75 results on '"Thais Marchini de Oliveira"'
Search Results
2. Simplified occlusal replica adapted technique with glass ionomer cement for molar-incisor hypomineralization–affected molars
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Thais Marchini de Oliveira, Fernanda Lyrio Mendonça, Thiago Cruvinel, Daniela Rios, Isabella Claro Grizzo, C. C. L. Leone, and Maria Fidela de Lima Navarro
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Molar ,Enamel paint ,business.industry ,medicine.medical_treatment ,Glass ionomer cement ,Dentistry ,030206 dentistry ,Molar Incisor Hypomineralization ,stomatognathic diseases ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Dental clinic ,visual_art ,visual_art.visual_art_medium ,Medicine ,business ,General Dentistry ,Practical implications ,Dental restoration ,Month follow up - Abstract
Background and Overview Molar-incisor hypomineralization (MIH) is a qualitative developmental defect of enamel, which has been a daily concern in clinical practice owing to its difficult treatment. Case Description An 11-year-old girl was admitted to the pediatric dental clinic with a symptom of severe hypersensitivity in the permanent molars owing to MIH. The treatment consisted of a simplified occlusal replica adapted technique with conventional glass ionomer cement in the MIH-affected molars, which involved laboratory and clinical stages. The patient was reexamined every 3 months, and after 18 months there was no additional tooth structure loss, restoration failure, or pain. This technique may be considered a feasible alternative to restore hypomineralized teeth owing to its reduced procedure time and good performance in the follow-up period of 18 months. Conclusions and Practical Implications The technique successfully restored the function and shape of the affected teeth, decreasing the sensitivity by means of covering the demarcated MIH opacities.
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- 2020
3. Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study
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Thais Marchini de Oliveira, Rodrigo Cardoso de Oliveira, Maria Aparecida de Andrade Moreira Machado, Carlos Ferreira dos Santos, Nathália Martins Lopes, Luciana Lourenço Ribeiro Vitor, Natalino Lourenço Neto, Camila Oliveira Rodini, Thiago José Dionísio, and Mariel Tavares Oliveira Prado Bergamo
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Lysis ,Cell Survival ,Dermatology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Protein biosynthesis ,Humans ,Multiplex ,Crystal violet ,Viability assay ,Angiogenic Proteins ,Low-Level Light Therapy ,Tooth, Deciduous ,Child ,Cells, Cultured ,Dental Pulp ,Cell Proliferation ,CÉLULAS-TRONCO ,biology ,Stem Cells ,030206 dentistry ,Molecular biology ,chemistry ,Child, Preschool ,biology.protein ,Surgery ,Lasers, Semiconductor ,Stem cell ,Biomarkers ,Platelet-derived growth factor receptor - Abstract
This study evaluated the viability, proliferation, and protein expression after photobiomodulation (PBM) of stem cell from human exfoliated deciduous teeth (SHED). The groups were the following: G1 (2.5 J/cm2), G2 (3.7 J/cm2), and control (not irradiated). According to the groups, cells were irradiated with InGaAlP diode laser at 660 nm wavelength, continuous mode, and single time application. After 6 h, 12 h, and 24 h from irradiation, the cell viability and proliferation, and the protein expression were analyzed by MTT, crystal violet, and ELISA multiplex assay, respectively. Twenty-four hours after PBM, SHED showed better proliferation. Over time in the supernatant, all groups had an increase at the levels of VEGF-C, VEGF-A, and PLGF. In the lysate, the control and G2 exhibited a decrease of the VEGF-A, PECAM-1, and PLGF expression, while control and G3 decreased VEGF-C, VEGF-A, and PDGF expression. The dosimetries of 2.5 J/cm2 and 3.7 J/cm2 maintained viability, improved proliferation, and synthesis of the angiogenic proteins in the supernatant in the studied periods on SHED.
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- 2020
4. Stereophotogrammetry to evaluate young adults with and without cleft lip and palate after orthodontic and restorative treatment
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Thais Marchini de Oliveira, Simone Soares, Maria Giulia Rezende Pucciarelli, and Guilherme Hideki de Lima Toyoshima
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Molar ,Cleft Lip ,medicine.medical_treatment ,Calcium Sulfate ,Prosthesis ,Young Adult ,03 medical and health sciences ,Dental Arch ,0302 clinical medicine ,Maxilla ,medicine ,Humans ,In patient ,Young adult ,Retrospective Studies ,Orthodontics ,business.industry ,Significant difference ,030206 dentistry ,medicine.disease ,Cleft Palate ,Restorative treatment ,stomatognathic diseases ,Dental arch ,PRÓTESES E IMPLANTES ,medicine.anatomical_structure ,Photogrammetry ,Agenesis ,Oral Surgery ,business - Abstract
Statement of problem Patients with cleft lip and palate generally present with lateral incisor agenesis, which may be treated with an implant-supported prosthesis. However, whether implants can stabilize the dental arches in patients with cleft lip and palate is unclear. Purpose The purpose of this retrospective clinical study was to analyze the stability of the maxillary dental arch after orthodontic treatment and oral rehabilitation in the cleft area with an implant-supported prosthesis or a fixed partial denture. Material and methods Fifty-five participants, 20 with unilateral cleft lip and palate rehabilitated with implants (CLPI), 15 with unilateral cleft lip and palate rehabilitated with fixed partial dentures (CLPFP), and 20 in the noncleft group (NCLP) and their gypsum casts (N=110) were digitized and evaluated through 3D stereophotogrammetry. Measurements were made on casts obtained immediately after the orthodontic treatment (T1); for the cleft lip and palate group, casts were made 1 year after implant-supported restoration placement (T2), and for the noncleft group, 1 year after the conclusion of the orthodontic treatment (T2). The dimensions of the dental arches were measured digitally. Formula Δ=T2-T1 evaluated the stability of dental arches for intercanine distances, intermolar distances, arch length, palate surface, and volume (3D). Stability (Δ) was compared through 1-way ANOVA in all groups (α=.05). Results A statistically significant difference was found in the stability of the CLPI and CLPFP groups for intercanine measurement (P=.002). For the intermolar measurement, a statistically significant difference was detected between the CLPFP and NCLP groups (P=.002). From the 3D measurements, the stability was similar in all groups. Conclusions In patients with clefts, a fixed partial denture may provide better stability of the orthodontic outcomes than an implant-supported prosthesis. However, greater instability occurred at the molar area.
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- 2022
5. Analysis of Dental Arch in Children With Oral Cleft Before and After the Primary Surgeries
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Bianca Zeponi Fernandes Mello, Thais Marchini de Oliveira, Marcio De Menezes, Cleide Felício de Carvalho Carrara, Eloá Cristina Passucci Ambrosio, Paula Karine Jorge, Simone Soares, Maria Aparecida de Andrade Moreira Machado, and Fabrício Pinelli Valarelli
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Wilcoxon signed-rank test ,Cleft Lip ,medicine.medical_treatment ,03 medical and health sciences ,Dental Arch ,Imaging, Three-Dimensional ,0302 clinical medicine ,FISSURA LÁBIOPALATINA ,Maxilla ,Humans ,Medicine ,Child ,030223 otorhinolaryngology ,Orthodontics ,Oral cleft ,business.industry ,030206 dentistry ,General Medicine ,Unilateral complete cleft lip ,Cleft Palate ,stomatognathic diseases ,Dental arch ,Treatment Outcome ,medicine.anatomical_structure ,Palatoplasty ,Otorhinolaryngology ,Surgery ,Analysis of variance ,Cheiloplasty ,business - Abstract
This study aimed to evaluate longitudinally the alteration of the dental arch dimensions of children with different oral cleft types, before and after the primary surgeries. Three-dimensional images of the maxillary dental casts of children with unilateral complete cleft lip (G1), unilateral complete cleft lip and palate (G2), and cleft palate (G3). The children were evaluated at pre-cheiloplasty (T1), pre-palatoplasty (T2), and 1 year after palatoplasty (T3). The measurements obtained: intercanine (C-C') and intertuberosity (T-T') distances, anterior (I-CC') and total (I-TT') arch lengths. To analyze the intraexaminer error, paired t-test was applied and Dahlbergh formula. The intragroups comparisons were applied: paired t-test, ANOVA followed by Tukey, Wilcoxon test, and Kruskal-Wallis test followed by Dunn test. The intergroup comparisons were performed by independent t-test and Mann-Whitney test. In G1, the C-C', T-T', and the I-TT' distances revealed a significant increase of the maxilla. In G2, the C-C' distance statistically decreased from T1 to T3, T-T' distance showed statistical increase from T1 to T3. The I-TT' length increased with statistically significant differences between T1 and T3, T2 and T3. In G3, the C-C', T-T', and I-TT' distance increased was statistically significant. The longitudinal evaluation of the changes occurred in the dental arches with different oral cleft types showed that cheiloplasty and palatoplasty caused the most alterations in the development of the maxillary dimensions of children with complete cleft lip and palate.
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- 2019
6. Do Cleft Lip and Palate Types Affect Health-Related Quality of Life of Adolescents?
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Simone Soares, Ana Lúcia Pompéia Fraga de Almeida, Daniela Rios, Thainara Alves Crepaldi, Cleide Felício de Carvalho Carrara, Maria Aparecida de Andrade Moreira Machado, Thiago Cruvinel, Thais Marchini de Oliveira, and Luciana Lourenço Ribeiro Vitor
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Male ,Adolescent ,Cleft Lip ,Emotions ,Dentistry ,Affect (psychology) ,ADOLESCENTES ,03 medical and health sciences ,0302 clinical medicine ,Quality of life ,Surveys and Questionnaires ,Humans ,Medicine ,030223 otorhinolaryngology ,Health related quality of life ,Oral cleft ,business.industry ,Confounding ,030206 dentistry ,General Medicine ,Mental health ,Cleft Palate ,Mental Health ,Otorhinolaryngology ,Bilateral cleft lip ,Quality of Life ,Mann–Whitney U test ,Female ,Surgery ,business - Abstract
The adolescent with oral cleft must deal with the process of body changing and the psychological implications caused by morphological, functional, and esthetic impairments of the cleft itself. This study aimed to evaluate whether the cleft lip and palate types affects health-related quality of life of adolescents through Short-form Health Survey (SF-36) questionnaire. Fifty-seven adolescents (29 males and 28 females; mean age 15 years and 11 months ± 1 year and 2 months) were divided into 2 groups: Group 1-single cleft-unilateral or bilateral CL or CP; Group 2-complex cleft-unilateral or bilateral cleft lip and palate. The participants filled in SF-36 questionnaire to verify their functional, physical, and mental well-being profile. Spearman test assessed SF-36 scores correlation with age. Mann-Whitney U test verified the differences between genders and cleft types. Linear regression models were used to analyze confounding factors (age and gender). P was set at0.05. The different SF-36 domains weakly correlated with age, ranging from -0.07 (P = 0.60) for the social aspects and 0.31 (P = 0.02) for general health. Females had statistically lower SF-36 scores than males in the domains Bodily pain (P = 0.02), Vitality (P 0.001), and Mental Health (P 0.001). G1 showed lower scores in the domains Limitations due to Emotional Problems (P = 0.008) and Mental Health (P = 0.036). However, when the confounding factors (age and gender) were analyzed, the oral cleft type did not affect health-related quality of life (P 0.05). Considering the age and gender, the cleft lip and palate types did not affect the health-related quality of life of adolescents.
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- 2019
7. Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies?
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Daniela Rios, Daniela Alejandra Cusicanqui Méndez, Giuliana Campos Chaves Lamarque, Evandro José Dionísio, Thais Marchini de Oliveira, Eliezer Gutierrez, Maria Aparecida de Andrade Moreira Machado, and Thiago Cruvinel
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Curcumin ,medicine.medical_treatment ,DENTINA ,030303 biophysics ,Biophysics ,Positive control ,Photodynamic therapy ,Dermatology ,Dental Caries ,Plaque control ,Microbiology ,Streptococcus mutans ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Pharmacology (medical) ,Statistical analysis ,Child ,0303 health sciences ,Microbial Viability ,Photosensitizing Agents ,Dose-Response Relationship, Drug ,Chemistry ,Stem Cells ,Chlorhexidine ,Biofilm ,Antimicrobial ,In vitro ,Lactobacillus ,Photochemotherapy ,Oncology ,Research Design ,Biofilms ,Dentin ,medicine.drug - Abstract
Background: Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. The aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24 h later). Methods: Dentin caries microcosms were grown on bovine dentin discs (37 °C, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 μM curcumin and 75 J.cm−² LED, or 0.06% and 0.12% CHX. Then, total microorganisms, total streptococci, mutans streptococci, and total lactobacilli counts were determined. The statistical analysis was conducted by Kruskal-Wallis and post-hoc Dunn's tests (P Results: Curcumin-mediated aPDT (C + L+), 0.06% and 0.12% CHX reduced mutans streptococci counts (0.19, 0.10 and 0.07 log10 respectively) in the immediate analysis. After 24 h, it was observed a re-growth of microorganisms treated by curcumin-mediated aPDT, whereas both CHX concentrations demonstrated a decrease of the viable microorganisms. Conclusion: This study confirmed the substantive effect of CHX and the immediate effect of aPDT. The use of a neutralizer solution was important to block the substantivity of CHX and permit its fair comparison with aPDT, allowing its use as a positive control in further studies.
