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Start Over Descriptor "Phialophora mustea" Topic 03 medical and health sciences
3 results on '"Phialophora mustea"'

1. Unravelling the Role of Melanin in Cd and Zn Tolerance and Accumulation of Three Dark Septate Endophytic Species

Author

Gábor M. Kovács, Asfaw Zegeye, Corinne Leyval, Charlotte Berthelot, Dalia A. Gaber, Michel Chalot, Philipp Franken, Damien Blaudez, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL), Assiut University, Leibniz Institute of Vegetable and Ornamental Crops (IGZ), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Faculté des Sciences et Technologies [Université de Lorraine] (FST ), Université de Lorraine (UL), Fachhochschule Erfurt [Erfurt], Plant Protection Institute [Budapest] (ATK NOVI), Centre for Agricultural Research [Budapest] (ATK), Hungarian Academy of Sciences (MTA)-Hungarian Academy of Sciences (MTA), Department of Plant Anatomy, Institute of Biology [Budapest], Faculty of Sciences [Budapest], Eötvös Loránd University (ELTE)-Eötvös Loránd University (ELTE)-Faculty of Sciences [Budapest], Eötvös Loránd University (ELTE)-Eötvös Loránd University (ELTE), This work was supported by the LORVER project funded by the Région Grand-Est and the EuropeanRegional Development Fund (ERDF), by the Groupement d’Intérêt Scientifique sur les Friches Industrielles (GISFI),by the French Environment and Energy Management Agency [PROLIPHYT ADEME-1172C0053] and by theAgence Nationale de la Recherche (ANR) under the Blanc International ANR-10-INTB-1703-01-BIOFILTREE project.The support by the ELTE Institutional Excellence Program supported by the National Research, Development andInnovation Oce (NKFIH-1157-8/2019-DT) is also acknowledged (GMK), ANR-10-INTB-1703,BIOFILTREE,Filtration biologique pour la réduction des éléments traces dans la biomasse des arbres(2010), Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Observatoire Terre et Environnement de Lorraine (OTELo), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UFC (UMR 6249) (LCE), Eötvös Loránd University (ELTE), and Hungarian Academy of Sciences (MTA)

Subjects
Microbiology (medical), Hypha, [SDV]Life Sciences [q-bio], Phialophora mustea, Mutant, Microbiology, Article, Melanin, Cadophora sp, 03 medical and health sciences, chemistry.chemical_compound, Melanin synthesis, Virology, Leptodontidium sp, sulcotrione, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 030304 developmental biology, tricyclazole, 0303 health sciences, Strain (chemistry), integumentary system, kojic acid, 030306 microbiology, chemistry, lcsh:Biology (General), [SDE]Environmental Sciences, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Kojic acid, Leptodontidium, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

Dark septate endophytes (DSEs) are often trace element (TE)-tolerant fungi and are abundant in TE-polluted environments. The production of melanin, a black polymer found in cell walls, was hypothesized by several authors to play a role in the TE tolerance of DSEs. To test this hypothesis, we established a series of experiments using albino strains and melanin inhibitors and examined the responses to Cd and Zn. Six DSEs belonging to genera Cadophora sp., Leptodontidium sp. and Phialophora mustea, were evaluated. The strains mainly produced 1,8-dihydroxynaphthalene (DHN) melanin whereas 3,4-dihydroxyphenylalanin melanin was also synthetized. Cd and Zn decreased melanin synthesis in most of the strains. A reduction in melanin concentration in hyphae through the use of tricyclazole, an inhibitor of DHN-melanin synthesis, did not reduce the tolerance of the strains to Cd and Zn. Similarly, albino mutants of Leptodontidium sp. were not more sensitive to Cd and Zn than the WT strain. Moreover, tricyclazole-treated colonies accumulated less Cd but more Zn compared to untreated colonies. The Cd and Zn contents of Leptodontidium albino strains were variable and similar to that of the WT. The results suggest that melanin production is not an important functional trait that contributes to Cd and Zn tolerance, but might contribute to Cd accumulation.

