1. Production and characterization of poly(3-hydroxy butyrate-co-3 hydroxyvalerate) (PHBV) by a novel halotolerant mangrove isolate
- Author
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Kesavan Madhavan Nampoothiri and Dhanya Moorkoth
- Subjects
0301 basic medicine ,Arabinose ,Environmental Engineering ,Sucrose ,Polyesters ,Proton Magnetic Resonance Spectroscopy ,030106 microbiology ,Lignocellulosic biomass ,Bacillus ,Bioengineering ,Xylose ,Polyhydroxyalkanoates ,03 medical and health sciences ,chemistry.chemical_compound ,Acetic acid ,Halogens ,RNA, Ribosomal, 16S ,Spectroscopy, Fourier Transform Infrared ,Monosaccharide ,Biomass ,Food science ,Pentanoic Acids ,Waste Management and Disposal ,Phylogeny ,Waste Products ,chemistry.chemical_classification ,Calorimetry, Differential Scanning ,Renewable Energy, Sustainability and the Environment ,General Medicine ,Carbon ,Saccharum ,030104 developmental biology ,chemistry ,Biochemistry ,Wetlands ,Propionate ,Nanoparticles ,Propionates - Abstract
A halophilic mangrove isolate identified by 16S rRNA sequence as a Bacillus spp. was found to be capable of using a broad range of carbon sources including monosaccharides (glucose and fructose), disaccharides (sucrose), pentoses (xylose and arabinose), various organic acids (acetic acid, propionic acid and octanoic acid) and even the acid pre-treated liquor (APL) of sugarcane trash, a lignocellulosic biomass, for growth and the production of polyhydroxyalkanoates (PHAs) such as poly(3-hydroxybutyrate, P3HB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate, PHBV), and 4-hydroxyhexanoate, 4HHX). The study describes the innate ability of a wild-type culture for PHBV production by both propionate dependent and propionate independent pathways. The biopolymer was extracted and characterized physico-chemically. The PHBV yield from glucose was estimated to be 73% of biomass weight with a high 3-hydroxyvalerate fraction of 48mol%. Thereafter, spherical homogenous PHBV nanoparticles of ∼164nm size were prepared for future applications.
- Published
- 2016