1. Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms
- Author
-
Victor Kuete, Minxia Fan, Mingquan Guo, Satyajit D. Sarker, and Armel Jackson Seukep
- Subjects
solvent fractions ,Ethyl acetate ,RV ,Plant Science ,01 natural sciences ,Article ,Bacterial cell structure ,03 medical and health sciences ,chemistry.chemical_compound ,Minimum inhibitory concentration ,0302 clinical medicine ,lcsh:Botany ,modes of action ,Plukenetia huayllabambana ,GC–MS ,Ecology, Evolution, Behavior and Systematics ,Chromatography ,Ecology ,010405 organic chemistry ,Phytosterol ,Broth microdilution ,lcsh:QK1-989 ,0104 chemical sciences ,Solvent ,antibacterial ,chemistry ,030220 oncology & carcinogenesis ,Gas chromatography–mass spectrometry ,Antibacterial activity - Abstract
Plukenetia huayllabambana is an edible plant traditionally used to cure wounds and various infections. The present work assessed, for the first time, the antibacterial efficacy of solvent fractions from P. huayllabambana fruits. The crude methanol extract was obtained applying ultrasound-assisted extraction, followed by partitioning through successive depletion among solvents of increasing polarity to yield fractions (n-hexane, dichloromethane, ethyl acetate, and n-butanol). The minimal inhibitory concentration (MIC) was determined following antibacterial testing, using the broth microdilution technique against a panel of drug-resistant Gram-negative and Gram-positive bacteria. Possible modes of action of the most active fraction were also investigated. Gas chromatography&ndash, mass spectrometry (GC&ndash, MS) was used to identify phytocompounds that may account for the recorded activities. Methanol, n-hexane (PH-n-Hex), and ethyl acetate extracts inhibited 100% of studied bacteria, with the recorded MIC ranging from 0.125&ndash, 1 mg/mL. PH-n-Hex appeared as the most active partition, exerting a bacteriostatic effect. PH-n-Hex probably acts by interfering with bacterial biofilm formation, proton pumps, and bacterial cell membrane integrity. The GC&ndash, MS analysis of PH-n-Hex led to the identification of 11 potentially bioactive components, including fatty acids, phytosterol, and diterpene alcohol as major ones. P. huayllabambana can be considered as a plant of pharmacological value&mdash, a source of potent anti-infective drug entities.
- Published
- 2020