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- 2019
8. Dental Plaque Disclosing as an Auxiliary Method for Professional Dental Prophylaxis in Early Childhood
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Heitor Marques Honório, Celso Dv Tripodi, Daniela Rios, Franciny Querobim Ionta, Thais Marchini de Oliveira, Catarina Rb de Alencar, Priscilla Sp Gonçalves, and Gabriela Cristina de Oliveira
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PLACA BACTERIANA ,Preventive dentistry ,Dentistry ,Orthodontics ,Dental plaque ,03 medical and health sciences ,Professional education ,0302 clinical medicine ,Coloring agents ,stomatognathic system ,medicine ,Clinical significance ,Early childhood ,030219 obstetrics & reproductive medicine ,business.industry ,Dental Prophylaxis ,Dental Care for Children ,Dental prophylaxis ,030206 dentistry ,Preventive Dentistry ,medicine.disease ,Clinical trial ,stomatognathic diseases ,Pediatrics, Perinatology and Child Health ,Periodontics ,Original Article ,Oral Surgery ,business ,Early childhood caries ,Dental care for children - Abstract
Aim This study aimed to clarify whether dental plaque disclosing before professional prophylaxis would improve the visualization of dental plaque, therefore optimizing the procedure. Materials and methods The sample comprised both the smooth and occlusal surfaces of the teeth of 25 children from 18 to 36 months. A randomized crossover clinical trial was conducted with two groups: GI-with disclosing and GII-without disclosing (washout/1 month). The teeth were stained with 1% malachite green solution to assess the amount of dental plaque on smooth and occlusal surfaces. Results Dental plaque removal was more effective and statistically faster (391.27 seconds ±142.2) in GI. The plaque index and the prophylaxis duration were inversely related in GI. The child's behavior did not affect the plaque removal effectiveness, regardless of plaque disclosure (p > 0.05). Conclusion Dental plaque disclosing in young children is an effective and less time-consuming method that improves the quality of the professional dental prophylaxis. Clinical significance Biofilm control is important for the success of programs for prevention of early childhood caries (ECC) and considering the particularities inherent to the dental care of infants, it is necessary that periodic professional prophylaxis is effective in eliminating the oral biofilm, ideally reducing chair time. Although dental plaque disclosing has been traditionally performed on educational basis during oral hygiene instruction, this resource can be safely employed to help professional prophylaxis in infants. How to cite this article de Alencar CRB, de Oliveira GC, et al. Dental Plaque Disclosing as an Auxiliary Method for Professional Dental Prophylaxis in Early Childhood. Int J Clin Pediatr Dent 2019;12(3):189-193.
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- 2019
9. Effect of palm oil alone or associated to stannous solution on enamel erosive-abrasive wear: A randomized in situ/ex vivo study
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Heitor Marques Honório, Daniela Rios, N. M. Santos, Franciny Querobim Ionta, P. P. Val, Thais Marchini de Oliveira, B. T. P. Bergantin, and Catarina Ribeiro Barros de Alencar
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Adult ,Male ,0301 basic medicine ,In situ ,business.product_category ,Adolescent ,In Vitro Techniques ,Palm Oil ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Animal science ,Palm oil ,Animals ,Humans ,Single-Blind Method ,Tooth Erosion ,General Dentistry ,Cross-Over Studies ,Toothpaste ,Enamel paint ,Abrasive ,030206 dentistry ,Cell Biology ,General Medicine ,Demineralization ,Tooth Abrasion ,030104 developmental biology ,Otorhinolaryngology ,chemistry ,visual_art ,Tin Fluorides ,visual_art.visual_art_medium ,Cattle ,Female ,ESMALTE DENTÁRIO ,Citric acid ,business ,Ex vivo - Abstract
Palm oil has demonstrated preventive potential against initial erosive demineralization in vitro. This in situ study evaluated the effect of palm oil alone or associated with stannous-containing (Sn) solution on preventing enamel loss from an erosive/abrasive challenge.This single-blind, randomized, crossover in situ/ex vivo study was developed in four phases (one per group of five days) with sixteen volunteers. Enamel blocks (n = 256) were allocated to groups according to the treatment: Palm oil; Palm oil plus Sn solution; Sn solution - positive control; and Deionized water - negative control. Half of the enamel blocks of each group was subjected to erosion and the other half to erosion + abrasion. The daily ex vivo protocol consisted of four citric acid immersions (2 min). Before the first and third acid exposure, the blocks were treated with the test solutions (1 drop/block) for 1 min followed by acid immersion and abrasive challenge (toothpaste was applied on all blocks and half were brushed for 15 s/block). Enamel loss was quantified profilometrically and data were analyzed by two-way ANOVA and Tukey's test (p 0.05).A significant difference was found for type of treatment (p 0.001); wear condition (p = 0.38) and the treatment x condition interaction (p = 0.33) was non-significant. Palm oil associated or not to Sn solution significantly reduced enamel wear in comparison with the negative control but did not differ from the positive control (p 0.05).Palm oil was able to prevent enamel loss under erosive and erosive + abrasive challenges in a similar extend to stannous-containing commercial solution.
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- 2018
10. Arch Asymmetry in Patients With Cleft Lip and Palate After Rehabilitation Treatment Using Stereophotogrammetry
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Thais Marchini de Oliveira, Jefferson Freire Cardoso, Maria Giulia Rezende Pucciarelli, Simone Soares, Karin Hermana Neppelenbroek, and Guilherme Hideki de Lima Toyoshima
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Adult ,Adolescent ,Cleft Lip ,medicine.medical_treatment ,media_common.quotation_subject ,Asymmetry ,Young Adult ,03 medical and health sciences ,Dental Arch ,0302 clinical medicine ,Female patient ,Maxilla ,medicine ,Humans ,In patient ,Arch ,030223 otorhinolaryngology ,media_common ,Orthodontics ,Interdisciplinary treatment ,Rehabilitation ,business.industry ,ESTÉTICA ,030206 dentistry ,General Medicine ,Cleft Palate ,Otorhinolaryngology ,Photogrammetry ,Female ,Surgery ,business ,Student's t-test - Abstract
Arch asymmetry in cleft patients is a current problem that interdisciplinary treatment aims to solve. This research proposed to analyze the final rehabilitation, according to the arch symmetry of these patients. Thirty-five patients aged between 18 and 30 years, rehabilitated with a fixed partial denture or implants in the cleft area. The analysis was performed using digitalized dental casts with a laser model scanner (R700TM; 3Shape A/S, Holmens Kanal 7, 1060, Copenhagen/Denmark), analyzed with a Vectra Analysis Module software program (VECTRA H1; Canfield Scientific, 4 Wood Hollow Road, Parsippany, NJ 07054). Three linear measurements were evaluated, incisal-canine, canine-molar, and incisal-molar distance. The Student t test was applied to test the significance (P = 0.05) of an observed sample by correlation coefficient test (r-value). Female patients showed a significant correlation in arch symmetry. According to the rehabilitation treatment, patients who received implants showed a high correlation and significant symmetry at all maxillary distances. Finally, according to the cleft side in the maxillary dimensions, even though the majority of patients had clefts on the left side, only patients with a cleft on the right side showed symmetry in this area. Patients rehabilitated with implants in the cleft area showed a more symmetrical maxillary arch than those restored with fixed partial dentures.
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- 2021
11. Pulp liner materials in selective caries removal: study protocol for a randomised controlled trial
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Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Daniela Rios, Natalino Lourenço Neto, Thiago Cruvinel, Tássia Carina Stafuzza, Luciana Lourenço Ribeiro Vitor, Vivien Thiemy Sakai, and Ana Beatriz Silveira Moretti
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Molar ,protocols & guidelines ,Dental Caries Susceptibility ,lcsh:Medicine ,Dentistry ,Dentistry and Oral Medicine ,Dental Caries ,law.invention ,quality in health care ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,stomatognathic system ,law ,Medicine ,Humans ,030212 general & internal medicine ,ENSAIO CLÍNICO CONTROLADO RANDOMIZADO ,Tooth, Deciduous ,Randomized Controlled Trials as Topic ,Protocol (science) ,business.industry ,Periapical radiography ,lcsh:R ,Reproducibility of Results ,paediatric oral & maxillofacial surgery ,030206 dentistry ,General Medicine ,Second primary cancer ,Clinical trial ,stomatognathic diseases ,Pulp (tooth) ,business ,Caries Removal ,Brazil - Abstract
IntroductionThe literature shows that selective carious tissue removal (SCTR) decreases the number and diversity of bacteria, stops the caries process and reduces the risk of pulp exposure. However, no consensus exists on which pulp liner would be suitable for teeth undergoing SCTR. So, this study will verify the in vivo response of dentine–pulp complex after SCTR in primary teeth with or without pulp liner material.Methods and analysisA randomised clinical trial, double-blinded, parallel-group and allocation concealment will be conducted with the enrolment of 384 patients from 5 to 9 years, with one maxillary/mandibular first/second primary molars with deep occlusal/occlusoproximal cavities. The remaining dentine will be lined with calcium hydroxide cement—group 1; mineral trioxide aggregate—group 2 and without liner—group 3. The primary outcome will be success of the of dentine–pulp complex evaluated clinically and radiographically at 6, 12 and 24 months, while the secondary outcomes will be the measurement of the dentine barrier on periapical radiographs. During all study, two trained and calibrated examiners will evaluate the treated teeth clinically and radiographically. Interexaminer and intraexaminer reliability will be verified by casual and systematic error. The Kolmogorov–Smirnov test will be adopted to test the normality of continuous variables. Comparisons among groups will be performed by using the χ2 test and anaylsis of variance, followed by Tukey test (pEthics and disseminationThe present protocol was submitted and approved by the Ethical Committee of the University of São Paulo, Bauru, São Paulo, Brazil (CAAE: 79123517.0.0000.5417). Consent for publication will be obtained from all parents or legal guardians. Results of this study will be reported in full through peer-reviewed journals.Trial registration numberRBR-9fsxnn.
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- 2021
12. Prospective cohort 3D study of dental arches in children with bilateral orofacial cleft: Assessment of volume and superimposition
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Chiarella Sforza, Simone Soares, Thais Marchini de Oliveira, Marcio De Menezes, Maria Aparecida de Andrade Moreira Machado, Eloá Cristina Passucci Ambrosio, and Cleide Felício de Carvalho Carrara
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medicine.medical_treatment ,Cleft Lip ,03 medical and health sciences ,0302 clinical medicine ,Dental Arch ,stomatognathic system ,medicine ,Maxilla ,Superimposition ,Humans ,030212 general & internal medicine ,Prospective Studies ,Prospective cohort study ,Child ,General Dentistry ,Orthodontics ,business.industry ,Dental Models ,030206 dentistry ,ESTUDOS DE COORTES ,Cleft Palate ,stomatognathic diseases ,Dental arch ,medicine.anatomical_structure ,Palatoplasty ,Bilateral cleft lip ,Cheiloplasty ,business ,Cohort study - Abstract
Cohort studies have evaluated dental arches of children.To evaluate the volumetric, linear, palatal surface area, and the dental arch superimposition of participants with bilateral complete cleft lip (BCL) and bilateral cleft lip and palate (BCLP) surgically treated in a specialized hospital.One hundred and thirty six digitized dental models evaluated before cheiloplasty (T1), after cheiloplasty (T2), and after palatoplasty (T3). The stereophotogrammetry software analysed the volume, palate superimposition, linear, and area measurements.In BCL group, at T2, C-C', T-T', area, and volume significantly increased (P = .000, P .000, P = .010 e P = .003, respectively). In BCLP group, the comparison T3 × T1 showed that C-C' decreased, whereas T-T' and the area increased (P .000, P .000, P = .000). The volume increased at T2, but decreased at T3 (P .000) in participants with BCLP. The intergroup analysis revealed that C-C', T-T', I-C', and I-C were significantly smaller in participants with BCLP (P .000, P = .016, P = .001 e P = .020, respectively), whereas the volume, superimposition, and area were statistically similar between groups (P .05).The comparison between bilateral orofacial clefts showed reduction in the transversal and anteroposterior linear measurements, but not in the area and volume, which was confirmed by the superimposition of the dental arches.
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- 2020
13. Assessment of dental arch stability after orthodontic treatment and oral rehabilitation in complete unilateral cleft lip and palate and non-clefts patients using 3D stereophotogrammetry
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Simone Soares, Thais Marchini de Oliveira, Maria Giulia Rezende Pucciarelli, Heitor Marques Honório, Guilherme Hideki de Lima Toyoshima, and Chiarella Sforza
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Adult ,Adolescent ,medicine.medical_treatment ,Cleft Lip ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,medicine ,Maxilla ,Humans ,Statistical analysis ,In patient ,3d stereophotogrammetry ,Arch ,030223 otorhinolaryngology ,General Dentistry ,Retrospective Studies ,Orthodontics ,Orthodontic appliances ,Rehabilitation ,Dental arch ,business.industry ,Significant difference ,030206 dentistry ,lcsh:RK1-715 ,Cleft Palate ,medicine.anatomical_structure ,lcsh:Dentistry ,Photogrammetry ,Oral and maxillofacial surgery ,Three-dimensional imaging ,business ,TRATAMENTO ORTODÔNTICO ,Research Article - Abstract
Background Although arch stability has been studied in patients without a cleft, evidence for patients with a cleft is sparse. Therefore, we compared the dimensions and stability of dental arches in cleft lip and palate patients and those without a cleft. Methods Forty participants, 20 with a complete unilateral cleft lip and palate and 20 non-cleft patients aged from 18 to 30 years, with anterior and/or posterior crossbite and receiving orthodontic treatment were evaluated retrospectively. Eighty gypsum casts were digitized using a laser model scanner casts for both groups made immediately after the orthodontic treatment was completed (T1). Also, for the Cleft Lip and Palate group, casts were obtained and digitized 1 year after implant-supported rehabilitation (T2) and for the Non-Cleft Lip and Palate group, 1 year after the conclusion of the orthodontic treatment (T2). The formula: Δ = T2-T1 evaluated the stability of dental arches for inter-canine distances (C-C′), inter-molar distances (M-M’), arch length (I-M), palate surface and volume. The dimensions of the dental arches were measured digitally. The independent t test was used for statistical analysis (α = 0.05). Results A statistical difference was found in the stability of the groups for inter-canine (cleft area) measurement. At the times T1 and T2, a statistically significant difference was found in the arch length, surface and volume. Conclusions This study concluded that in the Cleft Lip and Palate group, the maxillary dimensions were not stabilized after 1 year of orthodontic and prosthodontic treatment (mainly for the inter-canine linear measurement) and that the transverse arch dimensions were smaller compared with those of non-cleft patients.
