient (ICC) was used to determine the level of agreement between test-retest. Small real difference (SRD), corresponding to the smallest change that indicates a real improvement for a single individual, was used to determine the extent of measurement error. RESULTS: ICCs were satisfactory (>0.9) for all CoP-based variables, except for CE(AREA) in eyes open condition and CoP(ML) (

Published
2014

6. Memory monitoring and memory control in patients suffering from obstructive sleep apnoea syndrome

Author

Michel Tiberge, Agnès Daurat, Nathalie Huet, Cognition, Langues, Langage, Ergonomie (CLLE-LTC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Toulouse - Jean Jaurès (UT2J)-Centre National de la Recherche Scientifique (CNRS), University of Toulouse 2, France, Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
Male, medicine.medical_specialty, Memory control, Disorders of Excessive Somnolence, Audiology, Anxiety, behavioral disciplines and activities, 050105 experimental psychology, Task (project management), 03 medical and health sciences, Judgment, 0302 clinical medicine, Arts and Humanities (miscellaneous), medicine, Memory impairment, Humans, 0501 psychology and cognitive sciences, In patient, Psychiatry, General Psychology, Obstructive sleep apnoea syndrome, ComputingMilieux_MISCELLANEOUS, Memory Disorders, Sleep Apnea, Obstructive, Recall, Depression, [SCCO.NEUR]Cognitive science/Neuroscience, 05 social sciences, Recall test, Impaired memory, Middle Aged, Paired-Associate Learning, Mental Recall, [SCCO.PSYC]Cognitive science/Psychology, Female, Psychology, 030217 neurology & neurosurgery
Abstract

Patients with obstructive sleep apnoea syndrome exhibit memory deficit. The present study looked at whether this deficit is related to impaired memory monitoring and/or memory control. Here 25 patients and 26 healthy controls performed a paired-associate learning task. After participants had made a judgement of learning for each pair and performed an initial recall test they were free to restudy any items they wished, for as long or little as they wished, within a 5-minute period. They then performed a second recall test. Monitoring and control processes were assessed on the basis of judgements of learning, item selection, and study-time allocation. In spite of their memory impairment, patients accurately predicted their recall. For the restudy phase patients preferentially selected the judged-easy items, while controls selected the judged-difficult items. However, all the participants allocated more restudy time to the judged-difficult items than to the judged-easy ones. There were no significant correlations between memory performance, metamemory processes, and clinical measures (i.e., subjective sleepiness, subjective sleep quality, anxiety, and depression scores). Results suggested that both memory monitoring and memory control were preserved in our sample of patients with obstructive sleep apnoea.

Published
2013

7. Gender as a Modifying Factor Influencing Myotonic Dystrophy Type 1 Phenotype Severity and Mortality: A Nationwide Multiple Databases Cross-Sectional Observational Study

