1. A Novel Pathway for Sesquiterpene Biosynthesis fromZ,Z-Farnesyl Pyrophosphate in the Wild TomatoSolanum habrochaites
- Author
-
Christophe Sallaud, Françoise Jabès, Sandrine Onillon, Denis Rontein, Gaëtan Herbette, Mathilde Causse, Nathalie Leonhardt, Alain Tissier, Camille Escoffier, P. Duffe, Cécile Giacalone, Samuel Thoraval, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Université Paul Cézanne - Aix-Marseille 3, Université de la Méditerranée - Aix-Marseille 2, Laboratoire des Echanges Membranaires et Signalisation (LEMS), Université de la Méditerranée - Aix-Marseille 2-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), and ANR05PRIB02101 more...
- Subjects
0106 biological sciences ,DNA, Plant ,Molecular Sequence Data ,Farnesyl pyrophosphate ,Plant Science ,Genes, Plant ,Solanum ,Sesquiterpene ,01 natural sciences ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,chemistry.chemical_compound ,Polyisoprenyl Phosphates ,Biosynthesis ,Tobacco ,Amino Acid Sequence ,Cloning, Molecular ,Plastid ,Phylogeny ,Research Articles ,Plant Proteins ,030304 developmental biology ,Expressed Sequence Tags ,0303 health sciences ,Alkyl and Aryl Transferases ,ATP synthase ,biology ,Chromosome Mapping ,food and beverages ,Geranyltranstransferase ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Recombinant Proteins ,Terpenoid ,Chloroplast ,Biochemistry ,chemistry ,biology.protein ,Nicotiana sylvestris ,Sequence Alignment ,Sesquiterpenes ,010606 plant biology & botany - Abstract
In the wild tomato Solanum habrochaites, the Sst2 locus on chromosome 8 is responsible for the biosynthesis of several class II sesquiterpene olefins by glandular trichomes. Analysis of a trichome-specific EST collection from S. habrochaites revealed two candidate genes for the synthesis of Sst2-associated sesquiterpenes. zFPS encodes a protein with homology to Z-isoprenyl pyrophosphate synthases and SBS (for Santalene and Bergamotene Synthase) encodes a terpene synthase with homology to kaurene synthases. Both genes were found to cosegregate with the Sst2 locus. Recombinant zFPS protein catalyzed the synthesis of Z,Z-FPP from isopentenylpyrophosphate (IPP) and dimethylallylpyrophosphate (DMAPP), while coincubation of zFPS and SBS with the same substrates yielded a mixture of olefins identical to the Sst2-associated sesquiterpenes, including (+)-α-santalene, (+)-endo-β-bergamotene, and (−)-endo-α-bergamotene. In addition, headspace analysis of tobacco (Nicotiana sylvestris) plants expressing zFPS and SBS in glandular trichomes afforded the same mix of sesquiterpenes. Each of these proteins contains a putative plastid targeting sequence that mediates transport of a fused green fluorescent protein to the chloroplasts, suggesting that the biosynthesis of these sesquiterpenes uses IPP and DMAPP from the plastidic DXP pathway. These results provide novel insights into sesquiterpene biosynthesis and have general implications concerning sesquiterpene engineering in plants. more...
- Published
- 2009
- Full Text
- View/download PDF