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- 2020
14. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?
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Natalino Lourenço Neto, Thiago José Dionísio, L. L. R. Vitor, Mariel Tavares Oliveira Prado Bergamo, Eloá Cristina Passucci Ambrosio, Maria Aparecida de Andrade Moreira Machado, Vivien Thiemy Sakai, Nádia Carolina Teixeira Marques, Carlos Ferreira dos Santos, Thais Marchini de Oliveira, and Rodrigo Cardoso de Oliveira
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Chemistry ,Cell Survival ,030206 dentistry ,Dermatology ,TERAPIA FOTODINÂMICA ,Vegf mrna ,Andrology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Vascular endothelial growth factor A ,0302 clinical medicine ,medicine.anatomical_structure ,Cell culture ,Gene expression ,medicine ,Pulp (tooth) ,Humans ,Surgery ,Viability assay ,Irradiation ,Low-Level Light Therapy ,Tooth, Deciduous ,Fibroblast ,Dental Pulp ,Cell Proliferation - Abstract
This study aimed to evaluate the effect of different photobiomodulation (PBM) radiant exposures on the viability, proliferation, and gene expression of pulp fibroblasts from human primary teeth (HPF) involved in the pulp tissue repair. HPF were irradiated with Laser InGaAlP (Twin Flex Evolution, MMOptics®) at 660-nm wavelength (red); single time, continuous mode, 0.04-cm2 laser tip area, and 0.225-cm laser tip diameter, keeping the distance of 1 mm between the laser beam and the cell culture. The doses used were between 1.2 and 6.2 J/cm2 and were evaluated at the 6 h, 12 h, and 24 h after PBM. MTT and crystal violet assays evaluated the cell viability and proliferation. RT-PCR verified VEGF and FGF-2 mRNA expression. A blinded examiner analyzed the data through two-way ANOVA followed by Tukey test (p < 0.05). The groups with higher powers (10 mW, 15 mW, 20 mW, and 25 mW), shortest application periods (10 s), and radiant exposures between 2.5 and 6.2 J/cm2 exhibited statistically higher viability than that of the groups with small power (5 mW), longer application period (50 s), and radiant exposure of 6.2 J/cm2 (p < 0.05). VEGF and FGF-2 mRNA expression were observed at the three evaluated periods (6 h, 12 h, and 24 h) and the highest expression was in the shortest period (p < 0.05). All radiant exposures maintained HPF viable. The period of 6 h after irradiation showed statistically greater gene expression for both growth factors than other periods. VEGF mRNA had no differences among the dosimetries studied. The best radiant exposures for FGF-2 gene expression were 2.5 J/cm2 and 3.7 J/cm2.
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- 2020
15. Parental-oriented educational mobile messages to aid in the control of early childhood caries in low socioeconomic children: a randomized controlled trial
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Maria Aparecida de Andrade Moreira Machado, Matheus Lotto, Daniela Rios, Thais Marchini de Oliveira, Patricia Estefania Ayala Aguirre, Anna Paola Strieder, and Thiago Cruvinel
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Parents ,Gerontology ,Dental Caries Susceptibility ,media_common.quotation_subject ,Early Childhood Caries ,Dental Caries ,Article ,Literacy ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,McNemar's test ,Randomized controlled trial ,law ,Intervention (counseling) ,eHealth ,medicine ,Humans ,030212 general & internal medicine ,ENSAIO CLÍNICO CONTROLADO RANDOMIZADO ,Child ,General Dentistry ,Socioeconomic status ,mHealth ,media_common ,business.industry ,030206 dentistry ,medicine.disease ,Child, Preschool ,Randomized Controlled Trial ,Health Education, Dental ,sense organs ,business ,Brazil ,Early childhood caries - Abstract
Highlights • Educational mobile text messages were effective to control the severity of ECC. • They influenced changes in parental reports about children’s sugar consumption. • Also, they seemed beneficial to improve parental electronic health literacy levels., Objectives To evaluate the effectiveness of educational messages as an aid in the control of early childhood caries (ECC) in low socioeconomic children. Methods A single-blinded, randomized, and parallel-group study was conducted with 104 dyads of parents and children aged between 36-60 months, recruited in preschools from (blinded). The participants were randomly allocated into control and intervention groups (1:1), stratified by eHealth literacy scores (eHEALS) of parents and caries experience of children. Each 2 weeks, text messages were sent to parents of intervention group via WhatsApp. Visible plaque index (VPI) and the International Caries Detection and Assessment System (ICDAS) were assessed at baseline, 3- and 6-month follow-up, while eHEALS and dietary habits were determined at baseline and 6-month follow-up. Statistical analysis was performed to intra and intergroup comparisons through Fischer´s exact and McNemar tests, and Mann-Whitney U and Friedman tests, respectively (P
- Published
- 2020
16. Pulp repair response after the use of a dentin-pulp biostimulation membrane (BBio) in primary teeth: study protocol for a randomized clinical trial
- Author
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Natalino Lourenço Neto, Silgia Aparecida da Costa, Tássia Carina Stafuzza, Maria Aparecida de Andrade Moreira Machado, Luciana Lourenço Ribeiro Vitor, Thais Marchini de Oliveira, and Sirlene Maria da Costa
- Subjects
Male ,Mineral trioxide aggregate ,Pulpotomy ,Medicine (miscellaneous) ,Dentistry ,Pulp ,02 engineering and technology ,Dental Caries ,law.invention ,Study Protocol ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Randomized controlled trial ,law ,Dentin ,medicine ,Humans ,Pharmacology (medical) ,Tooth, Deciduous ,ENSAIO CLÍNICO CONTROLADO RANDOMIZADO ,Child ,Randomized Controlled Trials as Topic ,Chitosan ,lcsh:R5-920 ,business.industry ,Oxides ,Deciduous ,030206 dentistry ,Odds ratio ,021001 nanoscience & nanotechnology ,Confidence interval ,Clinical trial ,Drug Combinations ,stomatognathic diseases ,Treatment Outcome ,medicine.anatomical_structure ,Child, Preschool ,Pulp (tooth) ,Female ,Vital pulp therapy ,lcsh:Medicine (General) ,0210 nano-technology ,business ,Tooth ,Brazil - Abstract
Background Vital pulp therapy aims at maintaining the pulp tissue injured but vital. Thus, the use of capping materials that induce tissue regeneration is a great current trend. This study aims to evaluate clinically and radiographically the pulp repair after the use of dentin-pulp biostimulation membrane in primary teeth. Methods Four hundred and sixty-eight teeth from children aged between 5 and 9 years old, both genders, with deep caries lesion with pulp involvement, but no furcal impairment and any sign of necrosis will be selected. The vital pulp therapy will be performed with mineral trioxide aggregate (control group) and dentin-pulp biostimulation chitosan membrane (BBio group). The clinical and radiographic outcomes will be assessed at 12 and 24 months after treatment. The thickness of the dentin barrier will be verified through Image J2 software. The Wilcoxon signed rank test and Mann-Whitney test will respectively compare the intra- and intergroup clinical and radiographic outcomes. Paired t test and independent t test will respectively compare the intra- and intergroup radiographic measurements. The logistic regression will be applied, and the degrees of this association will be measured using odds ratio (OR) and 95% confidence interval (95% CI). Discussion Therefore, this study protocol aims at new perspectives of vital pulp therapy of primary teeth by employing new easy-handling, low-cost material to keep viable the pulp tissue capable of regenerating and maintain the physiological process of deciduous tooth exfoliation. Trial registration Brazilian Registry of Clinical Trials RBR-6vr58b. Registered on 17 February 2019.
- Published
- 2020
17. Dental anomaly pattern and multiple ectopic teeth
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Marilia Yatabe, Guilherme Janson, Thais Marchini de Oliveira, Camila Massaro, Louise Resti Calil, and Daniela Gamba Garib
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Orthodontics ,Panoramic radiograph ,Dentition ,business.industry ,030206 dentistry ,Overbite ,medicine.disease ,stomatognathic diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,stomatognathic system ,Incisor ,Curve of Spee ,Maxilla ,Lingual arch ,medicine ,MALOCLUSÃO DE ANGLE CLASSE II ,Malocclusion ,business ,030217 neurology & neurosurgery - Abstract
Treatment for a patient with a dental anomaly pattern is challenging and complex. This article presents a rare case with an association of multiple ectopic eruptions treated in 2 phases. An 8-year-old boy in the early mixed dentition showed a Class II, Division 2 malocclusion, and an ectopic eruption of the mandibular right lateral incisor. The treatment plan included extractions of the deciduous mandibular right lateral incisor and canine, followed by partial mandibular fixed appliances and a lingual arch. In the late mixed dentition, the panoramic radiograph showed bilateral ectopic eruptions of the maxillary canines. The deciduous maxillary canines were extracted, and a cervical headgear was installed. Within 12 months, both permanent maxillary canines spontaneously erupted. Comprehensive orthodontic treatment was initiated when the patient was aged 13 years to level the curve of Spee and reduce the overbite. Phase 2 lasted 15 months. In patients with dental anomaly patterns, longitudinal follow-up during the mixed dentition is highly important and might contribute to simplifying comprehensive orthodontic treatment and reducing complications and adverse effects.
- Published
- 2020
18. Post-surgical effects on the maxillary segments of children with oral clefts: New three-dimensional anthropometric analysis
- Author
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Thais Marchini de Oliveira, Marcio De Menezes, Maria Aparecida de Andrade Moreira Machado, Eloá Cristina Passucci Ambrosio, Chiarella Sforza, and Cleide Felício de Carvalho Carrara
- Subjects
Male ,Post surgical ,Wilcoxon signed-rank test ,Cleft Lip ,medicine.medical_treatment ,03 medical and health sciences ,Imaging, Three-Dimensional ,0302 clinical medicine ,Maxilla ,medicine ,Humans ,030223 otorhinolaryngology ,ANTROPOMETRIA ,Orthodontics ,Anthropometry ,business.industry ,Infant ,030206 dentistry ,Unilateral complete cleft lip ,Cleft Palate ,Treatment Outcome ,Palatoplasty ,Otorhinolaryngology ,Female ,Surgery ,Anatomic Landmarks ,Oral Surgery ,Cheiloplasty ,business ,Software ,Student's t-test - Abstract
This study aimed to use new three-dimensional (3D) anthropometric analyses to verify the post-surgical effects on the maxillary segments of children with unilateral cleft lip and palate. The sample was composed by digitized dental models of 60 children with unilateral complete cleft lip and alveolus (UCLA) and complete unilateral cleft lip and palate (UCLP). The impressions were taken before cheiloplasty (T1), after cheiloplasty (T2), and after palatoplasty (T3). The 3D anthropometric analyses of digitized dental casts were obtained through a specific software. Intragroup changes were applied paired t test and Wilcoxon test (UCLA group) and for the UCLP group, repeated-measures analyses of variance followed by the Tukey test. For intergroup analyses, an independent t test and Mann-Whitney test were used. The palatal dimensional changes of UCLA group showed that the distances IC, I-T', and I-T significantly increased after cheiloplasty (p = 0.0002, p = 0.0007 and p
- Published
- 2018
19. CD31 expression in human primary teeth treated with photobiomodulation therapy
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Carlos Ferreira dos Santos, Natalino Lourenço Neto, Maria Aparecida de Andrade Moreira Machado, Nádia Carolina Teixeira Marques, Luciana Lourenço Ribeiro Vitor, Vivien Thiemy Sakai, Camila Oliveira Rodini, M. T. O. Prado, and Thais Marchini de Oliveira
- Subjects
Molar ,CD31 ,Pathology ,medicine.medical_specialty ,Leukocyte migration ,business.industry ,Angiogenesis ,Pulpotomy ,Inflammation ,030206 dentistry ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Medicine ,Pulp (tooth) ,medicine.symptom ,business ,Immunostaining - Abstract
This study aimed to evaluate the effects of photobiomodulation (PBM) on CD31 expression in pulp tissue repair of human primary teeth. Fifteen mandibular primary molars were divided into the following groups: GI—calcium hydroxide (CH), GII—PBM + CH, and GIII—PBM + zinc oxide/eugenol (ZOE). In the GII and GII groups, an indium–gallium–aluminum phosphide (InGaAlP) diode laser was used for the irradiation through 320-μm diameter optical fiber in contact with pulp tissue. The red laser diode parameters were set at 660-nm wavelength, 10-mW power output, 2.5-J/cm2 energy density, 50–60-Hz frequency, output beam area of 0.04 cm2, and irradiation time of 10 s in continuous mode. After pulpotomy treatment, clinical and radiographic follow-ups were accomplished until the teeth achieved regular exfoliation period. Teeth were extracted for histological analysis and immunolocalization of CD31. Histopathological statistical analyses were performed by Kruskal-Wallis followed by Dunn test to determine statistically significant differences (p
- Published
- 2018
20. Relapse after Le Fort I surgery in oral cleft patients: a 2-year follow-up using digitized and 3D models
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Renato Yassutaka Faria Yaedú, Simone Soares, W. S. Silva, Ana Lúcia Pompéia Fraga de Almeida, Juliana Dreyer da Silva de Menezes, Maria Giulia Rezende Pucciarelli, Flavia Maria Ravagnani Neves Cintra, Karin Hermana Neppelenbroek, and Thais Marchini de Oliveira
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Cephalometry ,medicine.medical_treatment ,Orthognathic surgery ,03 medical and health sciences ,Imaging, Three-Dimensional ,0302 clinical medicine ,Recurrence ,medicine ,Humans ,Osteotomy, Le Fort ,Craniofacial ,030223 otorhinolaryngology ,General Dentistry ,Oral cleft ,business.industry ,Dental Models ,030206 dentistry ,Maxillary Osteotomy ,Craniometry ,Surgery ,Cleft Palate ,CIRURGIA ORTOGNÁTICA ,Oral and maxillofacial surgery ,Female ,business ,Cephalogram ,Follow-Up Studies - Abstract
This retrospective observational study aimed to evaluate and identify the relapse rate after orthognathic surgery for maxillary advancement (Le Fort I maxillary osteotomy) in oral cleft patients through digitized cephalograms and 3D dental models, following 2 years. Lateral cephalograms and dental casts of 17 individuals, enrolled in Orthodontics Department in Hospital of Rehabilitation of Craniofacial Anomalies, were carried out. The digital cephalometric tracings were evaluated in: T1-before surgery, T2-immediate after surgery, T3-6-month to 1-year after surgery. The dental study casts were digitized and evaluated in: F1-before surgery; F2-3-month to 1-year after surgery; F3-1 to 2 years after surgery. The analyses of the dental arches were performed directly on the scanned images. A single examiner previously trained and calibrated performed all the assessments. Repeated measures ANOVA was applied to study the variables and compare the periods, followed by Tukey test to evaluate the statistically significant differences, with level of significance of 5%. The digital cephalogram results showed that the vertical movement statistically differed from T2 to T3 (p = 0.002). The right and left premolar relationship in digitized models revealed that at F2 the individuals exhibited ¼ Class II and Class I, in 29.4 and 23.5% of the cases, respectively; and at F3, Class I, 58.8 and 70.6% of the cases, respectively. The cephalometry showed the relapse in the vertical movement after orthognathic surgery for maxillary advancement, but no relapse in the other evaluated parameters.