Author

Hugo Varet, Guilhem Solé, Dominique Menard, Celia Boutte, Khadija Amarof, Valerie Drouin-Garraud, Jack Puymirat, Gerard Riviere, Felix Rougier, Françoise Chapon, Marguerite Preudhomme, Anne-Laure Bedat-Millet, Xavier Ferrer, Dalil Hamroun, Tanya Stojkovich, Vincent Tiffreau, Pascal Cintas, Romain K. Gherardi, Laurent Magy, Céline Dogan, Karima Ghorab, Françoise Bouhour, Marianne Fabbro, Sybille Pellieux, Gilbert Caranhac, Romain Deschamps, Rémi Bellance, Helene Gervais-Bernard, Pascal Laforêt, Marie-Christine Minot, Sabrina Sacconi, Emmanuelle Campana-Salort, Marie-Christine Arne Bes, Claude Desnuelle, Aleksandra Nadaj-Pakleza, Andoni Urtizberea, Christophe Vial, François Boyer, Anthony Behin, Armelle Magot, Fabien Zagnoli, Bruno Eymard, Yann Péréon, Jean Pouget, Guillaume Bassez, Marie De Antonio, Sandrine Katsahian, Alain Geille, Claude Bourlier, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5), Laboratoire de génétique des maladies rares. Pathologie moleculaire, etudes fonctionnelles et banque de données génétiques (LGMR), Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), CHU de Fort de France, Centre de Reference des Maladies Neuromusculaires - Atlantique Occitanie Caraïbe (CRMN - AOC), CHU Rouen, Normandie Université (NU), Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU de la Martinique [Fort de France], Groupement Hospitalier Est, Centre Hospitalier Universitaire [Grenoble] (CHU), Santé publique, vieillissement, qualité de vie et réadaptation des sujets fragiles - EA 3797 (SPVQVRSF), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Service de Médecine Physique Neurologique-Traumatologique [Reims], Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Hôpital Sébastopol, Génétique Médicale et Génomique Fonctionnelle (GMGF), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de référence des maladies neuromusculaires et de la SLA, Hôpital de la Timone [CHU - APHM] (TIMONE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Mobilités : Attention, Orientation et Chronobiologie (COMETE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire de Nice (CHU Nice), CHU Bordeaux [Bordeaux], Service de Neurologie [CHU Limoges], CHU Limoges, Centre de référence national neuropathies périphériques rares [CHU Limoges], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire d'Explorations Fonctionnelles, Centre hospitalier universitaire de Nantes (CHU Nantes), Centre de Référence des Maladies Neuromusculaires Rares de l'Enfant et de l'Adulte Nantes-Angers, Maintenance Myélinique et Neuropathies Périphériques (MMNP), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), CHU Pontchaillou [Rennes], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), CHU Trousseau [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Hôtel-Dieu de Nantes, Université de Nantes (UN), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Neuro-Oncologie [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Hôpital Marin, Hôpital neurologique et neurochirurgical Pierre Wertheimer [CHU - HCL], Hospices Civils de Lyon (HCL), Hôpital d'Instruction des Armées Clermont Tonnerre, Service de Santé des Armées, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Jonchère, Laurent, Centre de référence des maladies rares neuromusculaires Aquitaine-Grand Sud Ouest, CHU Toulouse [Toulouse], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA), Univ. Littoral Côte d’Opale, Univ. Artois, Université de Lille, Université Paris-Est Créteil Val-de-Marne - Paris 12 [UPEC UP12], CHU Montpellier, Hôpital Pierre Zobda-Quitman [CHU de la Martinique], Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Centre Hospitalier Universitaire [Grenoble] [CHU], Centre Hospitalier Universitaire de Reims [CHU Reims], Hôpital de la Timone [CHU - APHM] [TIMONE], Centre Hospitalier Universitaire de Nice [CHU Nice], Centre hospitalier universitaire de Nantes [CHU Nantes], Centre Hospitalier Universitaire d'Angers [CHU Angers], Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille], Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 [URePSSS], Association française contre les myopathies [AFM-Téléthon], and Université Pierre et Marie Curie - Paris 6 [UPMC]

Subjects
Male, 0301 basic medicine, Databases, Factual, Physiology, Cross-sectional study, Myotonic dystrophy, lcsh:Medicine, Disease, computer.software_genre, Laryngology, 0302 clinical medicine, Medicine and Health Sciences, Ethnicities, Medicine, French People, lcsh:Science, education.field_of_study, Multidisciplinary, Death rates, Database, Cognitive Neurology, Mortality rate, Dysphagia, 3. Good health, Phenotype, Cognitive impairment, Neurology, Physiological Parameters, Female, Research Article, Adult, Maternal inheritance, Cognitive Neuroscience, Population, [SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics, 03 medical and health sciences, Population Metrics, Adults, Humans, Obesity, Sex Distribution, education, Demography, Population Biology, Cataracts, business.industry, lcsh:R, Body Weight, Biology and Life Sciences, medicine.disease, Myotonia, Thyroid disorder, Health Care, Ophthalmology, Cross-Sectional Studies, 030104 developmental biology, Otorhinolaryngology, Socioeconomic Factors, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Age Groups, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Lens Disorders, People and Places, Cognitive Science, lcsh:Q, Population Groupings, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Health Statistics, Morbidity, Age of onset, business, computer, 030217 neurology & neurosurgery, Neuroscience
Abstract

International audience; BACKGROUND: Myotonic Dystrophy type 1 (DM1) is one of the most heterogeneous hereditary disease in terms of age of onset, clinical manifestations, and severity, challenging both medical management and clinical trials. The CTG expansion size is the main factor determining the age of onset although no factor can finely predict phenotype and prognosis. Differences between males and females have not been specifically reported. Our aim is to study gender impact on DM1 phenotype and severity.METHODS: We first performed cross-sectional analysis of main multiorgan clinical parameters in 1409 adult DM1 patients (\textgreater18y) from the DM-Scope nationwide registry and observed different patterns in males and females. Then, we assessed gender impact on social and economic domains using the AFM-Téléthon DM1 survey (n = 970), and morbidity and mortality using the French National Health Service Database (n = 3301). RESULTS: Men more frequently had (1) severe muscular disability with marked myotonia, muscle weakness, cardiac, and respiratory involvement; (2) developmental abnormalities with facial dysmorphism and cognitive impairment inferred from low educational levels and work in specialized environments; and (3) lonely life. Alternatively, women more frequently had cataracts, dysphagia, digestive tract dysfunction, incontinence, thyroid disorder and obesity. Most differences were out of proportion to those observed in the general population. Compared to women, males were more affected in their social and economic life. In addition, they were more frequently hospitalized for cardiac problems, and had a higher mortality rate.CONCLUSION: Gender is a previously unrecognized factor influencing DM1 clinical profile and severity of the disease, with worse socio-economic consequences of the disease and higher morbidity and mortality in males. Gender should be considered in the design of both stratified medical management and clinical trials

