Search

3D Reconstructions is an important application of computer graphics and images processing in biomedicine engineering. Marching Cube algorithm is the most representative structure in the face of 3D reconstructions. We present new aspects of form representation based exclusively on 3D analytical tools and procedures. Our method is applied to a set of 21 unworn upper second molars belonging to eight extant anthropoid genera. Using geometrical analysis of polygonal meshes representatives of the tooth form, we propose a 3D dataset that constitutes a detailed characterization of the enamel and of the enamel–dentine junction morphologies. Therefore, methods of three-dimensional (3D) reconstructions were employed to visualize the surface morphology of developing structures and to help appreciate the complexity of early tooth morphogenesis. The authors introduce in the paper the principle of MC algorithm, and present a simplified algorithm based on optimized grid model. The simplified algorithm selects points as close to original grid as possible, usually using the subset selection method or the optimized selection method. The rudiments can rescue their suppressed development and revitalize, and then their subsequent autonomous development can give rise to oral pathologies. This shows that tooth-forming potential in mammals can be greater than that observed from their functional dentitions. From this perspective, the mouse rudimentary tooth primordia represent a natural model to test possibilities of tooth regeneration.

Our long-term objective is to isolate and characteristics human dental pulp stem cells (hDPSCs) derived from human third molar pulp. We demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Another aim was to verify the expression with dentin sialoprotein (DSPP), dentin sialoprotein (DSP), dentin matrixprotein 1 (DMP-1) and vimentin. For characterisation, proliferation capacity, phenotypic properties, and differentiation characteristics were utilised. Stem cells isolated from hNDP were analysed by flow cytometry and immunocytochemistry. Thus, both EMPs are important during hDPSCs proliferation. During cytodifferentiation, EMPs are essential for the complete differentiation of odontoblasts by up-regulating the expression of DSPP, DSP, DMP-1 and vimentin. Stimulation of these cells by EMPs significantly increases alkaline phosphatase(ALP) activity. ALP activity was expressed as OD 405 nm/mg of protein A increase was observed in ALP activity of hDPSCs during the culturing of EMPs. According to the differentiation and proliferation potential of HDPSC was shown. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.

The aim of this study was to isolate and characteristics human dental pulp stem cells (hDPSCs) derived from human third molar pulp. Another aim was to verify the expression with dentin sialoprotein (DSPP), dentin sialoprotein (DSP), dentin matrixprotein 1 (DMP-1) and vimentin. For characterisation, proliferation capacity, phenotypic properties, and differentiation characteristics were utilised. Stem cells isolated from hNDP were analysed by flow cytometry and immunocytochemistry. Cell line were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. The expression of DSPP, DSP, DMP-1and vimentin, as analyzed by Immunocytochemistry, increased after EMPs incubating for 5d. So exhibit multilineage differentiation properties. Thus, both EMPs are important during hDPSCs proliferation. During cytodifferentiation, EMPs are essential for the complete differentiation of odontoblasts by up-regulating the expression of DSPP, DSP, DMP-1 and vimentin. Stimulation of these cells by EMPs significantly increases alkaline phosphatase(ALP) activity. ALP activity was expressed as OD 405 nm/mg of protein A increase was observed in ALP activity of hDPSCs during the culturing of EMPs. According to the differentiation and proliferation potential of HDPSC was shown. A specific function for EMPs up-regulat in differentiation of hDPSCs was elucidated.

3D Reconstructions has been widely used in the field of medical disease diagnosis and Marching Cube algorithm is the most representative structure in the face of 3D reconstructions. Therefore, methods of three-dimensional (3D) reconstructions were employed to visualize the surface morphology of developing structures and to help appreciate the complexity of early tooth morphogenesis. The authors introduce in the paper the principle of MCalgorithm, and present a simplified algorithm based on optimized grid model. The simplified algorithm selects points as close tooriginal grid as possible, usually using the subset selection method or the optimized selection method. The rudiments can rescue their suppressed development and revitalize, and then their subsequent autonomous development can give rise to oral pathologies. This shows that tooth-forming potential in mammals can be greater than that observed from their functional dentitions. From this perspective, the mouse rudimentary tooth primordia represent a natural model to test possibilities of tooth regeneration.