- Published
- 2018
21. Clinical, Radiographic and Histological Evaluation of Primary Teeth Pulpotomy Using MTA And Ferric Sulfate
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Leopoldo Cosme-Silva, Marina Azevedo Junqueira, Ana Beatriz Silveira Moretti, Thais Marchini de Oliveira, Nayara Nery Oliveira Cunha, Nádia Carolina Teixeira Marques, Fernanda Ferreira Caixeta, and Vivien Thiemy Sakai
- Subjects
Male ,Molar ,Mineral trioxide aggregate ,Radiography ,Radiodensity ,Pulpectomy ,Pulpotomy ,Dentistry ,Connective tissue ,Ferric Compounds ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Tooth pulp stimulation ,Radiography, Dental ,medicine ,Humans ,Tooth, Deciduous ,pulpotomy ,Aluminum Compounds ,Child ,deciduous tooth ,General Dentistry ,Dental Pulp ,mineral trioxide aggregate ,MOLAR ,business.industry ,Silicates ,Oxides ,030206 dentistry ,Calcium Compounds ,Drug Combinations ,stomatognathic diseases ,Treatment Outcome ,medicine.anatomical_structure ,Child, Preschool ,Pulp (tooth) ,Female ,business ,Pulp Capping and Pulpectomy Agents ,ferric sulfate ,030217 neurology & neurosurgery - Abstract
The aim of this study was to evaluate the clinical, radiographic and histological outcomes of the dentin-pulp complex from primary molars after pulpotomy with mineral trioxide aggregate (MTA) and 15.5% ferric sulfate (FS). Thirty-one primary molars were randomly allocated into MTA or FS groups. Clinical and radiographic evaluations were recorded at 3-, 6-, 12- and 18-month follow-up. Teeth at the regular exfoliation period were extracted and processed for histological analysis. Clinical and radiographic data were tested by statistical analysis (p≤0.01). Histological outcomes were analyzed descriptively. All of the treated teeth presented clinical success over the experimental periods. Both groups exhibited 100% of radiographic success at 3, 6 and 12 months. At the 18-month follow-up, one tooth from FS group presented inter-radicular radiolucency (p>0.01). Histologically, the treated teeth presented pulp vitality and absence of inflammatory infiltrate into the connective tissue. Only MTA group showed hard tissue barrier surrounded by odontoblasts over the pulp stumps. Both MTA and 15.5% FS are effective for pulpotomies of primary teeth. Although MTA is considered the first-choice material, FS may be a suitable alternative when treatment cost is an issue. Resumo O objetivo deste estudo foi avaliar os resultados clínicos, radiográficos e histológicos do complexo dentino-pulpar de molares decíduos após pulpotomia com agregado trióxido mineral (MTA) e sulfato férrico (SF) 15,5%. Trinta e um molares decíduos foram alocados aleatoriamente nos grupos MTA ou SF. As avaliações clínicas e radiográficas foram realizadas aos 3, 6, 12 e 18 meses de acompanhamento. Os dentes no período regular de esfoliação foram extraídos e processados para análise histológica. Os dados clínicos e radiográficos foram analisados estatisticamente (p≤0,01). O resultados histológicos foram analisados descritivamente. Todos os dentes tratados apresentaram sucesso clínico ao longo dos períodos experimentais. Ambos os grupos exibiram 100% de sucesso radiográfico aos 3, 6 e 12 meses. Aos 18 meses de acompanhamento, um dente do grupo SF apresentou radiolucidez interradicular (p>0,01). Histologicamente, os dentes tratados apresentaram vitalidade pulpar e ausência de infiltrado inflamatório no tecido conjuntivo. Somente o grupo MTA mostrou barreira de tecido duro rodeada por odontoblastos sobre os cotos pulpares. MTA e SF 15,5% são eficazes para pulpotomias de dentes decíduos. Embora o MTA seja considerado o material de primeira escolha, o SF pode ser uma alternativa adequada quando o custo do tratamento é um problema.
- Published
- 2018
22. Acquired Enamel Pellicle Engineered Peptides: Effects on Hydroxyapatite Crystal Growth
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Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Yizhi Xiao, Walter L. Siqueira, Eduardo Buozi Moffa, Maria Teresa Valente, and Karla Tonelli Bicalho Crosara
- Subjects
0301 basic medicine ,Protein domain ,lcsh:Medicine ,Peptide ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Engineering ,Protein Domains ,Gene duplication ,medicine ,Dental Pellicle ,Dental Enamel ,lcsh:Science ,IC50 ,Histatin 3 ,REMINERALIZAÇÃO DENTÁRIA ,chemistry.chemical_classification ,Multidisciplinary ,Enamel paint ,Calculus (dental) ,lcsh:R ,030206 dentistry ,medicine.disease ,030104 developmental biology ,Durapatite ,chemistry ,Biochemistry ,visual_art ,visual_art.visual_art_medium ,lcsh:Q ,Growth inhibition ,Crystallization ,Peptides - Abstract
The aim of this study was to test the hypothesis that duplication/hybridization of functional domains of naturally occurring pellicle peptides amplified the inhibitory effect of hydroxyapatite crystal growth, which is related to enamel remineralization and dental calculus formation. Histatin 3, statherin, their functional domains (RR14 and DR9), and engineered peptides (DR9-DR9 and DR9-RR14) were tested at seven different concentrations to evaluate the effect on hydroxyapatite crystal growth inhibition. A microplate colorimetric assay was used to quantify hydroxyapatite crystal growth. The half-maximal inhibitory concentration (IC50) was determined for each group. ANOVA and Student-Newman-Keuls pairwise comparisons were used to compare the groups. DR9-DR9 increased the inhibitory effect of hydroxyapatite crystal growth compared to single DR9 (p
- Published
- 2018
23. Clinical and Radiographic Success of Selective Caries Removal to Firm Dentin in Primary Teeth: 18-Month Follow-Up
- Author
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Daniela Rios, Thiago Cruvinel da Silva, Thais Marchini de Oliveira, Tássia Carina Stafuzza, Luciana Lourenço Ribeiro Vitor, and Maria Aparecida de Andrade Moreira Machado
- Subjects
Mineral trioxide aggregate ,Radiography ,Glass ionomer cement ,Dentistry ,Case Report ,Mandibular first molar ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Randomized controlled trial ,law ,Dentin ,Medicine ,030212 general & internal medicine ,General Dentistry ,business.industry ,RK1-715 ,030206 dentistry ,MANDÍBULA ,stomatognathic diseases ,medicine.anatomical_structure ,Pulp (tooth) ,business ,Caries Removal - Abstract
The selective caries removal is increasingly spreading in daily clinical practice because this minimally invasive technique treats deep carious lesion and decreases the risk of pulp exposure. This case report was aimed at describing the selective removal to firm dentin on the primary mandibular left first molar of a girl aged 7 years and 6 months. The Mineral Trioxide Aggregate (MTA Angelus™) was used as liner, and the tooth was definitively restored with resin-modified glass ionomer cement (Vitremer™). The clinical and radiographic following-up was performed at 6, 12, and 18 months after treatment. The treatment showed satisfactory results after 18-month following-up, suggesting that this minimally invasive approach for carious lesion removal can replace the total removal, when properly indicated. Notwithstanding, further randomized clinical trials with longer following-up periods are still necessary.
- Published
- 2018
24. Storage protocol of dental pulp cells from human exfoliated deciduous teeth
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Natalino Lourenço Neto, Luciana Lourenço Ribeiro Vitor, Letícia Florindo Pereira, Cintia Kazuko Tokuhara, Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Rodrigo Cardoso de Oliveira, Nádia Carolina Teixeira Marques, and M. T. O. Prado
- Subjects
Orthodontics ,business.industry ,Dentistry ,030206 dentistry ,01 natural sciences ,Cryopreservation ,010309 optics ,stomatognathic diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,stomatognathic system ,Cell culture ,0103 physical sciences ,Deciduous teeth ,Medicine ,Pulp (tooth) ,business ,General Dentistry - Abstract
Objective: This study aimed to isolate the cells from the dental pulp tissue of human primary teeth, study the capacity of proliferation, characterize the cells and standardize the technique of culture and expansion to create a cell banking. Material and Methods: Primary teeth with no caries and orthodontic reasons were extracted for pulp tissue obtainment. The cells were extracted from the pulp cells, isolated and cultured under ideal conditions until full expansion. Results: After consecutive passages, the cultured cells were characterized using immunofluorescence technique and frozen between the 2 nd and 6 th passage, thus creating a biorepository of dental pulp cells from human primary teeth. Conclusion: The creation of a cell banking from dental pulp cells from human primary teeth enables the easy application of cells in laboratorial studies, reducing the cost and time for obtaining the samples, avoid the involvement of new subjects and allow a fast reproducibility of the researches. Keywords Cell culture techniques; Tooth, deciduous; Pulp; Fibroblasts; Cryopreservation.
- Published
- 2017
25. Biophysical and biological characterization of intraoral multilayer membranes as potential carriers: A new drug delivery system for dentistry
- Author
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Natalino Lourenço Neto, Thais Marchini de Oliveira, Sirlene Maria da Costa, Rodrigo Cardoso de Oliveira, Silgia Aparecida da Costa, Maria Aparecida de Andrade Moreira Machado, and Mariana Cristina Lobato dos Santos Ribeiro Silva
- Subjects
Materials science ,Absorption of water ,CLOREXIDINA ,Biocompatibility ,Alginates ,Dentistry ,Bioengineering ,02 engineering and technology ,Biomaterials ,Chitosan ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Glucuronic Acid ,Glycerol ,Animals ,MTT assay ,Viability assay ,Drug Carriers ,business.industry ,Hexuronic Acids ,Chlorhexidine ,Membranes, Artificial ,030206 dentistry ,021001 nanoscience & nanotechnology ,Membrane ,chemistry ,Mechanics of Materials ,Delayed-Action Preparations ,Drug delivery ,NIH 3T3 Cells ,0210 nano-technology ,business - Abstract
The current study developed through layer-by-layer deposition a multilayer membrane for intraoral drug delivery and analyzed the biochemical, functional, and biological properties of this membrane. For that purpose, we designed a three-layer chlorhexidine-incorporated membrane composed by pure chitosan and alginate. The biochemical, functional, and biological properties were analyzed by the following tests: degradation in saliva medium; controlled drug release; water absorption, mass loss; pH analysis; and biocompatibility through fibroblast cell viability by MTT assay. All tests were conducted at three different periods (24, 48 and 72 hours). The results demonstrated that hybrid membranes composed by alginate and chitosan with glycerol had greater water absorption and mass loss in buffer solution and in artificial saliva. The controlled drug release test revealed that the hybrid membrane exhibited greater drug release (0.075%). All chlorhexidine-incorporated membranes reduced the cell viability, and chitosan membranes with and without glycerol did not interfere with fibroblast viability. The biochemical and biophysical characteristics of the designed membranes and the findings of cell viability tests indicate great potential for application in Dentistry.