Published
2020
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2. Interactions between dark septate endophytes, ectomycorrhizal fungi and root pathogens in vitro

Author

Corinne Leyval, Charlotte Berthelot, Damien Blaudez, Michel Chalot, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Phytophthora citricola, ectomycorrhizal fungi, Microorganism, root pathogenic fungi, Phialophora mustea, [SDV]Life Sciences [q-bio], Heterobasidion annosum, Biological pest control, Phialocephala fortinii, Context (language use), Fungus, Dark septate endophyte, Plant Roots, 01 natural sciences, Microbiology, 03 medical and health sciences, Cadophora sp, Mycorrhizae, Phialophora, Botany, Endophytes, Genetics, Symbiosis, Molecular Biology, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Leptodontidium sp, biology, Fungi, food and beverages, 15. Life on land, biology.organism_classification, Phenotype, 010606 plant biology & botany
Abstract

Dark septate endophytes (DSEs) are widely distributed worldwide and can promote plant growth. Therefore, they are considered potentially important plant allies, especially in stressful environments. Previous studies have reported that DSEs cohabit roots with other microorganisms such as ectomycorrhizal (ECM), endophytic and pathogenic fungi/oomycetes. However, interactions between different DSE species have not yet been reported, and studies on the interactions between DSEs and other fungi are scarce. Using a simple and reproducible pairwise growth assay in vitro, we studied the synergistic/antagonistic interactions between eight DSEs, two ECM fungi and three root pathogens. Most of the DSE/DSE outcomes were neutral. Interestingly, we identified several DSE strains acting in synergy with other strains, as well as strains that could potentially act as biocontrol agents. Notably, three metal-tolerant DSE strains, namely, Cadophora sp., Leptodontidium sp. and Phialophora mustea, could decrease the growth of the root phytopathogens Pythium intermedium, Phytophthora citricola and Heterobasidion annosum. The present data are discussed in the general context of the use of fungal consortia as inocula in the tree-based phytomanagement of marginal lands.

Published
2019
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3. Melanization and ageing are not drawbacks for successful agro-transformation of dark septate endophytes

Author

Corinne Leyval, Yoann Perrin, Damien Blaudez, Charlotte Berthelot, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), and Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Hypha, Phialophora mustea, Cladosporium cladosporioides, Plant use of endophytic fungi in defense, Melanin, 03 medical and health sciences, Transformation, Genetic, Ascomycota, Botany, Genetics, Endophytes, Ecology, Evolution, Behavior and Systematics, Mycelium, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, 2. Zero hunger, Melanins, endophytic fungi, biology, fungi, 15. Life on land, biology.organism_classification, Leptodontidium, melanin, Transformation (genetics), Thiazoles, 030104 developmental biology, Infectious Diseases, Agrobacterium tumefaciens, Cadophora, Cladosporium, Cadophora malorum, Copper
Abstract

International audience; Dark septate endophytes (DSEs) are melanin-enriched ascomycetous fungi that are abundant in stressed environments. However, little is known about their physiology and metabolism, and DSE genes have not been functionally characterized yet. Therefore developing molecular genetic tools to investigate the biological function of genes of interest in DSEs is of major significance. We investigated Agrobacterium tumefaciens-mediated transformation (ATMT) efficiency in eight DSE strains belonging to Cadophora sp., Cadophora malorum, Leptodontidium sp., Phialophora mustea, and Cladosporium cladosporioides. ATMT efficiency was DSE-dependent and ranged from 0.6% to 125 %. The transformants were mitotically stable and expressed the gfp marker. The patterns of transformant appearance as a function of time were diverse and also strain-dependent. We further focused on the effect of mycelium ageing on ATMT efficiency. Leptodontidium sp. Me07, Leptodontidium sp. Pr30, and C. cladosporioides CBS 101367 were significantly more transformed using fifteen-day-old mycelium (44.5, 6.9, and 1.1%, respectively) as compared to two-day-old mycelium (121, 28.7, and 25.1, respectively), whereas P. mustea Pr29 was more transformed using young mycelium (21.5% compared to 5.3% for the old mycelium). Finally, we focused on the effect of melanin content on ATMT efficiency. Melanin content in mycelium ranged from 0.9 to 3.2 mg per gram of dry weight. Tricyclazole, an inhibitor of melanin biosynthesis, negatively modulated melanin content, while copper, an inducer of melanin biosynthesis, positively modulated them. However there was no correlation between hyphal melanin content and ATMT efficiency.

Published
2017
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