1. A limited role of NKCC1 in telencephalic glutamatergic neurons for developing hippocampal network dynamics and behavior
- Author
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Christian A. Hübner, Chuanqiang Zhang, Anja Urbach, Knut Holthoff, Stefan J. Kiebel, Christiane Frahm, Vahid Rahmati, Madlen Guenther, Knut Kirmse, Tanja Herrmann, Ricardo Melo Neves, Otto W. Witte, Robin Hinsch, Tom Flossmann, Dirk Isbrandt, Jürgen Graf, and Stephan Lawrence Marguet
- Subjects
0301 basic medicine ,hippocampus ,Glutamic Acid ,Hippocampus ,Biology ,Hippocampal formation ,03 medical and health sciences ,Glutamatergic ,Mice ,GABA ,0302 clinical medicine ,In vivo ,medicine ,NKCC1 ,Solute Carrier Family 12, Member 2 ,Animals ,physiology [Solute Carrier Family 12, Member 2] ,metabolism [gamma-Aminobutyric Acid] ,development ,gamma-Aminobutyric Acid ,Visual Cortex ,Neurons ,gaba ,Multidisciplinary ,nkcc1 ,metabolism [Glutamic Acid] ,Transporter ,metabolism [Synapses] ,Biological Sciences ,growth & development [Hippocampus] ,Electrophysiology ,in vivo ,030104 developmental biology ,Visual cortex ,medicine.anatomical_structure ,Animals, Newborn ,metabolism [Neurons] ,Synapses ,Excitatory postsynaptic potential ,ddc:500 ,physiology [Visual Cortex] ,Nerve Net ,Neuroscience ,030217 neurology & neurosurgery - Abstract
NKCC1 is the primary transporter mediating chloride uptake in immature principal neurons, but its role in the development of in vivo network dynamics and cognitive abilities remains unknown. Here, we address the function of NKCC1 in developing mice using electrophysiological, optical, and behavioral approaches. We report that NKCC1 deletion from telencephalic glutamatergic neurons decreases in vitro excitatory actions of gamma-aminobutyric acid (GABA) and impairs neuronal synchrony in neonatal hippocampal brain slices. In vivo, it has a minor impact on correlated spontaneous activity in the hippocampus and does not affect network activity in the intact visual cortex. Moreover, long-term effects of the developmental NKCC1 deletion on synaptic maturation, network dynamics, and behavioral performance are subtle. Our data reveal a neural network function of NKCC1 in hippocampal glutamatergic neurons in vivo, but challenge the hypothesis that NKCC1 is essential for major aspects of hippocampal development.
- Published
- 2021
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