- Published
- 2017
26. Delayed Treatment of Traumatized Primary Teeth with Distinct Pulp Response: Follow-Up until Permanent Successors Eruption
- Author
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Franciny Querobim Ionta, Gabriela Cristina de Oliveira, Catarina Ribeiro Barros de Alencar, Daniela Rios, Thiago Cruvinel, Juliana Calistro da Silva, Thais Marchini de Oliveira, and Priscilla Santana Pinto Gonçalves
- Subjects
genetic structures ,Pulpotomy ,Dentistry ,Case Report ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,EXPOSIÇÃO DA POLPA DENTÁRIA ,medicine ,Maxillary central incisor ,030212 general & internal medicine ,General Dentistry ,Orthodontics ,Dental follicle ,business.industry ,RK1-715 ,030206 dentistry ,Delayed treatment ,medicine.disease ,Periapical lesion ,stomatognathic diseases ,Pulp (tooth) ,Primary Tooth ,business ,Pulp polyp ,psychological phenomena and processes - Abstract
Complicated crown fracture and crown-root fracture with pulp involvement expose dental pulp to the oral environment. The pulp outcome is often unpredictable because the patient and injury which are related to variables can influence the treatment of choice and the prognosis of the case. This report presents the case of a 4-year-old boy with complicated crown fracture with pulp polyp in the primary right maxillary central incisor (51) and crown-root fracture with pulp involvement in the primary left maxillary central incisor (61), which was treated only 3 months after the tooth injuries. The treatment of choice was extraction of tooth (61) due to a periapical lesion with disruption of the dental follicle of the permanent successor and pulpotomy (MTA) of the tooth (51), because the pulp presented signs of vitality. At the follow-up visits, no clinical, symptomalogical, and radiographic changes were observed until the primary tooth’s exfoliation. However, at 3-year follow-up, the permanent successors showed hypocalcification and the position of the permanent right maxillary central incisors (11) was altered. Besides the conservative and adequate delayed treatment, the sequelae on the permanent successors could not be avoided.
- Published
- 2017
27. Do technology-based devices improve carious lesion detection?
- Author
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Daniela Rios, Cleide Felício de Carvalho Carrara, Tatiana Yuriko Kobayashi, Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Luciana Lourenço Ribeiro Vitor, and Thiago Cruvinel da Silva
- Subjects
Orthodontics ,genetic structures ,medicine.diagnostic_test ,business.industry ,Visual examination ,Dentistry ,Physical examination ,030206 dentistry ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,030212 general & internal medicine ,Craniofacial anomaly ,Carious lesion ,business ,Operating microscope ,General Dentistry - Abstract
Objective: To compare the visual-tactile examination with the technology-based caries detection examinations in children. Methods: We assessed the teeth next to the cleft area of 95 children aged 6 to 12 years with oral clefts but without the presence of any associated syndrome or craniofacial anomaly, according to the following detection methods: visual-tactile examination (Method 1), visual-tactile examination through operating microscope (Method 2), visual examination through LED-based fluorescence device (Method 3), using ICDAS-II scores. The caries detection methods were compared by Friedman test (P
- Published
- 2016
28. Are the Internet users concerned about molar incisor hypomineralization? An infoveillance study
- Author
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Patricia Estefania Ayala Aguirre, Thiago Cruvinel, Agnes Fátima Pereira Cruvinel, Anna Paola Strieder, Matheus Lotto, Thais Marchini de Oliveira, and Daniela Rios
- Subjects
Internet ,business.industry ,030206 dentistry ,Molar Incisor Hypomineralization ,Molar ,SAÚDE PÚBLICA ,Incisor ,03 medical and health sciences ,0302 clinical medicine ,Infoveillance ,Prevalence ,Medicine ,Humans ,The Internet ,Dental Enamel Hypoplasia ,030212 general & internal medicine ,Health behavior ,business ,Dental Enamel ,General Dentistry ,Demography - Abstract
BACKGROUND Molar incisor hypomineralization (MIH) is a developmental enamel defect characterized by well-demarcated discolorations frequently detected in molars, causing pain and esthetic alterations. AIM To assess the interests of Google users on MIH-related information. DESIGN Digital data were collected in Google Trends through two search strategies, 'molar incisor hypomineralization' (topic) and 'MIH' (search term), between January 2004 and November 2018. ARIMA models were applied to analyze trends of curves and to predict the activity of Google users during 12 months. Autocorrelation and partial autocorrelation (ACF/PACF) plots were used to detect trends in the variation of relative search volume (RSV) related to search strategies over time. The most popular queries were analyzed qualitatively, whereas geographical heat maps were retrieved to determine search volumes according to countries (P
- Published
- 2019
29. Orofacial Dysfunction In Cleft And Non-Cleft Patients Using Nordic Orofacial Test - A Screening Study
- Author
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M. N. Sano, Ana Lúcia Pompéia Fraga de Almeida, Natalia Cristina Reinaldo Mariano, Simone Soares, Karin Hermana Neppelenbroek, and Thais Marchini de Oliveira
- Subjects
Adult ,Cleft Lip ,mastication ,Dentistry ,Physical examination ,03 medical and health sciences ,Sex Factors ,0302 clinical medicine ,Swallowing ,Humans ,Medicine ,Craniofacial ,General Dentistry ,Mastication ,Gender comparison ,Screening study ,Aged ,cleft palate ,craniofacial anomalies ,medicine.diagnostic_test ,business.industry ,030206 dentistry ,Middle Aged ,Test (assessment) ,Cleft Palate ,Stomatognathic system ,Face ,orofacial cleft(s) ,DISFUNÇÃO TEMPOROMANDIBULAR ,business ,swallowing ,030217 neurology & neurosurgery - Abstract
Cleft lip, alveolus and palate is a congenital malformation caused by the lack of fusion of the lip and palate embryonic processes, which may disrupt the main functions of the stomatognathic system. Aim: This study aimed to assess the orofacial dysfunction in individuals with cleft lip, alveolus, and palate compared to non-cleft patients. One hundred and twenty individuals between 32 and 65 years of age were selected in the Craniofacial Center and in the School of Dentistry and divided into two groups: non-cleft patients (N-CLAP) and cleft lip, alveolus, and palate patients (CLAP). The two groups were matched by gender. Each individual was interviewed and submitted to a clinical evaluation during which the NOT-S was used to assess orofacial dysfunction. To verify the intra-examiner agreement, the values were compared using the Kappa test. The Mann-Whitney test compared performance on the NOT-S between the groups. The Chi-Square test compared the NOT-S domains between the groups. A comparison of the NOT-S scores between the groups revealed statistically significant differences in gender (p
- Published
- 2019
30. A randomized clinical trial of cavity liners after selective caries removal: one-year follow-up
- Author
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Maria Aparecida de Andrade Moreira Machado, Tássia Carina Stafuzza, Natalino Loureço Neto, Thais Marchini de Oliveira, Thiago Cruvinel, Luciana Lourenço Ribeiro Vitor, Vivien Thiemy Sakai, and Daniela Rios
- Subjects
Molar ,Male ,Time Factors ,medicine.medical_treatment ,Dentistry ,Dental Cements ,law.invention ,030207 dermatology & venereal diseases ,0302 clinical medicine ,Randomized controlled trial ,law ,Dentin ,Radiography, Dental ,Medicine ,Aluminum Compounds ,Child ,Dental Restoration, Permanent ,Oxides ,Primary teeth ,Drug Combinations ,medicine.anatomical_structure ,Treatment Outcome ,Child, Preschool ,Female ,Original Article ,Analysis of variance ,Dental restoration ,Mineral trioxide aggregate ,Dental Caries ,Calcium Hydroxide ,03 medical and health sciences ,Pediatric Dentistry ,Humans ,Tooth, Deciduous ,General Dentistry ,AGREGADO DE TRIÓXIDO MINERAL ,Analysis of Variance ,business.industry ,Silicates ,Dental Cavity Lining ,030206 dentistry ,Calcium Compounds ,lcsh:RK1-715 ,Dental cavity liners ,lcsh:Dentistry ,Dental caries ,Pulp (tooth) ,Zirconium ,Dental Pulp Cavity ,business ,Caries Removal ,Follow-Up Studies - Abstract
Alternatives for the treatment of caries disease, such as minimally invasive approaches, have been developed in recent years. Objective: To carry out clinical and radiographic evaluations of three cavity liners after selective caries removal. Methodology: Thirty-six primary molars with deep occlusal caries lesions without pulp involvement (from children of both genders, aged between 5 and 8 years) were randomly divided into the following groups: calcium hydroxide cement (CHC) group; mineral trioxide aggregate (MTA) group and Portland cement with added zirconium oxide (PCZ) group. The following-up period was 6- and 12-month. The clinical and radiographic success rates were evaluated through chi-square test. The radiographic measurements were compared by ANOVA followed by Tukey's test (p0.05). Nineteen radiographs were selected to measure the dentin barrier thickness. The intragroup comparison presented a statistically significant increase of the dentin barrier for all groups, at 12-month follow-up. However, the MTA group showed increase of the dentin barrier, over time, 6- to 12-month follow-up. The intergroup comparison revealed no statistically significant differences (p>0.05). Conclusion: The clinical and radiographic data showed that all cavity liners provided effective treatment of primary teeth after selective caries removal.
- Published
- 2019
31. Effect of single and multiple doses of low-level laser therapy on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED)
- Author
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Nádia Carolina Teixeira Marques, Vivien Thiemy Sakai, Luciano Aparecido de Almeida-Júnior, Thais Marchini de Oliveira, and M. T. O. Prado
- Subjects
Cell Survival ,medicine.medical_treatment ,Sulforhodamine B ,Cell Count ,Dermatology ,Tooth Exfoliation ,Andrology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Humans ,Viability assay ,Low-Level Light Therapy ,Tooth, Deciduous ,Low level laser therapy ,Cell Proliferation ,Cell growth ,Stem Cells ,Dose-Response Relationship, Radiation ,030206 dentistry ,CÉLULAS ,Dose–response relationship ,chemistry ,Cell culture ,Surgery ,Trypan blue ,Stem cell - Abstract
The present study aimed to evaluate in vitro whether the low-level laser (LLL) delivering fractionated total energy (multiple irradiation) or single irradiation stimulates regeneration-associated events (viability and proliferation) in stem cells from human exfoliated deciduous teeth (SHED). Cells received LLL irradiation (InGaAlP–660 nm), according to the following experimental groups: G1 (single irradiation 2.5 J/cm2, 10 mW, 10 s, 0.10 J), G2 (single irradiation 5.0 J/cm2, 10 mW, 20 s, 0.20 J), G3 (single irradiation 7.5 J/cm2, 10 mW, 30 s, 0.30 J), G4 (two irradiations 2.5 J/cm2, 10 mW, 10 s; total energy 0.20 J), G5 (three irradiations 2.5 J/cm2, 10 mW, 10 s; total energy 0.30 J), and G6 (non-irradiated). Cell viability was assessed by MTT and trypan blue exclusion (TBE) methods, while cell proliferation was evaluated by crystal violet (CV) and sulforhodamine B (SRB) assays after 24, 48, and 72 h after the first irradiation. By MTT, there was no difference between groups at 24 and 72 h. At 48 h, the groups subjected to multiple irradiation (G4 and G5) presented higher cell viability rates. The average percentages of viable cells for all groups by TBE method were 91.04%, 96.63%, and 97.48% at 24, 48, and 72 h, respectively. By CV, there was no significant difference between groups at 24 and 48 h; at 72 h, G2, G3, and G4 presented higher cell proliferation. By SRB, G1 and G4 presented lower proliferation rates in all the periods. When the groups presenting the same total energy were compared, G2 (0.20 J) presented lower cell viability rates and higher cell proliferation rates in comparison with G4; G3 (0.30 J) presented similar results to those of G5, with higher cell viability and proliferation. The application of laser delivering fractionated total energy (two or three applications of 2.5 J/cm2) induced higher cell viability at 48 h, while the single irradiation with 2.5 J/cm2 did not stimulate metabolic activity in such period and the proliferation over time. The 5.0 and 7.5 J/cm2 single doses and the three applications of 2.5 J/cm2 maintained cell viability and stimulated proliferation of SHED at 72 h.
- Published
- 2019
32. Novel rare variations in IRF6 in subjects with non-syndromic cleft lip and palate and dental agenesis
- Author
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Fernanda Veronese Oliveira, Thais Francini Garbieri, Lucimara Teixeira das Neves, Thiago José Dionísio, Viviane A. Parisi, Thais Marchini de Oliveira, and Carlos Ferreira dos Santos
- Subjects
Male ,0301 basic medicine ,Pediatrics ,medicine.medical_specialty ,Candidate gene ,Adolescent ,Cleft Lip ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Missense mutation ,Child ,General Dentistry ,Anodontia ,High prevalence ,Direct sequencing ,business.industry ,Dental agenesis ,030206 dentistry ,medicine.disease ,Cleft Palate ,stomatognathic diseases ,GENÉTICA ,030104 developmental biology ,Otorhinolaryngology ,GENES REGULADORES ,Agenesis ,Interferon Regulatory Factors ,Female ,IRF6 ,business ,Non syndromic - Abstract
Objective Subjects with cleft lip and palate (CLP) present high prevalence of dental agenesis. Among candidate genes for these phenotypes is IRF6. However, genetic studies do not analyze dental agenesis as a phenotype associated with cleft. Therefore, we investigated the frequency of rare and novel variations in IRF6 in subjects with non-syndromic unilateral cleft lip and palate (NSUCLP), with and without dental agenesis. Subjects and methods Genomic DNA samples of 100 subjects with NSUCLP with and without dental agenesis and 50 controls were sequenced. IRF6 mutational screening was conducted by direct sequencing. Results Ten new and rare missense variations were identified, two in the group cleft with agenesis and eight in the group cleft without agenesis, and none were found in control group. In silico analysis revealed four variations as potentially deleterious, being two in the group with cleft and agenesis and two in the group with cleft without agenesis. Conclusion The study identified novel IFR6 variations in subjects with NSUCLP with or without associated dental agenesis. The hypothesis of a higher frequency of deleterious variations in the subjects with cleft associated with dental agenesis, when compared to the group of cleft without agenesis and control without cleft, was not supported.