Published
2016

8. A PET study of photophobia during spontaneous migraine attacks

Author

Marie Denuelle, Yves Trotter, N. Boulloche, Nelly Fabre, Gilles Géraud, Pierre Payoux, Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Imagerie cérébrale et handicaps neurologiques (ICHN), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche cerveau et cognition (CERCO), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Analgesics, Photophobia, genetic structures, MESH: Migraine without Aura, 03 medical and health sciences, 0302 clinical medicine, MESH: Analysis of Variance, medicine, otorhinolaryngologic diseases, Ictal, 030304 developmental biology, 0303 health sciences, MESH: Visual Pathways, MESH: Humans, MESH: Trigeminal Nerve, MESH: Visual Cortex, MESH: Injections, Subcutaneous, MESH: Adult, MESH: Sumatriptan, Visual snow, MESH: Cerebrovascular Circulation, medicine.disease, eye diseases, MESH: Positron-Emission Tomography, MESH: Male, Visual field, Sumatriptan, Visual cortex, medicine.anatomical_structure, Nociception, MESH: Photic Stimulation, Migraine, Anesthesia, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Neurology (clinical), medicine.symptom, MESH: Photophobia, Psychology, MESH: Female, 030217 neurology & neurosurgery, medicine.drug
Abstract

Background: Photophobia is an abnormal sensitivity to light experienced by migraineurs during attacks. The pathophysiology of photophobia is poorly understood. Nevertheless, 2 facts appear to have a link with photophobia: visual cortex hyperexcitability on the one hand and interactions between visual pathway and trigeminal nociception on the other. Methods: We used H 2 15 O PET to study photophobia induced by continuous luminous stimulation covering the whole visual field in 8 migraineurs during spontaneous migraine attacks, after headache relief by sumatriptan and during attack-free interval. The intensity of the luminous stimulation provoking photophobia with subsequent headache enhancement was specifically determined for each patient. Results: We found that low luminous stimulation (median of 240 Cd/m 2 ) activated the visual cortex during migraine attacks and after headache relief but not during the attack-free interval. The visual cortex activation was statistically stronger during migraine headache than after pain relief. Conclusion: These findings suggest that ictal photophobia is linked with a visual cortex hyperexcitability. The mechanism of this cortical hyperexcitability could not be explained only by trigeminal nociception because it persisted after headache relief. We hypothesize that modulation of cortical excitability during migraine attack could be under brainstem nuclei control.

Published
2011

9. Neural networks underlying parietal lobe seizures: a quantified study from intracerebral recordings

Author

Martine Gavaret, Fabrice Wendling, Russell Hewett, Jean Régis, Fabrice Bartolomei, Patrick Chauvel, Luc Valton, Sandrine Aubert, Service de Neurophysiologie Clinique, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Epilepsies, Lesions Cerebrales et Systemes Neuraux de la Cognition, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Male, Unconsciousness, Electroencephalography, Functional Laterality, MESH: Magnetic Resonance Imaging, Epilepsy, 0302 clinical medicine, MESH: Child, Parietal Lobe, Epilepsy surgery, Child, Psychomotor Agitation, 0303 health sciences, MESH: Parietal Lobe, MESH: Middle Aged, medicine.diagnostic_test, Parietal lobe, MESH: Psychomotor Agitation, Middle Aged, MESH: Seizures, Magnetic Resonance Imaging, Neurology, Vestibular Diseases, MESH: Young Adult, MESH: Epilepsy, Cardiology, [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Female, Psychology, MESH: Vestibular Diseases, Algorithms, Adult, medicine.medical_specialty, Adolescent, SEEG, Posterior parietal cortex, MESH: Algorithms, Superior parietal lobule, MESH: Unconsciousness, Stereoelectroencephalography, 03 medical and health sciences, Young Adult, Seizures, Internal medicine, MESH: Electroencephalography, medicine, Humans, Partial epilepsy, Rapid discharge, MESH: Functional Laterality, 030304 developmental biology, MESH: Adolescent, MESH: Humans, Inferior parietal lobule, MESH: Adult, medicine.disease, MESH: Male, Epileptogenicity index, MESH: Nerve Net, Neurology (clinical), Nerve Net, Neuroscience, MESH: Female, 030217 neurology & neurosurgery
Abstract

