Search

Your search keyword '"Weiling Maggie, Cai"' showing total 2 results
Start Over Author "Weiling Maggie, Cai" Topic 03 medical and health sciences
2 results on '"Weiling Maggie, Cai"'

1. Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA

Author

Weiling Maggie Cai, Jin Wang, Zhenguo Lin, Liang Cui, Hongping Dong, Luang Xu, Samie R. Jaffrey, Timothy K. Lu, Seetharamsingh Balamkundu, Dahai Luo, Peter C. Dedon, Chuan-Fa Liu, Bing Liang Alvin Chew, Yong Lai, Pei Yong Shi, Xin-Yuan Fu, School of Biological Sciences, Interdisciplinary Graduate School (IGS), Lee Kong Chian School of Medicine (LKCMedicine), and NTU Institute of Health Technologies

Subjects
RNA Caps, Sequence analysis, Mass Spectrometry-based Technique, Metabolite, Saccharomyces cerevisiae, Biology, Dengue virus, medicine.disease_cause, RNA Characterisation and Manipulation, Epigenesis, Genetic, Transcriptome, 03 medical and health sciences, Mice, chemistry.chemical_compound, Genetics, medicine, Animals, Humans, Medicine [Science], Nucleotide, RNA Processing, Post-Transcriptional, Escherichia coli, Cells, Cultured, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Sequence Analysis, RNA, 030302 biochemistry & molecular biology, Eukaryotic transcription, RNA, Methylation, Dengue Virus, Yeast, Mice, Inbred C57BL, carbohydrates (lipids), chemistry, Biochemistry, Methods Online, RNA, Viral, Female
Abstract

Chemical modification of transcripts with 5’ caps occurs in all organisms. Here we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of the cap epitranscriptome in any organism. The method was piloted with 21 canonical caps – m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG – and 5 “metabolite” caps – NAD, FAD, UDP-Glc, UDP-GlcNAc, and dpCoA. Applying CapQuant to RNA from purified dengue virus,Escherichia coli, yeast, mice, and humans, we discovered four new cap structures in humans and mice (FAD, UDP-Glc, UDP-GlcNAc, and m7Gpppm6A), cell- and tissue-specific variations in cap methylation, and surprisingly high proportions of caps lacking 2’-O-methylation, such as m7Gpppm6A in mammals and m7GpppA in dengue virus, and we did not detect cap m1A/m1Am in humans. CapQuant accurately captured the preference for purine nucleotides at eukaryotic transcription start sites and the correlation between metabolite levels and metabolite caps. The mystery around cap m1A/m1Am analysis remains unresolved.

Published
2019
Full Text
View/download PDF

2. N6-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis

Author

Lu Liu, Hao Yu, Weiling Maggie Cai, Zhi Wei Norman Teo, Xingliang Hou, Zhe Liang, Ying Chen, Lisha Shen, Xiaofeng Gu, and Peter C. Dedon

Subjects
0106 biological sciences, 0301 basic medicine, Adenosine, Meristem, Arabidopsis, RNA-binding protein, 01 natural sciences, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Gene Expression Regulation, Plant, RNA Processing, Post-Transcriptional, Molecular Biology, Genetics, Messenger RNA, biology, Arabidopsis Proteins, Methyltransferase complex, Stem Cells, MRNA modification, fungi, Gene Expression Regulation, Developmental, RNA-Binding Proteins, food and beverages, RNA, Cell Biology, biology.organism_classification, Cell biology, Phenotype, 030104 developmental biology, RNA, Plant, Mutation, Shoot, Carrier Proteins, Plant Shoots, 010606 plant biology & botany, Developmental Biology
Abstract

N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification on mRNA and requires a multicomponent m(6)A methyltransferase complex in mammals. How their plant counterparts determine the global m(6)A modification landscape and its molecular link to plant development remain unknown. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m(6)A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lacking FIP37 exhibit massive overproliferation of shoot meristems and a transcriptome-wide loss of m(6)A RNA modifications. We further demonstrate that FIP37 mediates m(6)A RNA modification on key shoot meristem genes inversely correlated with their mRNA stability, thus confining their transcript levels to prevent shoot meristem overproliferation. Our results suggest an indispensable role of FIP37 in mediating m(6)A mRNA modification, which is required for maintaining the shoot meristem as a renewable source for continuously producing all aerial organs in plants.

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results