- Published
- 2019
33. Comparison between calcium hydroxide mixtures and mineral trioxide aggregate in primary teeth pulpotomy: a randomized controlled trial
- Author
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Camila Soares Lopes, Leopoldo Cosme-Silva, Rafael Tobias Moretti Neto, Ana Paula Pereira da Silveira, João Eduardo Gomes-Filho, Thais Marchini de Oliveira, Vivien Thiemy Sakai, Lidiane Lucas Costa e Silva, Ana Beatriz Silveira Moretti, Faculdade de Odontologia, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
Molar ,Mineral trioxide aggregate ,Time Factors ,medicine.medical_treatment ,Radiodensity ,Calcium hydroxide ,Pulpotomy ,Dentistry ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,stomatognathic system ,Radiography, Dental ,medicine ,Humans ,Prospective Studies ,ENSAIO CLÍNICO CONTROLADO RANDOMIZADO ,Tooth, Deciduous ,Aluminum Compounds ,Child ,General Dentistry ,Saline ,business.industry ,Silicates ,Oxides ,030206 dentistry ,Calcium Compounds ,Resorption ,Deciduous tooth ,lcsh:RK1-715 ,Drug Combinations ,stomatognathic diseases ,Treatment Outcome ,chemistry ,lcsh:Dentistry ,Child, Preschool ,Pulp (tooth) ,Original Article ,Saline Solution ,Randomized trial ,business ,Pulp Capping and Pulpectomy Agents ,PULPECTOMIA ,Follow-Up Studies - Abstract
Made available in DSpace on 2019-10-06T16:33:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01. Added 1 bitstream(s) on 2019-10-09T18:35:28Z : No. of bitstreams: 1 S1678-77572019000100450.pdf: 538138 bytes, checksum: d2b685a54cc928f8bc8b772b83b4f774 (MD5) Objectives: To evaluate the effect of calcium hydroxide (CH) associated with two different vehicles as a capping material for pulp tissue in primary molars, compared with mineral trioxide aggregate (MTA). Methodology: Forty-five primary mandibular molars with dental caries were treated by conventional pulpotomy using one of the following materials: MTA only (MTA group), CH with saline (CH+saline group) and CH with polyethylene glycol (CH+PEG group) (15 teeth/group). Clinical and periapical radiographic examinations of the pulpotomized teeth were performed 3, 6, and 12 months after treatment. Data were tested by chi-squared analysis and a multiple comparison post-test. Results: The MTA group showed both clinical and radiographic treatment success in 14/14 teeth (100%), at all follow-up appointments. By clinical evaluation, no teeth in the CH+saline and CH+PEG groups had signs of mobility, fistula, swelling or inflammation of the surrounding gingival tissue. However, in the CH+saline group, radiographic analysis detected internal resorption in up to 9/15 teeth (67%), and inter-radicular bone resorption and furcation radiolucency in up to 5/15 teeth (36%), from 3 to 12 months of follow-up. In the CH+PEG group, 2/11 teeth (18%) had internal resorption and 1/11 teeth (9%) presented bone resorption and furcation radiolucency at all follow-up appointments. Conclusion: CH with PEG performed better than CH with saline as capping material for pulpotomy of primary teeth. However, both combinations yielded clinical and radiographic results inferior to those of MTA alone. Universidade Federal de Alfenas (UNIFAL) Faculdade de Odontologia Departamento de Clínica e Cirurgia Universidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Dentística Restauradora-Endodontia Universidade de São Paulo Faculdade de Odontologia de Bauru Departamento de Odontopediatria Ortodontia e Saúde Coletiva Universidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Dentística Restauradora-Endodontia
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- 2019
34. Orofacial functions and quality of life in children with unilateral cleft lip and palate
- Author
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Taís de Souza Barbosa, Maria Beatriz Duarte Gavião, Ana Bheatriz Marangoni Montes, and Thais Marchini de Oliveira
- Subjects
Male ,Cleft Lip ,Statistics, Nonparametric ,03 medical and health sciences ,Disability Evaluation ,0302 clinical medicine ,Quality of life ,Surveys and Questionnaires ,Humans ,Medicine ,General Materials Science ,030212 general & internal medicine ,Child ,Orthodontics ,Mouth ,business.industry ,Significant difference ,Case-control study ,030206 dentistry ,Deglutition ,lcsh:RK1-715 ,Cleft Palate ,Facial Asymmetry ,Case-Control Studies ,Face ,lcsh:Dentistry ,Social domain ,Quality of Life ,Mastication ,Female ,ASSIMETRIA FACIAL ,business ,Facial symmetry - Abstract
This study aimed to evaluate the orofacial functions and oral health-related quality of life (OHRQoL) of children with unilateral cleft lip and palate (UCLP). This case-control study included patients with UCLP matched by sex and age with controls (children without UCLP), resulting in the inclusion of a total of 108 eight- to ten-year-old children. Orofacial functions and OHRQoL were evaluated using the Nordic Orofacial Test-Screening (NOT-S) and the Child Perceptions Questionnaire (CPQ 8-1 0 ), respectively. Data normality was assessed by the Kolmogorov-Smirnov test. Differences and correlations in NOT-S and CPQ 8-1 0 scores between and within the groups were evaluated using Mann-Whitney and Spearman´s correlation tests, respectively. The distribution of NOT-S and global ratings of CPQ 8-1 0 for each group were assessed by Chi-squared/Fisher’s Exact tests. The UCLP group had a higher NOT-S total and examination scores than the controls. Dysfunctions related to breathing, facial symmetry/expression, and speech were more frequent in the UCLP patients than in the controls. The UCLP group had higher scores on the social well-being domain than the controls. There was a significant difference between the groups in their ratings in regards to the extent to which their oral condition affected their life overall, with controls perceiving it as somewhat better than patients. In both groups, NOT-S total and interview scores were positively correlated with CPQ 8-1 0 total and domain scores. The NOT-S examination score was only significantly correlated with social domain scores in the control group. The presence of UCLP was associated with clinical signs of orofacial dysfunctions related to breathing, facial symmetry/expression, and speech. Children with UCLP reported more orofacial dysfunctions and negative impacts on social well-being than controls.
- Published
- 2019
35. Digital behavior surveillance: monitoring dental caries and toothache interests of Google users from developing countries
- Author
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Daniela Rios, Thais Marchini de Oliveira, Matheus Lotto, Thiago Cruvinel, Agnes Fátima Pereira Cruvinel, and Patricia Estefania Ayala Aguirre
- Subjects
AMÉRICA DO SUL ,Information Seeking Behavior ,Developing country ,Dental Caries ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Environmental health ,Toothache ,eHealth ,Medicine ,Humans ,Surveillance monitoring ,General Dentistry ,Developing Countries ,Permanent teeth ,Internet ,business.industry ,030206 dentistry ,South America ,stomatognathic diseases ,First trimester ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,South american ,Internet penetration ,medicine.symptom ,business ,Forecasting - Abstract
Objective To determine the differences between the levels of interest of South American Google users on information related to dental caries and toothache over time. Materials and methods The monthly variation of Relative Search Volume (RSV) for both queries was obtained in Google Trends between 2004 and 2017. The association between RSVs, Internet penetration, and disability-adjusted life-years (DALYs) for dental caries in permanent teeth were tested by distinct regression models. Forecasting ARIMA models were developed to analyze trends and predict the interests until December 2018. Results In general, toothache-related searches presented an uptrend associated positively with Internet penetration and negatively with DALYs, whereas dental caries-related searches presented a downtrend associated negatively with Internet penetration and positively with DALYs. The seasonality affected only dental caries curves, with the highest rates observed in the first trimester and the lowest rates in June, July, and December. Conclusion The levels of interests of South American Google users in seeking dental caries and toothache information appear to have a direct connection with the burden of untreated dental caries lesions and Internet penetration. The no association between dental caries- and toothache-related interests reinforces the unfamiliarity of most people about the relationship of dental pain with the final stages of chronic oral diseases.
- Published
- 2019
36. Influence of mandibular and palatal intraoral appliances on erosion in situ study outcome
- Author
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Fernanda Lyrio Mendonça, Maisa Camillo Jordão, N. M. Santos, Daniela Rios, Thais Marchini de Oliveira, B. T. P. Bergantin, Franciny Querobim Ionta, and Heitor Marques Honório
- Subjects
Adult ,Male ,Time Factors ,Adolescent ,Surface Properties ,PROTOCOLOS CLÍNICOS ,Treatment outcome ,Mandible ,Oral cavity ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Orthodontic Appliances ,stomatognathic system ,Hardness ,Surveys and Questionnaires ,Animals ,Humans ,Medicine ,Single-Blind Method ,Tooth Erosion ,Bovine enamel ,Dental Enamel ,Saliva ,General Dentistry ,Orthodontics ,Enamel paint ,Palate ,business.industry ,Equipment Design ,030206 dentistry ,lcsh:RK1-715 ,stomatognathic diseases ,Treatment Outcome ,Patient Satisfaction ,Enamel ,lcsh:Dentistry ,visual_art ,Intraoral appliance ,visual_art.visual_art_medium ,Cattle ,Female ,Original Article ,Dental erosion ,business ,Protocols ,In situ study - Abstract
The standardization of in situ protocols for dental erosion is important to enable comparison between studies. Objective: Thus, the objectives of this study were to evaluate the influence of the location of in situ intraoral appliance (mandibular X palatal) on the extent of enamel loss induced by erosive challenges and to evaluate the comfort of the appliances. Material and Methods: One hundred and sixty bovine enamel blocks were selected according to their initial surface hardness and randomly divided into two groups: GI - palatal appliance and GII - mandibular appliance. Twenty volunteers wore simultaneously one palatal appliance (containing 4 enamel blocks) and two mandibular appliances (each one containing 2 enamel blocks). Four times per day during 5 days, the volunteers immersed their appliances in 0.01 M hydrochloric acid for 2 minutes, washed and reinserted them into the oral cavity for 2 hours until the next erosive challenge. After the end of the in situ phase, the volunteers answered a questionnaire regarding the comfort of the appliances. The loss of tissue in the enamel blocks was determined profilometrically. Data were statistically analyzed by paired t-test, Chi-square and Fisher's Exact Test (p
- Published
- 2019
37. Immediate and mediate furcal perforation treatment in primary molars: 24-month follow-up
- Author
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Nádia Carolina Teixeira Marques, Thais Marchini de Oliveira, and N. Lourenço Neto
- Subjects
Male ,0301 basic medicine ,Molar ,Fistula ,Glass ionomer cement ,Dentistry ,Sodium Chloride ,0302 clinical medicine ,Osteogenesis ,Medicine ,Tooth Root ,Aluminum Compounds ,Child ,Oxides ,Root Canal Therapy ,Drug Combinations ,Mineral trioxide aggregate ,medicine.medical_specialty ,Radiodensity ,Perforation (oil well) ,engineering.material ,Root Canal Filling Materials ,03 medical and health sciences ,stomatognathic system ,Humans ,Dentistry (miscellaneous) ,Tooth, Deciduous ,Dental Pulp ,Wound Healing ,Root Canal Irrigants ,business.industry ,Periapical Diseases ,Silicates ,030206 dentistry ,Calcium Compounds ,medicine.disease ,Surgery ,Amalgam (dentistry) ,stomatognathic diseases ,030104 developmental biology ,Glass Ionomer Cements ,Pulpotomy ,Pediatrics, Perinatology and Child Health ,engineering ,Pulp (tooth) ,DENTE ,Dental Pulp Cavity ,business ,Follow-Up Studies - Abstract
Furcal perforation consists of a communication between the pulp cavity and the periodontal tissues that can occur accidentally during cavity preparation or root canal instrumentation. This complication may lead to inflammatory reactions and compromise adjacent tissues irreversibly, resulting in the early loss of primary teeth. Perforation sealing with a biocompatible material is necessary to ensure a favourable prognosis. Among different materials, MTA has been employed due to its excellent healing and physical properties. The purpose of these case reports was to describe immediate and mediate cases of furcal perforation treatment using MTA in primary molars. In both the cases, after accidental perforation detection, the pulp chamber was cleaned with saline solution to clear off the debris. The wound surface was continuously irrigated with saline solution and a dry sterile cotton pellet was placed on the radicular pulp stumps and perforation area with slight pressure until the bleeding was controlled. The MTA was applied into the perforation and pulp chamber area with the aid of a sterile amalgam carrier. The teeth were restored with resin-modified glass ionomer cement. Clinical and radiographic follow-up examinations were carried out at 3, 6, 12, 18, and 24 months after perforation sealing. Both the cases remained asymptomatic, presenting no discomfort, mobility, swelling, or fistula after 24-months follow-up. Radiographically, the radiolucent area disappeared as a result of bone formation in the inter-radicular space, showing adjacent tissue preservation. Thus, MTA may be considered as an ideal option for conservative treatment of immediate and mediate furcal perforation in primary teeth, once it promoted repair with tooth maintenance in both the cases.
