1. Chemical Characterization and Antioxidant, Antimicrobial, and Anti-Inflammatory Activities of South Brazilian Organic Propolis
- Author
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Severino Matias de Alencar, Pedro Luiz Rosalen, Carina Denny, Marcelo Franchin, Ana Paula Tiveron, Risia Cristina Coelho Lacerda, Masaharu Ikegaki, Bruno Bueno-Silva, and Bruna Benso
- Subjects
0301 basic medicine ,Chemical Radicals ,Antioxidant ,medicine.medical_treatment ,PRÓPOLIS ,Anti-Inflammatory Agents ,lcsh:Medicine ,Pathology and Laboratory Medicine ,Biochemistry ,Physical Chemistry ,Antioxidants ,Mice ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Food science ,lcsh:Science ,Liquid Chromatography ,chemistry.chemical_classification ,Multidisciplinary ,Antimicrobials ,Chromatographic Techniques ,NF-kappa B ,Drugs ,Pseudomonas Aeruginosa ,Free Radical Scavengers ,Antimicrobial ,Anti-Bacterial Agents ,Bacterial Pathogens ,Chemistry ,Medical Microbiology ,030220 oncology & carcinogenesis ,Physical Sciences ,Pathogens ,Cellular Types ,Research Article ,Free Radicals ,medicine.drug_class ,Immune Cells ,Immunology ,Biology ,Research and Analysis Methods ,Microbiology ,Propolis ,Anti-inflammatory ,03 medical and health sciences ,Minimum inhibitory concentration ,Phenols ,Streptococcal Infections ,Streptococcus Mutans ,Microbial Control ,Pseudomonas ,medicine ,Animals ,Humans ,Pseudomonas Infections ,Microbial Pathogens ,Pharmacology ,Reactive oxygen species ,Blood Cells ,Bacteria ,Tumor Necrosis Factor-alpha ,Macrophages ,lcsh:R ,Organisms ,Chemical Compounds ,Streptococcus ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Streptococcus mutans ,High Performance Liquid Chromatography ,RAW 264.7 Cells ,030104 developmental biology ,Streptococcus oralis ,chemistry ,lcsh:Q - Abstract
South Brazilian organic propolis (OP), which has never been studied before, was assessed and its chemical composition, scavenging potential of reactive oxygen species, antimicrobial and anti-inflammatory activities are herein presented. Based on the chemical profile obtained using HPLC, OP was grouped into seven variants (OP1–OP7) and all of them exhibited high scavenging activity, mainly against superoxide and hypochlorous acid species. OP1, OP2, and OP3 had the smallest minimal inhibitory concentration (MIC) against Gram-positive bacteria Streptococcus mutans, Streptococcus oralis, and Streptococcus aureus (12.5–100 μg/mL). OP1, OP2, OP3, and OP4 were more effective against Pseudomonas aeruginosa (Gram-negative), with MIC values ranging from 100 to 200 μg/mL. OP6 showed anti-inflammatory activity by decreasing NF-kB activation and TNF-α release in RAW 264.7 macrophages, and expressing the NF-κB-luciferase reporter stable gene. Therefore, south Brazilian OP can be considered an excellent source of bioactive compounds with great potential of application in the pharmaceutical and food industry.
- Published
- 2016
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