International audience; In this study we have quantified the "epileptogenicity" of several brain regions in seizures originating in the posterior parietal cortex in 17 patients investigated by intracerebral recordings using stereotactic EEG (SEEG). Epileptogenicity of brain structures was quantified according to the "epileptogenicity index" (EI), a way to quantify rapid discharges at seizure onset. Seven patients had maximal epileptogenicity in the superior parietal lobule-BA area 7 (Gr1), 2 patients in the superior parietal lobule-area 5 (Gr2), 4 patients in inferior parietal lobule (Gr3) and 4 in the opercular region (Gr4). A large majority of patients (15/17 (88%)) reported to have at least one aura during the course of their disease. Somato-sensory manifestations were reported in the four groups. Vestibular disturbance was observed mainly in seizures from the superior parietal lobule (Gr1 and 2). Ipsilateral version was the most frequent objective manifestation (64%). Hyperkinetic behaviour (motor agitation) was found to be frequent, observed in 4/17 cases (23%) and observed in seizures from inferior parietal regions. In conclusion, the electrophysiological organization and the clinical manifestations of PLS are various and complex. The subjective manifestations are frequent and often suggestive, therefore must be actively sought.

Published
2010

10. Genotype-Phenotype Correlations in Charcot-Marie-Tooth Disease Type 2 Caused by Mitofusin 2 Mutations

Author

Marie-Christine Arne-Bes, Franck Sturtz, Corinne Magdelaine, Benoît Funalot, Brigitte Gilbert-Dussardier, Jean-Michel Vallat, Judith Calvo, Annick Toutain, Laurent Magy, Karima Ghorab, Marie-Christine Minot-Myhie, Claire Guillou, Jean-Pierre Carrière, Robert A. Ouvrier, Pierre Bouche, Philippe De Mas, Hubert Journel, Leila Lazaro, Service de Neurologie [CHU Limoges], CHU Limoges, Centre de référence national neuropathies périphériques rares [CHU Limoges], Intituto de neurologia, Universidad de la República [Montevideo] (UCUR), Biomolécules Thérapies anti-tumorales (EA4021), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Service de Biochimie et Génétique Moléculaire [CHU Limoges], Institute of Neuromuscular Research, Westmead Hospital [Sydney], Département de médecine de l'enfant et de l'adolescent, Service de génétique [Tours], Hôpital Bretonneau-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Consultation de génétique, Clinique Saint-Jean Languedoc [Toulouse] (CSJL), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Genetique medicale, Centre hospitalier universitaire de Poitiers (CHU Poitiers), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Service de neurologie pédiatrique, Génétique Médicale, Centre hospitalier Bretagne Atlantique (Morbihan) (CHBA)-Hôpital Chubert, Service de rééducation fonctionnelle, and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau

Subjects
Male, Pathology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, medicine.disease_cause, Severity of Illness Index, GTP Phosphohydrolases, 0302 clinical medicine, Charcot-Marie-Tooth Disease, Genotype, Missense mutation, Child, Genes, Dominant, 0303 health sciences, Mutation, education.field_of_study, Middle Aged, 3. Good health, Phenotype, Child, Preschool, Connexin 32, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Hereditary motor and sensory neuropathy, Adult, Genetic Markers, medicine.medical_specialty, Adolescent, Mutation, Missense, Genes, Recessive, Biology, Mitochondrial Proteins, 03 medical and health sciences, Young Adult, Arts and Humanities (miscellaneous), Molecular genetics, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, Humans, education, 030304 developmental biology, Aged, [SDV.GEN]Life Sciences [q-bio]/Genetics, Myelin protein zero, Membrane Proteins, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, medicine.disease, Peripheral neuropathy, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Neurology (clinical), 030217 neurology & neurosurgery
Abstract

International audience; Background: Mutations in the gene encoding mito- fusin 2 (MFN2) cause Charcot-Marie-Tooth disease type 2 (CMT2), with heterogeneity concerning severity and associated clinical features. Objective: To describe MFN2 mutations and associ- ated phenotypes in patients with hereditary motor and sensory neuropathy (HMSN). Design: Direct sequencing of the MFN2 gene and clini- cal investigations of patients with MFN2 mutations. Setting: Molecular genetics laboratory of a university hospital and the LimogesNational Referral Center for Rare Peripheral Neuropathies. Patients: One hundred fifty index patients with HMSN and amedianmotor nerve conduction velocity of 25m/s or greater and without mutations in the genes encoding connexin 32 and myelin protein zero. Main Outcome Measures: Results of genetic analy- ses and phenotypic observations. Results: Twenty different missense mutations were identified in 20 index patients. Mutation frequency was 19 of 107 (17.8%) in patients with CMT2 and 1 of 43 (2.3%) in patients with a median motor nerve conduc- tion velocity less than 38 m/s. Four patients had proven de novo mutations, 8 families had autosomal dominant inheritance, and 3 had autosomal recessive inheritance. The remaining 5 patients were sporadic cases with het- erozygous mutations. Phenotypes varied from mild forms to early-onset severe forms. Additional features were encountered in 8 patients (32%). Six patients un- derwent sural nerve biopsy: electronic microscopy showed prominent mitochondrial abnormalities on longitudinal sections. Conclusions: MFN2 mutations are a frequent cause of CMT2, with variable severity and either dominant or recessive inheritance. MFN2 gene testing must be a first-line analysis in axonal HMSN irrespective of the mode of inheritance or the severity of the peripheral neuropathy.