- Published
- 2016
38. Comparison of two treatment protocols in children with unilateral complete cleft lip and palate: Tridimensional evaluation of the maxillary dental arch
- Author
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Thais Marchini de Oliveira, Wanda Gnoinski, Karine Vaz Laskos, Daniela Gamba Garib, Maria Aparecida de Andrade Moreira Machado, Cleide Felício de Carvalho Carrara, Terumi Okada Ozawa, Fabrício Pinelli Valarelli, and Paula Karine Jorge
- Subjects
Male ,medicine.medical_specialty ,Cleft Lip ,Orthopedic plate ,Group ii ,Dentistry ,03 medical and health sciences ,Dental Arch ,0302 clinical medicine ,Humans ,Medicine ,Orthopedic Procedures ,Arch ,030223 otorhinolaryngology ,Orthodontics ,Maxillary arch ,Arch form ,business.industry ,Infant ,030206 dentistry ,Models, Dental ,Unilateral complete cleft lip ,Cleft Palate ,Dental arch ,Treatment Outcome ,medicine.anatomical_structure ,Otorhinolaryngology ,Orthopedic surgery ,Female ,Surgery ,Oral Surgery ,business ,CEFALOMETRIA - Abstract
Objective The aim of this study was to evaluate the dimensional alterations of the maxillary dental arches after lip repair in children with unilateral complete cleft lip and palate of two different protocols. Material and methods The sample was composed of 94 digital models and divided in two groups: Group I – 23 children, (protocol 1) – with presurgical orthopedic intervention: Hotz plate; Group II – 24 children, (protocol 2) – without presurgical orthopedic intervention. The three dimensional images of the maxillary arch were obtained before lip repair (stage 1) and at approximately 1 year of age (stage 2). Interstages changes for each group were evaluated using dependent t test and Wilcoxon test, and in the intergroup were used independent t test, and Mann–Whitney test. Results The comparison of the dimensional alterations of dental arches between the two protocols exhibited differences: in the stage 1, the intercanine was smaller in Group II; in the stage 2, the anterior cleft width, the intercanine and the anteroposterior cleft were smaller in Group II. Conclusion The results reported here suggest that the use of infant orthopedic plate as applied in protocol 1 may favor correct arch form establishment in infants with unilateral complete cleft lip and palate.
- Published
- 2016
39. General anesthesia for dental care management of a patient with epidermolysis bullosa: 24-month follow-up
- Author
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Natalino Lourenço Neto, Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Tatiana Yuriko Kobayashi, Eloá Cristina Passucci Ambrosio, Bianca Zeponi Fernandes Mello, Marina Barbosa Almeida Mello, and Renato Yassutaka Faria Yaedú
- Subjects
medicine.medical_specialty ,Permanent tooth ,integumentary system ,Dental Care for Chronically Ill ,business.industry ,Dental Care for Children ,Mucocutaneous zone ,030206 dentistry ,medicine.disease ,Dental care ,Dermatology ,Oral hygiene ,Surgery ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Anesthesia ,medicine ,Epidermolysis bullosa ,business ,General Dentistry ,Month follow up - Abstract
Epidermolysis bullosa comprises a group of uncommon skin-related diseases, characterized by the formation of blisters on mucocutaneous regions occurring spontaneously, following a trauma, exposure to heat, or as a result of minimal mechanical trauma. The dental treatment of the patient with epidermolysis bullosa raises many questions and discussions, due to the difficulty of carrying out the procedures. This report aimed to detail the clinical considerations of the treatment under general anesthesia of a patient with epidermolysis bullosa. The extraction of all deciduous teeth under general anesthesia was recommended based on the clinical and radiographic examinations. At 24-month follow-up, the patient had great improvement in oral hygiene without new caries lesions. The patient has been followed-up at every month for caries lesion prevention and permanent tooth development. The treatment under general anesthesia provided the ideal safe conditions and was beneficial for the patient.
- Published
- 2016
40. Impact of Saliva and Intraoral Appliance on Erosion Lesions Rehardening Ability -A Pilot Study
- Author
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Heitor Marques Honório, Fernanda Lyrio Mendonça, Daniela Rios, Priscilla Santana Pinto Gonçalves, Catarina Ribeiro Barros de Alencar, Thais Marchini de Oliveira, Franciny Querobim Ionta, and Gabriela Cristina de Oliveira
- Subjects
Saliva ,Enamel paint ,business.industry ,Oral appliance ,0206 medical engineering ,Dentistry ,030206 dentistry ,02 engineering and technology ,020601 biomedical engineering ,03 medical and health sciences ,0302 clinical medicine ,visual_art ,Pediatrics, Perinatology and Child Health ,Intraoral appliance ,visual_art.visual_art_medium ,Medicine ,Analysis of variance ,Single blind ,business ,General Dentistry ,In situ study - Abstract
Objective: To evaluate the ability of different periods of salivary exposure and two different removable appliances to rehardening initial erosive lesions. Material and Methods: This randomized, single blind in situ study was conducted with 2 crossover phases. The factors under study were: period of salivary exposure (15 minutes, 30 minutes, 1 hour and 2 hours) and type of oral appliance (maxillary or mandibular). Two hundred enamel blocks were selected by initial surface hardness (SHi). Enamel blocks were demineralized in vitro (0.05M citric acid; pH2.5 for 15 seconds), surface hardness (SHd) was remeasured and 160 blocks were selected and randomized among groups. Thus, there were 2 blocks per period of salivary exposure in each type of oral appliance for each one of the 10 volunteers. In each phase, one of the removable appliances was tested. The response variable was percentage of surface hardness recovery (%SHR=[(SHf-SHd)/SHi)]x100). Two-way ANOVA and Tukey’s post hoc test were applied adopting 5% of significance. Results: No difference was found among oral appliances on enamel rehardening (p>0.01). Salivary exposure of 2 hours promoted similar enamel rehardening when compared to 1 hour (p>0.05), which showed similar rehardening to 30 min. All mentioned period of salivary exposure promoted superior rehardening than 15 min (p>0.01). Conclusion: The salivary time exposure between erosive attacks might be 2 hours to achieve a feasible maximum rehardening. In addition, both maxillary and the mandibular appliance have presented a similar rehardening ability.
- Published
- 2016
41. Using Augmented Reality to Motivate Oral Hygiene Practice in Children: Protocol for the Development of a Serious Game
- Author
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Alexandre Alberto Pascotto Montilha, Susy Nazaré Silva Ribeiro Amantini, Bianca Caseiro Antonelli, Daniela Rios, Natalino Lourenço Neto, Maria Aparecida de Andrade Moreira Machado, Thais Marchini de Oliveira, Kim Tanabe De Moura Leite, and Thiago Cruvinel
- Subjects
Emerging technologies ,Computer science ,Interface (Java) ,computer.software_genre ,Outcome (game theory) ,Oral hygiene ,03 medical and health sciences ,0302 clinical medicine ,Protocol ,computer simulation ,030212 general & internal medicine ,Protocol (science) ,Medical education ,business.industry ,ComputingMilieux_PERSONALCOMPUTING ,video games ,education, dental ,030206 dentistry ,General Medicine ,pediatric dentistry ,Action (philosophy) ,user-computer interface ,Virtual machine ,ODONTOPEDIATRIA ,Augmented reality ,business ,computer - Abstract
Background New technologies create possible new ways of action, interaction, and learning which is extremely relevant in the field of oral health education. There is a lack of protocol in using an immersive interactive ludic-educational interface to motivate oral hygiene practice in children by means of augmented reality. Objective This study aims to present a protocol on the development of a serious game to motivate oral hygiene practice in children. Methods A serious game will be designed by augmented reality techniques to improve toothbrushing effectiveness of children aged 6 to 10 years. The functional structure of this interface is activated by means of movements recognized by Kinect (Microsoft Corp). The toothbrushing technique will be available in the game, enabling the children to execute the movement in the virtual environment. By identifying errors, this game will be tailored to improve the oral health of children by correcting the technique and teaching the user the adequate toothbrushing method. A template analysis will be performed to identify barriers and facilitators in each scenario. Results After the implementation of the virtual interactive and immersive panels, enrollment will begin and evaluations will be made by means of questionnaires distributed to participants who interact with the game. Thus, an analysis of the product efficacy will be conducted. The expected outcome will be to obtain a digital instrument to motivate oral hygiene practice and enhance health awareness in children. Conclusions The serious game will support the prevention of oral diseases by sharing scientific research in the school environment and community. International Registered Report Identifier (IRRID) PRR1-10.2196/10987
- Published
- 2020
42. Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?
- Author
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L. L. R. Vitor, M. T. O. Prado, Thiago José Dionísio, Thiago Cruvinel, Maria Aparecida de Andrade Moreira Machado, Vivien Thiemy Sakai, Carlos Ferreira dos Santos, Daniela Rios, Natalino Lourenço Neto, Thais Marchini de Oliveira, and Rodrigo Cardoso de Oliveira
- Subjects
Vascular Endothelial Growth Factor A ,animal structures ,Lysis ,Cell Survival ,030303 biophysics ,Cell ,Biophysics ,02 engineering and technology ,Flow cytometry ,03 medical and health sciences ,medicine ,Humans ,Cell Lineage ,Radiology, Nuclear Medicine and imaging ,Secretion ,Viability assay ,Tooth, Deciduous ,Cells, Cultured ,Cell Proliferation ,0303 health sciences ,Vascular Endothelial Growth Factor Receptor-1 ,Radiation ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,Chemistry ,Lasers ,Stem Cells ,DENTE DECÍDUO ,Fibroblasts ,Cell cycle ,021001 nanoscience & nanotechnology ,Vascular Endothelial Growth Factor Receptor-2 ,Molecular biology ,medicine.anatomical_structure ,Pulp (tooth) ,Stem cell ,0210 nano-technology - Abstract
This study aimed to compare the synthesis and secretion of VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, and FGF-2 between pulp fibroblasts from human primary teeth (HPF) and stem cell from human deciduous teeth (SHED) before and after photobiomodulation. HPF were obtained from explant technique and characterized by immunohistochemistry, while SHED were obtained from digestion technique and characterized by flow cytometry. HPF (control group) and SHED were plated, let to adhere, and put on serum starvation to synchronize the cell cycles prior to photobiomodulation. Then, both cell lineages were irradiated with 660-nm laser according to the following groups: 2.5 and 3.7 J/cm2. MTT and crystal violet assays respectively verified viability and proliferation. ELISA Multiplex Assay assessed the following proteins: VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, FGF-2, at 6, 12, and 24 h after photobiomodulation, in supernatant and lysate. Two-way ANOVA/Tukey test evaluated cell viability and proliferation, while angiogenic production and secretion values were analyzed by one-way ANOVA (P .05). HPF exhibited statistically greater values of all angiogenic proteins than did SHED, at all study periods, except for FGF-2 (supernatant; 12 h); VEGFR1 (lysate; non-irradiated; 12 h); and VEGFR1 (lysate; non-irradiated; 24 h). Photobiomodulation changed the synthesis and secretion of angiogenic proteins by HPF. HPF produced and secreted greater values of all tested angiogenic proteins than did SHED before and after irradiation with both energy densities of 2.5 and 3.7 J/cm2.
