1. Screening and identification of major phytochemical compounds in seeds, sprouts and leaves of Tuscan black kale Brassica oleracea (L.) ssp acephala (DC) var. sabellica L
- Author
-
Lucia Giorgetti* a, Gianluca Giorgi b, Edoardo Cherubini b, Pier Giovanni Gervasi a, Clara Maria Della Croce a, Vincenzo Longo a, Lorenza Bellani a, and c
- Subjects
0301 basic medicine ,molecular fingerprintings ,Indoles ,Flavonoid ,Phytochemicals ,antioxidant activity ,Secondary Metabolism ,Plant Science ,Tuscan black kale, seeds, sprouts, phytochemical composition, antioxidant activity, molecular fingerprintings ,Biochemistry ,Antioxidants ,Mass Spectrometry ,Analytical Chemistry ,chemistry.chemical_compound ,Imidoesters ,Oximes ,Food science ,chemistry.chemical_classification ,Glucoraphanin ,biology ,Chemistry ,food and beverages ,04 agricultural and veterinary sciences ,Hydroxycinnamic acid ,040401 food science ,Phytochemical ,Sulfoxides ,Seeds ,Brassica oleracea ,Coumaric Acids ,Glucosinolates ,Brassica ,Glucobrassicin ,03 medical and health sciences ,0404 agricultural biotechnology ,Sinapine ,Botany ,Flavonoids ,030109 nutrition & dietetics ,Organic Chemistry ,Polyphenols ,sprouts ,biology.organism_classification ,phytochemical composition ,Plant Leaves ,Glucose ,Polyphenol ,Seedlings ,Tuscan black kale ,seed - Abstract
We report the spectrophotometric determination of total polyphenols, flavonoids, glucosinolates and antioxidant activity in seeds, seedlings and leaves of Tuscan black kale. The highest content of phytochemicals was observed in 10 days sprouts and antioxidant activity was maximum in 2, 4 days seedlings. Identification and characterisation of phytochemicals were performed by mass spectrometry (MS), high resolution and tandem MS with electrospray ionisation mode. Low-molecular-weight metabolites were evidenced in seeds while metabolites at high m/z range were detected in cotyledons and leaves. MS spectra evidenced different phenolic compounds (flavonoid caffeoyl glucose, hydroxycinnamic acid sinapine) and glucosinolates (glucoerucin, glucobrassicin and glucoraphanin) in function of developmental stage; galactolipids ω3 and ω6 were observed in leaves. Identification of stages with the highest phytochemicals content encourages the consumption of black kale sprouts and young leaves. Our research can support food and pharmaceutical industries for production of health promoting products from black kale.
- Published
- 2017
- Full Text
- View/download PDF