Published
2009

11. Effects of knee and ankle muscle fatigue on postural control in the unipedal stance

Author

Yrieix Francois, Philippe Dupui, Jean Louis Jully, Thierry Paillard, Gerard Gonzalez, Riadh Bizid, Eric Margnes, Mouvement, Équilibre, Performance, Santé (MEPS), Université de Pau et des Pays de l'Adour (UPPA), Institut de Droit Européen des Droits de l'Homme - EA 3976 (IDEDH), Université de Montpellier (UM), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
Adult, Male, medicine.medical_specialty, Sports medicine, Physiology, Posture, Postural control, 03 medical and health sciences, 0302 clinical medicine, Voluntary contraction, Physiology (medical), Medicine, Humans, Orthopedics and Sports Medicine, Force platform, Knee, Foot pressure, Fatigue, ComputingMilieux_MISCELLANEOUS, Muscle fatigue, business.industry, Public Health, Environmental and Occupational Health, 030229 sport sciences, General Medicine, medicine.anatomical_structure, Isokinetic dynamometer, Physical therapy, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Ankle, business, 030217 neurology & neurosurgery
Abstract

The aim of this study was to compare the effects of acute muscle fatigue of the ankle and knee musculature on postural control by immediate measures after performing fatiguing tasks (POST condition). One group of subjects (n = 8) performed a fatiguing task by voluntary contractions of the triceps surae (group TRI) and the other (n = 9) performed a fatiguing task by voluntary contractions of the quadriceps femoris (group QUA). Each muscle group was exercised until the loss of maximal voluntary contraction torque reached 50% (isokinetic dynamometer). Posture was assessed by measuring the centre of foot pressure (COP) with a force platform during a test of unipedal quiet standing posture with eyes closed. Initially (in PRE condition), the mean COP velocity was not significantly different between group TRI and group QUA. In POST condition, the mean COP velocity increased more in group QUA than in group TRI. The postural control was more impaired by knee muscle fatigue than by ankle muscle fatigue.

Published
2009

12. Striking phenotypic variability in two familial cases of myosin storage myopathy with a MYH7 Leu1793pro mutation

Author

Pascale Richard, Marie-Christine Arne-Bes, Marie-Bernadette Delisle, Thierry Levade, Dominique Figarella-Branger, François Heitz, Jean-François Pellissier, Emmanuelle Uro-Coste, Service d'anatomie pathologique et histologie-cytologie [Rangueil], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Institut de médecine moléculaire de Rangueil (I2MR), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Service de pathologie et neuropathologie, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Unité Fonctionnelle de Cardiogénétique et Myogénétique Moléculaire et Cellulaire, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire de Biochimie [Purpan], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], Clinique Pasteur, Clinique Pasteur [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], and Simon, Marie Francoise

Subjects
DNA Mutational Analysis, Physiology, MESH: Myosin Heavy Chains, MESH: Genotype, 0302 clinical medicine, Myosin, MESH: Genetic Variation, MESH: DNA Mutational Analysis, MESH: Cardiac Myosins, Index case, Genetics (clinical), media_common, 0303 health sciences, Daughter, MESH: Muscle, Skeletal, MESH: Middle Aged, MESH: Genetic Predisposition to Disease, Heart, Middle Aged, MESH: Amino Acid Substitution, medicine.anatomical_structure, Phenotype, Neurology, MESH: Young Adult, Mutation (genetic algorithm), Muscle Hypotonia, Female, Cardiomyopathies, medicine.medical_specialty, Proximal muscle weakness, MESH: Mutation, MESH: Myocardium, Genotype, Proline, media_common.quotation_subject, Biology, MESH: Phenotype, 03 medical and health sciences, Young Adult, Muscular Diseases, Leucine, Internal medicine, medicine, Humans, Genetic Predisposition to Disease, Muscle, Skeletal, 030304 developmental biology, MESH: Proline, MESH: Humans, Myosin Heavy Chains, MESH: Muscle Hypotonia, Myocardium, MESH: Muscular Diseases, Skeletal muscle, Genetic Variation, MESH: Heart, Endocrinology, MESH: Leucine, Amino Acid Substitution, MESH: Cardiomyopathies, Pediatrics, Perinatology and Child Health, Mutation, MYH7, Neurology (clinical), Age of onset, Cardiac Myosins, MESH: Female, 030217 neurology & neurosurgery
Abstract