- Published
- 2020
43. Is there a relationship of negative oral health beliefs with dental fear and anxiety regarding diverse dental patient groups? A systematic review and meta-analysis
- Author
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Anna Paola Strieder, Thais Marchini de Oliveira, Agnes Fátima Pereira Cruvinel, Daniela Rios, and Thiago Cruvinel
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Adolescent ,Culture ,Scopus ,Dental fear ,Oral Health ,Oral health ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Dental Anxiety ,Medicine ,Humans ,Child ,Dental Care ,General Dentistry ,business.industry ,METANÁLISE ,030206 dentistry ,medicine.disease ,Clinical trial ,stomatognathic diseases ,Cross-Sectional Studies ,030220 oncology & carcinogenesis ,Meta-analysis ,Case-Control Studies ,Cohort ,Anxiety ,Observational study ,medicine.symptom ,business ,Clinical psychology - Abstract
This systematic review and meta-analysis aimed to critically appraise the evidence on the relationship of oral health beliefs with dental fear and anxiety in distinct patient groups. Observational studies were retrieved by Cochrane, Embase Search, Portal BVS, Clinical Trials, Ovid, Open Gray, PubMed, Scopus, and Web of Science, and they were manually checked for the inclusion of additional articles of interest. The assessment of quality of studies was performed by the application of three different versions of the Newcastle-Ottawa Scale, for cohort, case-control, and cross-sectional studies. Only studies with low or unclear/moderate risk of bias contributed to meta-analyses, regarding the analysis of random effects of mean differences of dental beliefs scores between dental fear/anxiety and control groups, and the correlation of dental beliefs with dental fear and anxiety measures. Of 276 articles initially retrieved, 10 were included in the systematic review, while only 6 studies with unclear/moderate risk of bias were considered in meta-analyses. The mean difference of dental beliefs effects was higher in patients with dental fear and anxiety compared to controls (1.20; 95% CI 0.27–2.14; P = 0.01). Additionally, a moderate positive correlation was observed between dental beliefs and dental fear measures (r = 0.54, 95% CI 0.47–0.60;P
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- 2018
44. In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel
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Annelyze Podolan Kloster, Maria Aparecida de Andrade Moreira Machado, Natalino Lourenço Neto, Thais Marchini de Oliveira, Rodrigo Cardoso de Oliveira, and Silgia Aparecida da Costa
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CLOREXIDINA ,Bioadhesive ,Microbial Sensitivity Tests ,02 engineering and technology ,macromolecular substances ,In Vitro Techniques ,Bacterial Adhesion ,Streptococcus mutans ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Antimicrobial effect ,Candida albicans ,medicine ,Humans ,Food science ,General Dentistry ,biology ,Chlorhexidine ,chlorhexidine ,Mouth Mucosa ,technology, industry, and agriculture ,030206 dentistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,In vitro ,Membrane ,chemistry ,Anti-Infective Agents, Local ,anti-infective agents ,Layer-by-layer coatings ,chitosan ,0210 nano-technology ,Gels ,medicine.drug - Abstract
This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues. Five oral membranes of different compositions were tested: 5% chitosan (G1); 5% chitosan ± 0.2% chlorhexidine (G2), 5% chitosan ± 0.6% chlorhexidine (G3), 5% chitosan ± 1.0% chlorhexidine (G4), and 5% chitosan ± 2.0% chlorhexidine (G5). Also, five gel types were tested according to the following compositions: 5% chitosan gel (G6), 0.2% chlorhexidine gel (G7), 2.0% chlorhexidine gel (G8), 5% chitosan gel ± 0.2% chlorhexidine gel (G9), and 5% chitosan gel ± 2.0% chlorhexidine gel (G10). The antimicrobial action of the samples was tested against Candida albicans and Streptococcus mutans through antibiogram by measuring the inhibition halos. Data were statistically analyzed by Kruskal-Wallis and one-way ANOVA followed by Tukey test (p
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- 2018
45. The Brazilian validation of a health literacy instrument: the newest vital sign
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Daniela Alejandra Cusicanqui Méndez, Agnes Fátima Pereira Cruvinel, Thiago Cruvinel, Matheus Lotto, G. C. Chaves, Thais Marchini de Oliveira, and E. Gutierres
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Newest vital sign ,Adult ,Male ,Medical education ,Psychometrics ,Reproducibility of Results ,Health literacy ,Oral Health ,030206 dentistry ,General Medicine ,Oral health ,Middle Aged ,Health Literacy ,SAÚDE PÚBLICA ,03 medical and health sciences ,0302 clinical medicine ,Surveys and Questionnaires ,Humans ,Female ,030212 general & internal medicine ,Adaptation (computer science) ,Psychology ,Dental Health Surveys ,General Dentistry ,Brazil - Abstract
This study aimed to perform the cross-cultural adaptation and validation of the NVS for Brazilian Portuguese.Two hundred and fifty adults responded to the adapted version of the NVS, the Brazilian version of the Rapid Estimate of Adult Literacy in Dentistry (BREALD-30), ten questions of the National Functional Literacy Index (NFLI), and a questionnaire about demographic and oral health-related aspects. Statistical analysis determined the internal consistency, stability, difficulty of items, and convergent/discriminant/predictive validities of the NVS. p .05 was considered significant.The NVS displayed a good internal consistency (Cronbach's alpha = 0.79) and a fair stability (ICC = 0.57; 0.39-0.70 95% CI). Seventy-two percent of participants answered the easier question (#5) correctly, whereas only 28.4% were successful in responding the most difficult question (#1). The convergent validity of the NVS was demonstrated by its significant correlation with the BREALD-30 (Rs = 0.601, p .001) and NFLI (Rs = 0.544, p .001). The observation of higher NVS scores among health professionals, white and more educated people confirmed the discriminant validity of the instrument. Moreover, health literacy was a significant predictor of self-reported oral health and reason for dental utilization.The NVS demonstrated adequate psychometric properties to be applied in Brazilian oral health epidemiological surveys.
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- 2018
46. Impact of a simplified in situ protocol on enamel loss after erosive challenge
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Maisa Camillo Jordão, Thais Marchini de Oliveira, Marília Afonso Rabelo Buzalaf, Heitor Marques Honório, Daniela Rios, N. M. Santos, Fernanda Lyrio Mendonça, Thiago Cruvinel, Camilla Cristina Lira Di Leone, and Franciny Querobim Ionta
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Male ,Teeth ,Physiology ,lcsh:Medicine ,Marine and Aquatic Sciences ,Chi Square Tests ,Salivary Glands ,Fluorides ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Surveys and Questionnaires ,Medicine and Health Sciences ,Maxilla ,Medicine ,Single-Blind Method ,Tooth Erosion ,lcsh:Science ,Flow Rate ,Orthodontics ,Multidisciplinary ,Cross-Over Studies ,Enamel paint ,Physics ,Classical Mechanics ,Body Fluids ,Chemistry ,030220 oncology & carcinogenesis ,visual_art ,Physical Sciences ,visual_art.visual_art_medium ,Female ,Anatomy ,Statistics (Mathematics) ,Research Article ,Freshwater Environments ,Adult ,Surface Properties ,Fluid Mechanics ,Research and Analysis Methods ,Continuum Mechanics ,APARELHOS ORTODÔNTICOS ,03 medical and health sciences ,Young Adult ,Exocrine Glands ,stomatognathic system ,Tongue ,Orthodontic Appliances ,Hardness ,Humans ,Bovine enamel ,Statistical Methods ,Saliva ,Dental Enamel ,Statistical Hypothesis Testing ,Parotid Glands ,Mouth ,business.industry ,lcsh:R ,Ecology and Environmental Sciences ,Chemical Compounds ,Biology and Life Sciences ,Aquatic Environments ,Fluid Dynamics ,030206 dentistry ,Bodies of Water ,Crossover study ,Surface loss ,stomatognathic diseases ,Lakes ,Jaw ,Tooth Remineralization ,Earth Sciences ,lcsh:Q ,Single blind ,business ,Digestive System ,Head ,Mathematics ,In situ study - Abstract
This study investigated the effect of the period of use and location of intraoral appliances on enamel surface loss. This randomized, single blind in situ study was conducted in 2 crossover phases based on the period of use, in which maxillary and mandibular appliances were simultaneously worn. Bovine enamel blocks (n = 120) were randomly divided among the studied groups by surface hardness. In each phase, fifteen volunteers used one maxillary appliance and two mandibular appliances for 5 days. Erosive challenge was performed 4X/day by immersion in 0.01 M HCL for 2 minutes. In the continuous phase, the intraoral appliances were worn for 20 hours. In the intermittent phase the appliances were worn for 8 hours and 30 minutes. Enamel loss was determined profilometrically. The discomfort of use of the appliances were evaluated in a questionnaire. Data were analyzed by two-way ANOVA/Tukey's test and chi-square test (p
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- 2018
47. Evaluation of Dentin-Pulp Complex Response after Conservative Clinical Procedures in Primary Teeth
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Daniela Rios, Tássia Carina Stafuzza, Thiago Cruvinel da Silva, Bianca Zeponi Fernandes Mello, Luciana Lourenço Ribeiro Vitor, Thais Marchini de Oliveira, and Maria Aparecida de Andrade Moreira Machado
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Molar ,Radiography ,Dentistry ,Orthodontics ,Dental pulp capping ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,CAPEAMENTO DA POLPA DENTÁRIA ,Dentin ,medicine ,Deciduous teeth ,030219 obstetrics & reproductive medicine ,business.industry ,Deciduous ,030206 dentistry ,Exact test ,Tooth ,medicine.anatomical_structure ,Clinical research ,Pediatrics, Perinatology and Child Health ,Dental caries ,Periodontics ,Pulp (tooth) ,Original Article ,Oral Surgery ,business ,Caries Removal - Abstract
Introduction Although selective caries tissue removal decreases the number and diversity of bacteria, stops the caries process, and reduces the risk of pulp exposure, the studies on the minimally removal of caries tissue are limited and further clinical research is necessary in this field. Aim This study aimed to evaluate through clinical and radiographic assessments the in vivo response of the dentin-pulp complex of human deciduous teeth after either partial or total caries removal (TCR). Materials and methods A total of 49 deciduous molars of children aged between 5 and 9 years were carefully selected. The teeth were divided into two groups: Group I: Partial removal of caries; group II: Total removal of caries. Clinical and radiographic evaluations were performed during the period of 4 to 6 months after the procedure. The intraexam-iner reproducibility was determined by Kappa test. Fisher's exact test was used to determine the statistical difference between groups. Results All teeth showed clinical success during the 4- to 6-month evaluation period. The radiographic evaluation showed 94.2 and 89.6% of success rate in groups I and II respectively. Radiographic results did not show statistically significant differences between the studied groups (p > 0.05). Conclusion The partial caries removal (PCR) showed satisfactory clinical and radiographic outcomes, suggesting that this minimally invasive approach might replace the TCR when correctly indicated.How to cite this article: Mello B, Stafuzza TC, Vitor L, Rios D, Silva T, Machado M, Oliveira TM. Evaluation of Dentin-Pulp Complex Response after Conservative Clinical Procedures in Primary Teeth. Int J Clin Pediatr Dent 2018;11(3):188-192.
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- 2018
48. Impact of Orofacial Dysfunction on the Quality of Life of Adult Patients With Cleft Lip and Palate
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Victor Prado Curvêllo, M. N. Sano, Karin Hermana Neppelenbroek, Ana Lúcia Pompéia Fraga de Almeida, Natalia Cristina Reinaldo Mariano, Simone Soares, and Thais Marchini de Oliveira
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medicine.medical_specialty ,Pediatrics ,Adult patients ,business.industry ,030206 dentistry ,03 medical and health sciences ,0302 clinical medicine ,Otorhinolaryngology ,Quality of life ,Epidemiology ,medicine ,030212 general & internal medicine ,Oral Surgery ,Craniofacial ,business - Abstract
Objective: This study assessed the prevalence of orofacial dysfunctions (ODs) and quality of life (QoL) in adults with and without a cleft lip and palate. Design: Cross sectional. Setting: Craniofacial Center, Hospital for Rehabilitation of Craniofacial Anomalies, Bauru, São Paulo, Brazil. Methods: The study was composed of a sample of 120 patients: 60 adults in the cleft lip and palate group (CLPG) and 60 adults in the control group with no craniofacial anomalies. Each patient underwent an interview and clinical examination, using the Nordic Orofacial Test-Screening (NOT-S) and the 36-Item Short Form Survey. Data were analyzed using Mann-Whitney U test, χ2, and the Spearman correlation coefficients. Results: There was a higher prevalence of OD in CLPG ( P < .001) on the NOT-S. The adults in the CLPG had higher QoL in the areas of general health ( P = .003), physical function ( P = .014), social function ( P < .001), and vitality ( P = .006). The CLPG had significant associations between higher OD and lower QoL for general health ( P = .004), emotional role function ( P = .028), and vitality ( P = .05). Conclusion: Orofacial dysfunctions were more prevalent in adults with a cleft, negatively impacting their QoL in general health, emotional role function, and vitality. However, adults with a cleft also had significantly higher QoL, reflecting possible resiliency when compared to adults without a cleft.
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- 2018
49. Oral health-related quality of life of children with oral clefts and their families
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Maria Beatriz Duarte Gavião, Daniela Rios, G. M. Rando, Luciana Lourenço Ribeiro Vitor, Simone Soares, Thiago Cruvinel da Silva, Maria Aparecida de Andrade Moreira Machado, Cleide Felício de Carvalho Carrara, Paula Karine Jorge, and Thais Marchini de Oliveira
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Male ,Quality of life ,Questionnaires ,Activities of daily living ,Oral health ,MEDLINE ,Dentistry ,Group comparison ,Statistics, Nonparametric ,03 medical and health sciences ,0302 clinical medicine ,Sex Factors ,Statistical significance ,Sickness Impact Profile ,Surveys and Questionnaires ,Medicine ,Humans ,Family ,030212 general & internal medicine ,Early childhood ,Child ,General Dentistry ,business.industry ,Cleft lip ,Case-control study ,Age Factors ,030206 dentistry ,Original Articles ,humanities ,lcsh:RK1-715 ,stomatognathic diseases ,QUESTIONÁRIOS ,Cleft palate ,Case-Control Studies ,Child, Preschool ,lcsh:Dentistry ,Female ,business - Abstract
Oral health problems can influence people's Quality of Life (QoL) because of pain, discomfort, limitations, and other esthetics problems, affecting their social life, feeding, daily activities, and the individual's well-being. Objective: To compare oral health-related quality of life (OHRQoL) of children with and without oral clefts and their families. Materials and Methods: 121 children aged from 2 to 6 years, from both sexes, enrolled in the treatment routine of the Pediatric Dentistry Clinics of a Dental School and a Hospital for Cleft Treatment were divided into two groups: Group 1 - children with cleft lip and palate; Group 2 - children without cleft lip and palate. The OHRQoL was assessed using the validated Portuguese version of the Early Childhood Oral Health Impact Scale (B-ECOHIS). The questionnaire was answered individually, only once, at a private place. Mann-Whitney U test was used to verify differences between groups. Spearman's Rho test was used to associate sex and age with quality of life. The level of significance was set at 5% (p
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- 2018
50. A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED
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Cristiane Duque, Nelson Pereira Marques, Leopoldo Cosme-Silva, João Adolfo Costa Hanemann, Camila Soares Lopes, Vivien Thiemy Sakai, Thais Marchini de Oliveira, Nádia Carolina Teixeira Marques, Fed Univ Alfenas UNIFAL MG, Jose do Rosario Vellano Univ UNIFENAS, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
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Red laser ,Cell Survival ,Infrared Rays ,Stem cells ,Dermatology ,LASER NÃO CIRÚRGICO ,Tooth Exfoliation ,Cell survival ,law.invention ,Andrology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Laser therapy ,law ,Humans ,Irradiation ,Viability assay ,Tooth, Deciduous ,Cell proliferation ,Cell Proliferation ,Chemistry ,Cell growth ,Proliferative capacity ,Lasers ,Stem Cells ,Far-infrared laser ,030206 dentistry ,Laser ,Low-level light therapy ,Surgery ,Stem cell - Abstract
Made available in DSpace on 2019-10-04T12:36:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2J/cm(2) (0.05J), 2.5J/cm(2) (0.1J), 5.0J/cm(2) (0.2J), and 7.5J/cm(2) (0.3J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P
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- 2018
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