International audience; Myosin Storage Myopathies (MSM) have emerged as a new group of inherited myopathies with heterogenous clinical severity and age of onset. We have identified in a woman and her daughter, a pLeu1793Pro mutation in MYH7. This mutation has already been reported to be associated with MSM presenting as neonatal hypotony. Our index case complained of proximal muscle weakness at age 30. Her daughter presented at birth with a cardiomyopathy without any skeletal muscle involvement. This report underlines the clinical variability of MSM even with a given mutation or in a same family.

Published
2009

13. Effects of fatigue induced by neuromuscular electrical stimulation on postural control

Author

Yrieix Francois, Riadh Bizid, Gerard Gonzalez, Thierry Paillard, Philippe Dupui, Jean Louis Jully, Institut de Droit Européen des Droits de l'Homme - EA 3976 (IDEDH), Université de Montpellier (UM), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Mouvement, Équilibre, Performance, Santé (MEPS), and Université de Pau et des Pays de l'Adour (UPPA)

Subjects
Adult, Male, medicine.medical_specialty, Posture, Physical Therapy, Sports Therapy and Rehabilitation, Stimulation, Muscular Contractions, Postural control, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, medicine, Humans, Orthopedics and Sports Medicine, Spectral analysis, Force platform, Foot pressure, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Balance (ability), Analysis of Variance, Proprioception, business.industry, 030229 sport sciences, Electric Stimulation, Biomechanical Phenomena, Lower Extremity, Muscle Fatigue, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], business, 030217 neurology & neurosurgery, Muscle Contraction
Abstract

Summary The aim was to investigate the effects of fatigue induced by electrical stimulation superimposed onto voluntary muscular contractions on postural abilities and strategy (n = 8). Two groups of subjects performed a fatiguing of the triceps surae, the first by voluntary muscular contractions (group VOL) and the second (n = 8) by electrical stimulation superimposed onto voluntary muscle contractions (group VOL + ES). Balance was measured before and after the completion of both fatiguing tasks, by using a force platform which recorded centre of foot pressure (COP). Spatio-temporal COP parameters were used to evaluate the postural abilities, and a frequency analysis of the COP excursions (Fast Fourier Transform) was performed to estimate the postural strategy (low frequencies mostly account for visuo-vestibular regulation and high frequencies for proprioceptive regulation). In the post-fatigue condition, spatio-temporal COP parameters were not modified in either group. However, the frequency analysis showed that the high frequency band increased more in group VOL than in group VOL + ES. Calf-muscles fatigue did not impair postural control but it generated a change of the contribution of the proprioceptive information (myotatic loops) greater in group VOL than in group VOL + ES. Calf-muscles fatigue induced a modification of the postural strategy that may be exerted more strongly after voluntary muscular contractions than after electrical stimulation superimposed onto voluntary muscular contractions.

Published
2009

14. Effects of two types of neuromuscular electrical stimulation training on vertical jump performance

Author

Clement Hazard, Thierry Paillard, Philippe Dupui, Nicolas Bernard, Frédéric Noé, Mouvement, Équilibre, Performance, Santé (MEPS), Université de Pau et des Pays de l'Adour (UPPA), Littoral, Environnement, Télédétection, Géomatique (LETG - Brest), Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN), Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN), Université de Nantes (UN)-Université de Nantes (UN), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, and CHU Toulouse [Toulouse]

Subjects
Adult, Male, medicine.medical_specialty, Adolescent, [SDV]Life Sciences [q-bio], Movement, Physical Therapy, Sports Therapy and Rehabilitation, Stimulation, 03 medical and health sciences, Vertical jump, 0302 clinical medicine, Physical medicine and rehabilitation, Medicine, Humans, Orthopedics and Sports Medicine, Muscle Strength, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Electric stimulation, business.industry, 030229 sport sciences, General Medicine, Muscle endurance, Quadriceps femoris muscle, Electric Stimulation, 3. Good health, Lower Extremity, Muscle power, Muscle strength, Physical Endurance, business, Training program, 030217 neurology & neurosurgery, Psychomotor Performance
Abstract

This study examined the effects of different types of neuromuscular electrical stimulation (NMES) programs on vertical jump performance. Twenty seven healthy trained male students in sports-sciences were recruited and randomized into three groups. The control group (C group, n = 8) did not perform NMES training. Two other groups underwent 3 training sessions a week over 5 weeks on the quadriceps femoris muscle [F group (n = 9): stimulation with an 80 Hz current for 15 min for improving muscle strength; E group (n = 10): stimulation with a 25 Hz current for 60 min for improving muscle endurance]. The height of the vertical jump was measured before NMES training (test 1), one week (test 2) and five weeks (test 3) after the end of the programs. The results showed that the height of the vertical jump significantly increased in both the F and E groups between tests 1 and 2 (5 cm and 3 cm respectively). Results of test 3 showed that both groups preserved their gains. A NMES training program destined to improve muscle endurance does not interfere on vertical jump performance. It can even durably enhance it in the same way as a NMES training program destined to improve muscle strength. Thus, to improve muscle endurance without deteriorating muscle power, sportsmen can use electrical stimulation.

Published
2008

15. Slow wave sleep and recollection in recognition memory

Author

Agnès Daurat, Michel Tiberge, Patrice Terrier, Jean Foret, Cognition, Langues, Langage, Ergonomie (CLLE-LTC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Toulouse - Jean Jaurès (UT2J)-Centre National de la Recherche Scientifique (CNRS), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]

Subjects
Adult, Male, Polysomnography, Rapid eye movement sleep, Sleep, REM, [SHS.PSY]Humanities and Social Sciences/Psychology, Experimental and Cognitive Psychology, Context (language use), 050105 experimental psychology, Developmental psychology, 03 medical and health sciences, 0302 clinical medicine, Arts and Humanities (miscellaneous), Developmental and Educational Psychology, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, 0501 psychology and cognitive sciences, Slow-wave sleep, Recognition memory, Analysis of Variance, Recall, medicine.diagnostic_test, Memoria, 05 social sciences, Recognition, Psychology, Mental Recall, Wakefulness, Female, Sleep Stages, Psychology, 030217 neurology & neurosurgery, Cognitive psychology
Abstract

International audience; Recognition memory performance reflects two distinct memory processes: a conscious process of recollection, which allows remembering specific details of a previous event, and familiarity, which emerges in the absence of any conscious information about the context in which the event occurred. Slow wave sleep (SWS) and rapid eye movement (REM) sleep are differentially involved in the consolidation of different types of memory. The study assessed the effects of SWS and REM sleep on recollection, by means of the "remember"/"know" paradigm. Subjects studied three blocks of 12 words before a 3-h retention interval filled with SWS, REM sleep or wakefulness, placed between 3 a.m. and 6 a.m. Afterwards, recognition and recollection were tested. Recollection was higher after a retention interval rich in SWS than after a retention interval rich in REM sleep or filled with wakefulness. The results suggest that SWS facilitates the process of recollection in recognition memory.

Published
2007

16. Mos is required for MAP kinase activation and is involved in microtubule organization during meiotic maturation in the mouse

Author

Jacek Z. Kubiak, Michèle Weber, Marie-Hélène Verlhac, William H. Colledge, Martin J. Evans, Bernard Maro, Gérard Géraud, Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Physiology, Development and Neuroscience, University of Cambridge, Department of Mathematics [Berkeley], University of California [Berkeley], and University of California-University of California

Subjects
musculoskeletal diseases, endocrine system, Proto-Oncogene Proteins c-mos, [SDV]Life Sciences [q-bio], Maturation-Promoting Factor, Maturation promoting factor, Spindle Apparatus, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Protein Serine-Threonine Kinases, Microtubules, Mice, 03 medical and health sciences, 0302 clinical medicine, Meiosis, Proto-Oncogene Proteins, medicine, Animals, Molecular Biology, reproductive and urinary physiology, 030304 developmental biology, 0303 health sciences, MAP kinase kinase kinase, biology, urogenital system, Oocyte, Molecular biology, Chromatin, Cell biology, Spindle apparatus, Enzyme Activation, Proto-Oncogene Proteins c-raf, medicine.anatomical_structure, Mitogen-activated protein kinase, Calcium-Calmodulin-Dependent Protein Kinases, embryonic structures, Oocytes, biology.protein, Female, 030217 neurology & neurosurgery, Developmental Biology
Abstract

International audience; Mos is normally expressed during oocyte meiotic maturation in vertebrates. However, apart from its cytostatic factor (CSF) activity, its precise role during mouse meiosis is still unknown. First, we analyzed its role as a MAP kinase kinase kinase. Mos is synthesized concomitantly with the activation of MAP kinase in mouse oocytes. Moreover, MAP kinase is not activated during meiosis in oocytes from mos -/- mice. This result implies that Mos is necessary for MAP kinase activation in mouse oocytes. Raf-1, another MAP kinase kinase kinase, is already present in immature oocytes, but does not seem to be active when MAP kinase is activated. Moreover, the absence of MAP kinase activation in mos -/- oocytes demonstrates that Raf-1 cannot compensate for the lack of Mos. These results suggest that Raf-1 is not involved in MAP kinase activation. Second, we analyzed the organization of the microtubules and chromosomes in oocytes from mos -/- mice. We observed that during the transition between two meiotic M-phases, the microtubules and chromosomes evolve towards an interphase-like state in mos -/- oocytes, while in the control mos +/- oocytes they remain in an M-phase configuration, as in the wild type. Moreover, after spontaneous activation, the majority of mos -/- oocytes are arrested for at least 10 hours in a third meiotic M-phase where they exhibit monopolar half-spindles. These observations present the first evidence, in intact oocytes, of a role for the Mos/.../MAP kinase cascade in the control of microtubule and chromatin organization during meiosis.

Published
1996

17. Forskolin blocks the apical expression of dipeptidyl peptidase IV in Caco-2 cells and induces its retention in lamp-1-containing vesicles

Author

Christian Cibert, Patrice Codogno, Catherine Sapin, Gérard Géraud, Martine Garcia, Germain Trugnan, Laurent Baricault, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Archéologies, Cultures et Sociétés (ACS), Ministère de la Culture et de la Communication (MCC)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Trafic Membranaire et Signalisation Dans les Cellules Epitheliales, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Recherche Saint-Antoine (UMRS893), Centre d’Ecologie Fonctionnelle et Evolutive ( CEFE ), Université Paul-Valéry - Montpellier 3 ( UM3 ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -École pratique des hautes études ( EPHE ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Archéologies, Cultures et Sociétés ( ACS ), Ministère de la Culture et de la Communication ( MCC ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paul Sabatier - Toulouse 3 ( UPS ) -CHU Toulouse [Toulouse]-Hôpital de Rangueil, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de Recherche Saint-Antoine ( CR Saint-Antoine ), Université Pierre et Marie Curie - Paris 6 ( UPMC ), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)

Subjects
Brush border, Dipeptidyl Peptidase 4, Blotting, Western, Fluorescent Antibody Technique, In Vitro Techniques, Biology, Dipeptidyl peptidase, Adenylyl cyclase, 03 medical and health sciences, chemistry.chemical_compound, symbols.namesake, Antigens, CD, [ CHIM.ORGA ] Chemical Sciences/Organic chemistry, Image Processing, Computer-Assisted, Tumor Cells, Cultured, Humans, Dipeptidyl-Peptidases and Tripeptidyl-Peptidases, 030304 developmental biology, 0303 health sciences, Membrane Glycoproteins, Forskolin, Microvilli, 030306 microbiology, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Colforsin, Microfilament Proteins, Lysosome-Associated Membrane Glycoproteins, Membrane Proteins, Cell Differentiation, Cell Biology, Golgi apparatus, Apical membrane, Precipitin Tests, Cell Compartmentation, Cell biology, Intestines, chemistry, Biochemistry, Cell culture, symbols, Carrier Proteins, Lysosomes, Intracellular
Abstract

International audience; In a previous work, we showed that the differentiation-dependent expression of dipeptidyl peptidase IV (DPP IV) in Caco-2 cells, a human colon adenocarcinoma cell line, was controlled at the mRNA level (D. Darmoul et al. J. Biol. Chem., 1992, 267, 4824-4833). Whether post-translational events may contribute to the final control of DPP IV cell surface expression was explored here by studying the potential effect of forskolin (FK), a drug known to permanently stimulates adenylyl cyclase and to strongly perturbs glucose metabolism in fully differentiated Caco-2 cells. FK treatment reduces by about 50% the amount of active DPP IV present at the brush border membrane, whereas the total amount of active DPP IV remains unchanged. Biosynthesis and maturation of DPP IV were measured using [35S]methionine labeling and were shown to be essentially unaffected by FK treatment. Pulse-chase experiments demonstrate that up to 50% of the neosynthesized DPP IV do not appear at the apical membrane after FK treatment. To get further insight into this phenomenon, we have used confocal laser scanning microscopy. We demonstrate that the blockade of DPP IV transport is associated with the accumulation of this protein in intracellular vesicles. Double-staining experiments demonstrate that these vesicles are not labeled with a monoclonal antibody directed against the Golgi apparatus but display a strong staining with a polyclonal antibody raised against lamp-1, a lysosomal membrane protein. Using a newly developed image analysis procedure, we have been able to quantitate the relative distribution of lamp-1 and DPP IV labels in both control and forskolin-treated cells. We show that the overlap of the two labels dramatically increases in FK-treated Caco-2 cells. These results suggest that, beside the transcriptional level, post-translational events may be involved in the final control of the apical expression of a differentiation-dependent hydrolase.

Published
1993

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